IgGs-Abzymes from the Sera of Patients with Multiple Sclerosis Recognize and Hydrolyze miRNAs

Ermakov, Evgeny A.; Kabirova, Evelina M.; Buneva, Valentina N.; Nevinsky, Georgy A.

doi:10.3390/ijms22062812

Cited by 8 publications

(6 citation statements)

References 72 publications

(129 reference statements)

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“…It showed earlier that all IgGs and sIgAs effectively hydrolyze four microRNAs: miR-9-5p, miR-219-2-3p, miR-137, and miR-219a-5p, which are found in high concentrations in the blood of patients with autoimmune diseases [ 73 , 74 ]. Elevated concentrations of these four microRNAs are more characteristic of patients with SLE and MS, and IgGs effectively hydrolyzed them from the blood of these patients [ 69 , 70 , 71 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

“…The spatial models of four microRNAs were generated by Predict a Secondary Structure server, which uses a combination of four algorithms for predicting the secondary structure of RNA, similarly to [ 69 , 70 , 71 ]: calculating a partition function, denoting the structure with minimal energy by (accessed on 14 February 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Initially, it was shown that antibodies (abzymes) from the blood of patients with multiple sclerosis [ 69 ], systemic lupus erythematosus [ 70 ], and schizophrenia [ 71 , 72 ] effectively hydrolyze four microRNAs that are characteristic of these diseases: miR-9-5p, miR-219-2-3p, miR-137, and miR-219a-5p. As noted above, human milk contains many different microRNAs, including these four microRNAs.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNAs of Milk in Cells, Plasma, and Lipid Fractions of Human Milk, and Abzymes Catalyzing Their Hydrolysis

Kompaneets¹,

Ermakov²,

Buneva³

et al. 2022

IJMS

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Human milk provides neonates with various components that ensure newborns’ growth, including protection from bacterial and viral infections. In neonates, the biological functions of many breast milk components can be very different compared with their functions in the body fluids of healthy adults. Catalytic antibodies (abzymes) that hydrolyze peptides, proteins, DNAs, RNAs, and oligosaccharides were detected, not only in the blood sera of autoimmune patients, but also in human milk. Non-coding microRNAs (18–25 nucleotides) are intra- and extracellular molecules of different human fluids. MiRNAs possess many different biological functions, including the regulation of several hundred genes. Five of them, miR-148a-3p, miR-200c-3p, miR-378a-3p, miR-146b-5p, and let-7f-5p, were previously found in milk in high concentrations. Here, we determined relative numbers of miRNA copies in 1 mg of analyzed cells, lipid fractions, and plasmas of human milk samples. The relative amount of microRNA decreases in the following order: cells ≈ lipid fraction > plasma. IgGs and sIgAs were isolated from milk plasma, and their activities in the hydrolysis of five microRNAs was compared. In general, sIgAs demonstrated higher miRNA-hydrolyzing activities than IgGs antibodies. The hydrolysis of five microRNAs by sIgAs and IgGs was site-specific. The relative activity of each microRNA hydrolysis was very dependent on the milk preparation. The correlation coefficients between the contents of five RNAs in milk plasma, and the relative activities of sIgAs compared to IgGs in hydrolyses, strongly depended on individual microRNA, and changed from −0.01 to 0.80. Thus, it was shown that milk contains specific antibodies (abzymes) that hydrolyze microRNAs specific for human milk.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MicroRNAs of Milk in Cells, Plasma, and Lipid Fractions of Human Milk, and Abzymes Catalyzing Their Hydrolysis

Kompaneets¹,

Ermakov²,

Buneva³

et al. 2022

IJMS

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“…This reflects the peculiarity of the immunological reactivity of the organism during this disease. Autoantibodies-abzymes-that hydrolyze microRNAs were found in the sera of patients with multiple sclerosis and systemic lupus erythematosus [111,112]. Evidence has been presented that the secretory IgA molecules of breast milk (but not the blood sera of healthy men and non-pregnant women) can effectively hydrolyze ribooligonucleotides, i.e., they are abzymes that effectively hydrolyze microRNAs in their loop and duplex regions.…”

Section: Detection Of Diagnostic Antibodiesmentioning

confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

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“…One of the specific features of AI disease progression was shown to be the appearance of antibodies (abzymes) in the patients’ blood that hydrolyze DNA, RNA, proteins, nucleotides, and oligosaccharides (for review, see [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]). The blood of MS patients was found to contain abzymes that efficiently hydrolyze DNA [ 13 , 14 ], RNA, specific microRNA [ 15 ], the myelin basic protein [ 16 , 17 , 18 ], five histones [ 19 ], and oligosaccharides [ 20 , 21 ]. Interestingly, IgG antibodies from the cerebrospinal fluids of MS patients were 30–60 times more active in hydrolysis of MBP, DNA, and oligosaccharides than from the serum of the same patients [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes

et al. 2022

Self Cite

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The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis.

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IgGs-Abzymes from the Sera of Patients with Multiple Sclerosis Recognize and Hydrolyze miRNAs

Cited by 8 publications

References 72 publications

MicroRNAs of Milk in Cells, Plasma, and Lipid Fractions of Human Milk, and Abzymes Catalyzing Their Hydrolysis

MicroRNAs of Milk in Cells, Plasma, and Lipid Fractions of Human Milk, and Abzymes Catalyzing Their Hydrolysis

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes

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