IgG receptors on the mast cell

Abstract

Antisera to the IgG subclasses, 1, 2a, 2b, and 2c, induced histamine release from mast cells obtained from the peritoneal washings of Lister hooded rats. The maximum responses obtained with anti-IgG1 and anti-IgG2a were as great as that for anti-IgE (more than 60% histamine release). Cells from unresponsive Wistar rats which did not secrete appreciable amounts of histamine in response to any of the antisera, produced on active sensitization with ovalbumin a small but significant response on challenge with anti-IgG1 and anti-IgG2b as well as with anti-IgE. Passive sensitization with rat myeloma serum of mast cells from the unresponsive rats produced a large response on challenge with anti-IgE but no release to the anti-IgG group 2 subclasses. IgE myeloma serum (1:1000) neutralized the histamine-releasing activity on anti-IgE serum (87% inhibition) and the antisera to all subclasses of IgG. When the IgE in the myeloma serum was inactivated by heating, the response to the IgG antisera remained completely inhibited except for anti-IgG2a where some reversal was observed. When purified myeloma IgE (30 microgram/ml) was used in place of whole serum, marked inhibition (86%) of the response to anti-IgE was obtained leaving the responses to the IgE subclasses unaffected (except for IgG2a, which was 65% inhibited).

Effects of Inhibitors of Anaphylactic Mediators in Two Models of Bronchial Anaphylaxis in Anaesthetized Guinea‐pigs

“…As there are reports of IgG-mediated release of anaphylactic mediators like histamine and SRS-A from rat peritoneal mast cells (Moodley & Mongar, 1981) and guinea-pig lung in vitro (Andersson & Bergstrand, 1981;Forsberg & Sorenby, 1981), it seems reasonable to assume the release of histamine and SRS-A induced by IgG antibodies in the present investigation to be derived from mast cells. There are indications of separate receptors for IgG and IgE antibodies on mice mast cells (Daeron, ProuvostDanon & Voisin, 1980).…”

A comparison of histamine secretion from peritoneal mast cells of the rat and hamster

“…These results indicate a high degree of cross-reactivity between and within the hamster and rat immunoglobulins but prevent determination of the nature of the antibody involved in the release process. Since both IgG or IgE a f 0 * 0 may sensitize the mast cell for mediator release (for references see Moodley & Mongar, 1981) either or both antibodies may be implicated in the present response. Consistently, rat peritoneal cells also reacted to both anti-hamster serum and to anti-rat IgE.…”

Antigen‐induced Bronchial Anaphylaxis in Actively Sensitized Guinea‐pigs: Effect of Long‐term Treatment With Sodium Cromoglycate and Aminophylline

“…There are reports indicating different receptors for IgG and IgE mast cells (Daeron, Pronovost-Danon & Voisin, 1980;Moodley & Morgar 1981). Andersson (1980a) showed that much higher provocation doses must be used to induce a bronchial response in guinea-pigs sensitized according to procedure (b) compared with those sensitized according to procedure (a).…”