IgIII (270–280)-fragment-like H2N-DDSDEEN-COOH peptide modulates N-CAM expression via Ca2+-dependent ERK signaling during “in vitro neurogenesis”

Mariggiò, Maria Addolorata; Morabito, Caterina; Guarnieri, Simone; Gentile, Antonietta; Kolkova, Kateryna; Fanò, Giorgio

doi:10.1016/j.peptides.2008.05.009

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…In the S-aggregates, the modelled microgravity conditions did not have any significant effects on the localisation of N-CAM and tyrosine hydroxylase, but they were shown to induce a switch of the GAP43 protein from the neurite-like processes to the cytoplasmic compartment. In addition, the microgravity induced a slight, although not significant, decrease in the expression levels of GAP43 and tyrosine hydroxylase, while it had no effect on the expression of the N-CAM-180 isoform but significantly increased the expression of the N-CAM-140 isoform, which has been shown to have a key role in neuronal survival and signal transduction [ 39 , 40 ]. This suggests the involvement of N-CAM-140 during this modelled microgravity exposure that could promote a significant degree of neuronal remodelling and survival.…”

Section: Discussionmentioning

confidence: 99%

RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes onIn Vitro3D Neuroglial Cell Cultures

Morabito

Steimberg

Mazzoleni

et al. 2015

BioMed Research International

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We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.

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Section: Discussionmentioning

confidence: 99%

RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes onIn Vitro3D Neuroglial Cell Cultures

Morabito

Steimberg

Mazzoleni

et al. 2015

BioMed Research International

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“…That these expressions were altered in opposite directions was somewhat surprising, as NCAM expression is dependent on ERK signaling during neurogenesis, at least in vitro (20). However, these changes in expression were evaluated at 1 month after SE generation; thus, it is not known what shorter term effects may have been involved.…”

Section: Discussionmentioning

confidence: 99%

“…Also not investigated were the possible dose-dependent effects of these pharmacological agents, or if shorter or longer term use may have altered the results. Finally, NCAM expression is dependent on ERK signaling during neurogenesis, at least in vitro (20); studies on NCAM1 and ERK2 co-expression in the same or different cell types also warrants investigation.…”

Section: Discussionmentioning

confidence: 99%

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Effects of pharmacological treatments on hippocampal NCAM1 and ERK2 expression in epileptic rats with cognitive dysfunction

et al. 2016

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The present study aimed to investigate the effects of various pharmacological agents on the hippocampal expression of neural cell adhesion molecule 1 (NCAM1) and extracellular signal-regulated kinase 2 (ERK2) in epileptic rats with cognitive dysfunction. The experiments were conducted using 120 Wistar rats: 20 controls and 100 with pilocarpine-induced status epilepticus (SE). The SE rats were randomly assigned to 5 groups (n=20/group) that received daily treatments for 1 month with one of the following: (i) saline (no effect on epilepsy); (ii) carbamazepine (an anticonvulsant); (iii) oxcarbazepine (an anticonvulsant); (iv) aniracetam (a nootropic); or (v) donepezil (an acetylcholinesterase inhibitor). Spatial learning and memory were assessed using a Morris Water Maze (MWM). Hippocampal tissue was assessed for NCAM1 and ERK2 messenger RNA (mRNA) expression by reverse transcription polymerase chain reaction, and protein expression by immunochemistry. The results revealed that SE rats had significantly poorer MWM performances compared with controls (P<0.01). Performance in SE rats was improved with donepezil treatment (P<0.01), but declined with carbamazepine (P<0.01). Compared with controls, saline-treated SE rats exhibited increased hippocampal NCAM1 mRNA expression (P<0.01). Among SE rats, NCAM1 mRNA expression was highest in those treated with donepezil, followed by aniracetam-, saline-, oxcarbazepine- and carbamazepine-treated rats. Compared to controls, saline-treated SE rats exhibited decreased hippocampal ERK2 mRNA expression (P<0.01). Among SE rats, ERK2 mRNA expression was highest in those treated with donepezil, followed by aniracetam, saline, oxcarbazepine and carbamazepine. NCAM1 and ERK2 protein expression levels were parallel to those of the mRNA. In saline-treated SE rats, hippocampal ERK2 expression was decreased and NCAM1 expression was increased; thus, these two molecules may be involved in the impairment of spatial memory. Carbamazepine augmented this impairment, whereas donepezil was found to ameliorate the dysfunction associated with epilepsy. In conclusion, ERK2 and NCAM1 have significant roles in impairment of spatial memory in SE rats. Carbamazepine may increase this impairment, while donepezil may decrease this impairment.

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Interaction of DDSDEEN peptide with N-CAM protein. Possible mechanism enhancing neuronal differentiation

et al. 2008

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IgIII (270–280)-fragment-like H2N-DDSDEEN-COOH peptide modulates N-CAM expression via Ca2+-dependent ERK signaling during “in vitro neurogenesis”

Cited by 4 publications

References 26 publications

RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes onIn Vitro3D Neuroglial Cell Cultures

RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes onIn Vitro3D Neuroglial Cell Cultures

Effects of pharmacological treatments on hippocampal NCAM1 and ERK2 expression in epileptic rats with cognitive dysfunction

Interaction of DDSDEEN peptide with N-CAM protein. Possible mechanism enhancing neuronal differentiation

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