IgG Subclass Response to Immunization with Haemophilus influenzas Type b Polysaccharide-Outer Membrane Protein Conjugate Vaccine1

Granoff, Dan M.; Weinberg, Geoffrey A.; Shackelford, Penelope G.

doi:10.1203/00006450-198808000-00008

Cited by 41 publications

(14 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, four had detectable IgGl responses (> 0.5 pg/mL) and three had detectable IgG2 responses (> 0.3 pg/mL) ( Table 3). This pattern of IgG subclass responses is similar to that observed previously in children immunized with either Hib PS or Hib PS-protein conjugate vaccines (25). Only one of the immunized children (subject 7) had a subnormal ?Visits to the physician for infection during the 12 mo before the initial blood sample.…”

Section: Resultssupporting

confidence: 71%

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

et al. 1990

Self Cite

View full text Add to dashboard Cite

ABSTRACT. To understand the relevance of subnormal serum concentrations of IgG2, we measured IgG2 in serum of 575 healthy children and identified 11 with concentrations >2 SD less than the mean for age. The levels of IgG2 present were similar to those found in symptomatic children with IgG2 subclass deficiency associated with antibody deficiency. The 11 children ranged in age from 1 to 14 y (mean = 5.7). Detailed clinical information was available on 10 of the 11 children and each was matched for age with two controls. The median number of visitsly to the doctor for infectious illnesses was identical for the two groups (1.0). Nine of the children with subnormal IgG2 were followed for 1 to 5 y (mean = 2.3). All nine children had normal serum concentrations of IgA, IgG1, IgG3, and IgG4 but seven had persistently subnormal or low-normal serum IgG2 concentrations. One of these seven children also had a subnormal serum concentration of IgG, and one had subnormal IgM. Antibody responses to Haemophilus b polysaccharide vaccine were normal in five of six who were immunized. In vitro secretion of Ig by mitogen-stimulated peripheral blood mononuclear cells was measured in six of seven children with persistently subnormal or lownormal IgG2; five showed decreased secretion of IgG2, and two of the five also had subnormal secretion of IgGl and IgG3. An important implication of this study is that the subnormal concentrations of serum IgG2 found in infectionprone children are not a sufficient explanation for their increased susceptibility to infection. The healthy children with low serum concentrations of IgG2 differ from symptomatic children with subnormal IgG2 in that the former have otherwise normal serum Ig concentrations and have normal antibody responses to Hib P S vaccine.

show abstract

Section: Resultssupporting

confidence: 71%

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

et al. 1990

Self Cite

View full text Add to dashboard Cite

show abstract

“…The age distributions of the subjects and the respective antibody responses to vaccination are summarized in Table I. With the exception of 10 ofthe adults, the antibody responses of these subjects were included in previous reports (16)(17)(18)(19).…”

Section: Methodsmentioning

confidence: 99%

A major crossreactive idiotype associated with human antibodies to the Haemophilus influenzae b polysaccharide. Expression in relation to age and immunoglobulin G subclass.

Lucas¹,

Granoff²

1990

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…In contrast, carbohydrate polysaccharide (PS) antigens, T-cell-independent (TI) antigens, preferentially elicit IgG2 in adults (6,25). In infants and children TI responses are predominantly of the IgG1 subclass (9,17). The potential functional differences in the abilities of the IgG subclasses to clear bacteria, however, are not always obvious (24).…”

mentioning

confidence: 99%

Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides

Voer

Klis

Engels

et al. 2008

Clin Vaccine Immunol

View full text Add to dashboard Cite

A fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup A (MenA) and C (MenC) polysaccharides (PS) was developed. The assay comprised three separate duplex assays, one for the detection of the IgG response to MenA and MenC PS, another for the detection of the IgG1 response to MenA and MenC PS, and a third for the detection of the IgG2 response to MenA and MenC PS. Next, the three separate duplex assays were combined and analyzed as a hexaplex assay. No interference between monoplex, duplex, and hexaplex assays was observed, and the assay was found to have low intra-and interassay variation (<9.0% and <27%, respectively). Comparison of the meningococcal subclass MIA to the in-house enzyme-linked inmmunosorbent assays showed a good correlation (R > 0.85) for each of the subclasses. We conclude that the hexaplex meningococcal subclass MIA is an easy and specific assay for the determination of anti-MenA and anti-MenC PS subclass IgG, requiring minimal amounts of serum to study IgG subclass responses to vaccines.

show abstract

IgG Subclass Response to Immunization with Haemophilus influenzas Type b Polysaccharide-Outer Membrane Protein Conjugate Vaccine1

Cited by 41 publications

References 14 publications

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

Clinical and Immunologic Characteristics of Healthy Children with Subnormal Serum Concentrations of IgG2

A major crossreactive idiotype associated with human antibodies to the Haemophilus influenzae b polysaccharide. Expression in relation to age and immunoglobulin G subclass.

Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides

Contact Info

Product

Resources

About