IgG subclasses, barrier function for albumin and production of immunoglobulin G in the central nervous system

Abstract

Cerebrospinal fluids from patients with neurological diseases have been studied. It appeared that the detectability of immunoglobulin G subclasses 1, 2, 3, 4, and the occurrence of oligoclonal bands in the IgG region increased with increasing concentration of IgG in the spinal fluid. No specific pattern emerged in the different disease groups. The reference intervals were determined for: Sp-Albumin(66,OOO): 0.08-4.4 pmol/l; Sp-IgG(160,OOO): 0.06-0.26 pmoV1; S-Albumin : 120413, Sp-AlbuminSp-IgG X S-Albumin within the reference interval, while S-IgG Sp-Albumin was increased. Sp: spinal fluid S-AlbuminSp-IgG X S-Albumin Sp-Albumin S-IgG X Sp-Albumin In these ratios the variability of the concentration of albumin and IgG in serum is taken into account and corrected for.Reference intervals and the values obtained in a variety of neurological diseases for these parameters are presented. and MATERIAL Reference interval samplesCerebrospinal fluid specimens with Sp-Protein (ref. int. 0.15-0.50 g/l), Sp-Erythrocytes and Sp-Leucocytes (ref. int. 0-3 X 106/1) within the reference interval were used to determine the reference intervals for Sp-Albumin(66,000) Sp-Immunoglobulin G S-Albumin Sp-IgG X S-Albumin (160,000), Sp-Albumin and S-IgG Sp-Albumin according to Delpech & Lichtblau (1972) Spinal fluid controlsA pool of 60 reference interval samples of spinal fluid was prepared. The Sp-IgG was 0.15 pmol/l and the Sp-Albumin was 3 pmolll. Part of the pool was concentrated 10 times by dialysis against Carbowax 20 M, and nonconcentrated as well as 10 times concentrated pool was used as control. The pools were stored at -20°C in portions of 1 ml.

Blood-brain barrier integrity in patients with cerebral infarction investigated by computed tomography and serum-CSF-albumin

“…Serum albumin (S-albumin) (66,000) was quantitated by the bromocresol green method at p H 4.2 for higher specificity using Greiner electronics selective analyzer GSA I1 (Dounzas et al 1971, Gustafsson 1976. CSFalbumin (66,000) was quantitated by immunonephelometry on the Technicon 'AIP' Autoanalyzer 11 (Doumas & Biggs 1972, Ritchie et al 1973, Lizana & Hellsing 1974 using rabbit antisera to human serum albumin raised in our laboratory (Al-Kassab et al 1979).…”

Cerebrospinal fluid protein findings in cervical syndromes classified by myelography, and in multiple sclerosis

“…During the past 10 to 15 years in the laboratory diagnosis of neurological diseases, special attention has been paid to the significance of the de-termination of various CSF protein fractions as well as to their relations to each other (Tourtelotte 1970, Delpech & Lichtblau 1973, Link & Tibbling 1977. Investigation of the CSF may also yield information useful in the determination of causes of radicular pain (Delank & Zamani 1975, Al-Kassab et al 1979, Rieder & Fritsci 1980. However, quite contradictory reports have been published in recent years concerning CSF protein patterns in patients with cervical and lumbar discopathies.…”