IgG Subclass Response to<i>Helicobacter pylori</i>in Patients with Chronic Active Gastritis and Duodenal Ulcer

Bontkes, Hetty J.; Veenendaal, R.A.; Peña, A. S.; Goedhard, Jelle G.; Duijn, Wim van; Kuiper, Jonas J.W.; Meijer, J. L.; Lamers, C. B. H. W.

doi:10.3109/00365529209165432

Cited by 26 publications

(22 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported by others (23,24), in the present study we also observed that IgG concentration in adults does not permit the discrimination between gastritis and duodenal ulcer. We have no explanation for the observation that the IgG concentration in the sera from females with gastritis was significantly higher than in males with gastritis.…”

Section: Diagnosis Of H Pylori Infection By Cobas Core Elisasupporting

confidence: 73%

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

Rocha¹,

Oliveira²,

Queiroz³

et al. 1998

Braz J Med Biol Res

View full text Add to dashboard Cite

We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative. Antibodies were detected by Cobas Core anti-H. pylori EIA in 62 of 65 H. pylori-positive adults and in none of the negative adults. The sensitivity, specificity and positive and negative predictive values of the test were 95.4, 100, 100 and 91.4%, respectively. The Cobas Core anti-H. pylori EIA presented high sensitivity and specificity when employed for a population in Brazil, permitting the use of the test both to confirm the clinical diagnosis and to perform epidemiologic surveys.

show abstract

Section: Diagnosis Of H Pylori Infection By Cobas Core Elisasupporting

confidence: 73%

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

Rocha¹,

Oliveira²,

Queiroz³

et al. 1998

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Th1 cells that produce gamma interferon are able to promote the production of IgG2 opsonizing and complement-fixing antibodies. An IgG subclass response to H. pylori in patients with chronic active gastritis and duodenal ulcer was reported by Bontkes et al (5). They indicated that the titers of IgG1 antibodies to the bacteria were raised in patients with H. pylori infection and that the titers of IgG2 antibodies in duodenal ulcer patients were higher than those in gastritis patients.…”

Section: Vol 8 2001mentioning

confidence: 85%

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Ishii

Yokota

Sugiyama

et al. 2001

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is related to Helicobacter pylori infection. Specifically, it has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa heat shock protein (hsp60). To investigate humoral immune responses to the H. pylori hsp60 in patients with gastroduodenal diseases and patients with MALT lymphoma, the hsp60 of H. pylori was expressed with a glutathione S-transferase fusion protein and was purified (recombinant hsp60). Sera were obtained from H. pylori-positive patients with gastroduodenal diseases (MALT lymphoma, n ‫؍‬ 13; gastric ulcer, n ‫؍‬ 20; duodenal ulcer, n ‫؍‬ 20; gastritis, n ‫؍‬ 20) and from H. pylori-negative healthy volunteers (n ‫؍‬ 9). Sera from patients with MALT lymphoma were also obtained at two times: before and after eradication therapy. Antibodies to hsp60 and H. pylori were assessed by enzyme-linked immunosorbent assay. The levels of immunoglobulin G (IgG) antibodies to the hsp60 of H. pylori-positive patients with gastroduodenal diseases were significantly elevated compared to those in the controls. The levels of IgG1 antibodies to hsp60 were elevated and correlated with the levels of anti-H. pylori antibodies in patients with MALT lymphoma. Humarol immunity against hsp60 may be important and relevant to gastroduodenal diseases induced by H. pylori infection.Helicobacter pylori is now recognized as a cause of gastritis, peptic ulcer, gastric cancer, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma (9, 19). Several H. pylori pathogenic factors, including CagA and VacA, may contribute to the gastric mucosal damage (6, 7). In recent years, it has also been accepted that host immune reactions play an important role in the pathogenesis of H. pylori infection. Although H. pylori is a noninvasive bacterium and is restricted to gastric epithelial cells, infection with H. pylori induces humoral and cellular immune responses in the gastric mucosa (16,22). Bacterial attachment causes induction of interleukin-8 (IL-8) release, and Cag proteins evoke activation of nuclear factor-B and release of IL-8 that may support leukocyte attachment during inflammation (8,17,21). However, the bacterial antigens associated with inflammation are still not clear.Heat shock proteins (hsp's) are immunodominant antigens in various diseases including H. pylori infection (4, 26). Our previous studies have shown that the 60-kDa hsp (hsp60) is expressed in the follicular dendritic cells of the gastric mucosa in patients with gastric MALT lymphoma (15) and that antibodies to human hsp60 can be detected in MALT lymphoma patients (14). These results indicate that hsp60 may be associated with the host immune reaction in H. pylori infection, specifically, the pathogensis of gastric MALT lymphoma.In the present study, H. pylori hsp60 recombinant protein was expressed, and the levels of immunoglobulin G (IgG), IgG1, IgG2, IgM, and IgA antibodies to hsp60 in sera were measured in patients with gastric ulcer, duodenal ulcer, gastritis, and gastric MALT lympho...

show abstract

“…Instead, their antibody titres may have been too low for detection. Another possibility is that IgGs other than IgG1 and IgG2 may account for the majority of humoral immunity in these patients [10].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, adult duodenal ulcer patients have higher IgG2 titres against whole-cell antigens than gastritis patients [10]. Finally, variations in IgG subclass titres may be due to the age and the fact that different H. pylori isolates can express different subsets of antigens.…”

Section: Introductionmentioning

confidence: 99%

“…However, IgG subclasses are useful for inferring the state of Th1 ⁄ Th2 polarization in individuals [9] because IgG2 is correlated with the Th1 response (inflammatory response and therefore disease progression), while IgG1 levels increase during the Th2 response [10]. While IgG1-4 can be detected in H. pyloriinfected individuals, IgG1 and IgG2 are predominant.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IgG Subclasses Against Helicobacter pylori Isolates: An important Tool for Disease Characterization

et al. 2012

View full text Add to dashboard Cite

Helicobacter pylori infects around 50% of the world’s population and is associated with diverse pathologies. In the most severe cases, the bacterium causes peptic ulcers and gastric cancer. The interplay between H. pylori and the host’s immune response may help to determine the specific outcome of the infection. To study the relationship between antibody subclasses and variation in immune recognition, we determined the immunoglobulin G1 and 2 (IgG1 and IgG2) titres of sera obtained from patients with different H. pylori‐associated pathologies. IgG1 and IgG2 titres were determined by ELISA in 44 sera of patients with different H. pylori‐associated diseases (peptic ulcer, bleeding peptic ulcers, gastric cancer and dyspepsia). Soluble proteins from lysates were obtained from 12 different clinical isolates from similar associated diseases. We found that soluble proteins from lysates of H. pylori strains (SPLHP) recognition patterns in these sera were highly variable. Overall, IgG2 titres were higher than the IgG1 titres in the infected patients. In particular, those with peptic ulcers showed marked elevation in IgG2/IgG1 ratios, while SPLHPs from dyspeptic patients resulted in high IgG1 titres. Our results reveal that correlation of antibody subclass titres with Th1/Th2 markers may aid pathology characterization and show a potential diagnosis that could be formally evaluated in other studies.

show abstract

IgG Subclass Response toHelicobacter pyloriin Patients with Chronic Active Gastritis and Duodenal Ulcer

Cited by 26 publications

References 17 publications

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

IgG Subclasses Against Helicobacter pylori Isolates: An important Tool for Disease Characterization

Contact Info

Product

Resources

About