IgG4 and Passive Sensitization of Basophil Leukocytes

Toorenenbergen, A. W. Van; Aalberse, R. C.

doi:10.1159/000232784

Cited by 50 publications

(13 citation statements)

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“…Human basophils express FcyRII (8), but no FcyRIII, and they failed to release histamine when challenged with anti-FcyRII (C. Lawrence and M. Daeron, unpublished data). Ancient experiments aiming at demonstrating the ability of IgG immune complexes to trigger histamine release in human basophils were contradictory (14,15). Human FcyRII are of two types: FcyRIIA/C and FcyRIIB.…”

Section: Introductionmentioning

confidence: 99%

Regulation of high-affinity IgE receptor-mediated mast cell activation by murine low-affinity IgG receptors.

Daëron¹,

Malbec²,

Latour³

et al. 1995

J. Clin. Invest.

334

225

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Section: Introductionmentioning

confidence: 99%

Regulation of high-affinity IgE receptor-mediated mast cell activation by murine low-affinity IgG receptors.

Daëron¹,

Malbec²,

Latour³

et al. 1995

J. Clin. Invest.

334

225

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“…In later investigations other methods including Prausnitz-Kiistner-test, in vitro sensitization of hu man lung preparations and studies on human baso phils have been used [2, 6, 8 (review), 9,12,13,15,[24][25][26][27]. The results of these studies are far from un equivocal, as both positive and negative evidence for IgG as reaginic antibody have accumulated.…”

Section: Introductionmentioning

confidence: 99%

Role of IgG4 in ï list a mine Release from Human Basophil Leucocytes

Poulsen¹,

Skov²,

Weeke³

1988

Int Arch Allergy Immunol

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Several conflicting reports on the ability of IgG4 to mediate type I allergic reactions have appeared lately. We have developed a model system for testing this possibility, using passive sensitization of basophil leuocytes from normal individuals. At first, the system was optimized with regard to donor cells and anti-IgG4 antibody: among 3 normal individuals with cells showing high, medium and low responses to anti-IgE, only the high responder showed a reproducible, but low response to anti-IgG4. This response was consistent when testing anti-IgG4 from different sources, and could be potentiated by a phorbol ester TPA, although not up to the level of anti-IgE. The cells from the high responding donor and a monoclonal anti-IgG4 were selected for further studies. Serum pools from patients allergic to house dust mite (Dermatophagoides pteronyssinus) were used for passive sensitization. The pools contained allergen-specific IgE with or without concomitant IgG4 of the same specificity. The sensitized basophils reacted to anti-IgE and allergen, but to anti-IgG4 only to a small extent. However, when these serum pools were fractionated by affinity chromatography, only the IgE fractions and not the IgG4 fractions made the cells reactive towards specific allergen. It was still possible to elicit a reaction by triggering IgE with anti-IgE. whereas only a small reaction was seen, when IgG4-sensitized basophils were provoked with antí-IgG4. We conclude that IgG4 does not sensitize normal basophils.

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“…IgG4 has been suggested to have homocytotropic properties (120,121) but these results have not been confirmed by us (95) or others (115,116).…”

Section: Rationales For Utilization Of Igg-allergen Complexesmentioning

confidence: 70%