IgG subclass of anti-HSV antibodies following neonatal HSV infections

Hayward, Anthony R.; Herberger, Mark; Corey, Lawrence

doi:10.1007/bf00439394

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…A cellular immunodeficiency was excluded, and this is well illustrated by the appearance of regular IgM and IgG antibodies specific for HSV II during the course of the disease, thus demonstrating immunocompetence. Recently, it has been described that neutralising HSV antibodies need to reach a critical serum level to prevent HSV infection [15,29,30]. There is no doubt that T-cell mediated immune reactions involving cytotoxic T-cells are of major importance [6,17].…”

Section: Discussionmentioning

confidence: 99%

Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis

Benador¹,

Mannhardt

Schranz

et al. 1990

Eur J Pediatr

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We report three cases of neonatal herpes simplex virus (HSV) infection presenting as fulminant hepatitis. None of the patients had clear risk factors for HSV infection and they all died. Antiviral treatment for HSV is currently available but must be administered early in the course of the disease before irreversible liver tissue damage is present. Since the diagnosis may be difficult to establish, we wish to draw the attention of clinicians to the presentation of neonatal HSV infection and suggest that in such cases viral cultures, including culture of liver tissue, should be obtained early and antiviral treatment administered while awaiting the culture results.

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Section: Discussionmentioning

confidence: 99%

Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis

Benador¹,

Mannhardt

Schranz

et al. 1990

Eur J Pediatr

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“…#30-875 N and 30-876N, Walkersville, MD) diluted 1:20 in PBS, and then blocked with 1% gelatin. Sera diluted 1:10-1:1000 were incubated on the wells for 30 min, subsequently washed with Tris-buffered saline with 0.1% Tween, incubated with peroxidase-conjugated antihuman isotype antibodies (Cappel, Organon Teknika, Cat #55226, Durham, NC) and substrate developed as previously described [16]. The data were log converted and the results expressed as a percentage of a pooled human immunoglobulin reference.…”

Section: Elisa Test For Rsv Antibodymentioning

confidence: 99%

Respiratory Syncytial Virus Infects the Bonnet Monkey, Macaca radiata

et al. 1999

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The Bonnet monkey model of respiratory syncytial virus (RSV) infection may be a useful nonhuman primate model for studying RSV disease in humans because Bonnet monkeys can predictably be infected to obtain an orderly sequence of morphologic, cytologic, virologic, serologic, and inflammatory changes related to time of infection. Young feral Bonnet monkeys, Macaca radiata, were infected endotracheally with 10(6) plaque-forming units (pfu) of the Long strain of RSV. RSV was recovered from the animals' lungs at necropsy on days 3, 5, and 7 with the highest viral titer obtained on day 3 (1.1 and 5.2 x 10(3) pfu/g of tissue in the upper and lower lobes, respectively). RSV antigen and F protein mRNA were detected 3-5 days after infection in alveolar macrophages and in the epithelium of bronchi, terminal bronchioles, and alveoli. Histologic analysis of RSV-infected lungs at necropsy revealed progressive bronchiolar mucosal and submucosal inflammation, periarterial mononuclear interstitial inflammation, and focal alveolitis, with a maximal response at 7 days after infection. Cell counts in bronchoalveolar lavage (BAL) increased with time with neutrophils and macrophages predominating on day 3 (6.47 and 5.85 x 10(5)/mm3, respectively) and lymphocytes predominating on day 9 (4.18 x 10(5)/mm3). Serum-neutralizing antibody appeared on day 5 and IgG antibody to RSV was detected on day 9. This sequence of morphologic, cytologic, virologic, serologic, and inflammatory change following RSV infection creates a useful model in the study of experimentally induced RSV disease with a potential for testing future vaccine-induced alterations in RSV disease response.

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“…Blood was obtained from 17 consenting healthy adults, defibrinated, and centrifuged to recover serum. Three subjects had no history of VZV infection; their blood MNC did not proliferate in cultures stimulated with VZV antigen and their sera were negative for VZV antibody in an enzyme-linked immunosorbent assay (15). Fourteen VZV-immune subjects were studied.…”

Section: Methodsmentioning

confidence: 99%

T-cell responses to predicted amphipathic peptides of varicella-zoster virus glycoproteins II and IV

Hayward

1990

J Virol

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Four peptides from the predicted amino acid sequences of varicella-zoster virus (VZV) glycoproteins II and IV selected for potential amphipathicity and a terminal lysine residue were synthesized. The peptides elicited weak proliferative responses by T cells with the CD4+ UCHL1+ CD45Rphenotype from the blood of VZV-immune individuals. The frequency of responder cells in individuals with specific response to peptides was 1:80,000 or fewer blood mononuclear cells, and the number of peptides responded to did not correlate with the proliferative response to VZV antigen. Of 40 peptide-specific T-cell clones obtained by limiting dilution, 10 were restimulated by extracted VZV antigen in the presence of autologous antigen-presenting cells. A total of 50% of these clones lysed HLA class II-positive lymphoblasts which had been preincubated with the appropriate peptide, and 2 of 15 cytotoxic clones lysed lymphoblast targets superinfected with VZV. The data indicated that T cells with specificity for putative VZV peptides may readily be cultured from the subset of blood mononuclear cells which bears the phenotype associated with memory.

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IgG subclass of anti-HSV antibodies following neonatal HSV infections

Cited by 6 publications

References 6 publications

Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis

Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis

Respiratory Syncytial Virus Infects the Bonnet Monkey, Macaca radiata

T-cell responses to predicted amphipathic peptides of varicella-zoster virus glycoproteins II and IV

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