IgM antibody against measles virus in patients with inflammatory bowel disease: a marker of virus-related disease?

Balzola, F.; Castellino, F.; Colombatto, P.; Manzini, Paola; Astegiano, Marco; Verme, G; Brunetto, M.R.; Pera, A.; Bonino, Ferruccio

doi:10.1097/00042737-199707000-00003

Cited by 19 publications

(9 citation statements)

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“…Others have reported a 78% seroprevalence of measles specific IgM in CD patients, 59% seroprevalence in UC, 10% among patients with hepatitis C, and 0% blood donor controls. 19 An IBD population-based epidemiologic study from Manitoba, Canada, interestingly reported an inversion of the gender ratio, with an excess of male cases among birth cohorts born between 1967 and 1982. 20 Although the implications from this observation are uncertain, a notable differ- ence between females and males born after 1967 in Manitoba is that adolescent females, and not males, were the target of a province-wide rubella vaccination program between 1979 and 1995.…”

Section: Discussionmentioning

confidence: 99%

Population-based case-control study of measles, mumps, and rubella and inflammatory bowel disease

Bernstein

Rawsthorne

Blanchard

2007

Inflammatory Bowel Diseases

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Section: Discussionmentioning

confidence: 99%

Population-based case-control study of measles, mumps, and rubella and inflammatory bowel disease

Bernstein

Rawsthorne

Blanchard

2007

Inflammatory Bowel Diseases

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“…Recently, some investigators suggested that both measles and mumps infections during the same year up to age 10 were associated with a substantially increased risk for both UC and CD (87). However, con icting data have been published concerning the isolation of measles virus, serologic ndings and epidemiological studies (79,(88)(89)(90)(91)(92)(93).…”

Section: Paramyxovirusmentioning

confidence: 99%

The Bacterial Flora in Inflammatory Bowel Disease: Current Insights in Pathogenesis and the Influence of Antibiotics and Probiotics

Linskens

Huijsdens

Savelkoul

et al. 2001

Scandinavian Journal of Gastroenterology

194

122

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Linskens RK, Huijsdens XW, Savelkoul PHM, Vandenbroucke-Grauls CMJE, Meuwissen SGM. The bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics. Scand J Gastroenterol 2001;36 Suppl 234:29-40.The pathogenesis of in ammatory bowel disease (IBD) remains unknown, although in recent years more data have become available. The contribution of genetic and environmental factors is evident, and the luminal bacterial ora plays a major role in the initiation and perpetuation of chronic IBD. Animal models of IBD have shown that colitis does not occur in a germ-free environment. In human IBD, in ammation is present in parts of the gut containing the highest bacterial concentrations. Moreover, the terminal ileum, caecum and rectum are areas of relative stasis, providing prolonged mucosal contact with luminal contents.Enhanced mucosal permeability may play a pivotal role in maintaining a chronic in ammatory state, due to a genetic predisposition or as a result of direct contact with bacteria or their products. A defective epithelial barrier may cause a loss of tolerance to the normal enteric ora. Furthermore, an increased mucosal absorption of viable bacteria and bacterial products is found in IBD. Serum and secreted antibodies are increased and mucosal T-lymphocytes that recognize luminal bacteria are present. However, there is evidence that the immune system reacts over aggressively towards the normal luminal ora rather than the ora being altered in IBD. Several approaches have been used in attempts to discover a speci c microbial agent in the cause of IBD. These include demonstration of the presence of organisms or speci c antigens in affected tissues, culture of microbes from the affected tissues, demonstration of serological responses to several agents, and localization and detection of individual pathogen-speci c nucleic acid sequences in affected tissue by in situ hybridization and polymerase chain reaction. So far, no speci c micro-organism has been directly associated with the pathogenesis of IBD. Analysis of the luminal enteric ora, however, has revealed differences in the composition of this ora compared to healthy controls. In Crohn disease, concentrations of Bacteroides, Eubacteria and Peptostreptococcus are increased, whereas Bi dobacteria numbers are signi cantly reduced. Furthermore, in ulcerative colitis, concentrations of facultative anaerobic bacteria are increased. The arrival of new molecular techniques qualifying and quantifying the complex intestinal ora has induced a revival of interest in this micro ora.Therapeutic approaches geared towards changing the environment at the mucosal border have been attempted by the use of elemental diets, total parenteral nutrition, surgical diversion of the faecal stream and antibiotics. Over the past few years, the use of probiotics in IBD and other intestinal disorders has gained attention. Strengthened by promising experimental data and commercial interests, research in this eld is rapidly expandin...

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“…At present, immunological investigation of the potential link between measles and CD has been limited to the assessment of anti-measles antibody levels in patients with CD compared to controls [14,49-51]. Equipped with sophisticated bioinformatic approaches, computer searches are now able to identify linearized molecular mimics.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic and immunological analysis reveals lack of support for measles virus related mimicry in Crohn’s disease

Polymeros

Tsiamoulos

Κουτσουμπασ

et al. 2014

BMC Med

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BackgroundA link between measles virus and Crohn’s disease (CD) has been postulated. We assessed through bioinformatic and immunological approaches whether measles is implicated in CD induction, through molecular mimicry.MethodsThe BLAST2p program was used to identify amino acid sequence similarities between five measles virus and 56 intestinal proteins. Antibody responses to measles/human mimics were tested by an in-house ELISA using serum samples from 50 patients with CD, 50 with ulcerative colitis (UC), and 38 matched healthy controls (HCs).ResultsWe identified 15 sets of significant (>70%) local amino acid homologies from two measles antigens, hemagglutinin-neuraminidase and fusion-glycoprotein, and ten human intestinal proteins. Reactivity to at least one measles 15-meric mimicking peptide was present in 27 out of 50 (54%) of patients with CD, 24 out of 50 (48%) with UC (CD versus UC, p = 0.68), and 13 out of 38 (34.2%) HCs (CD versus HC, p = 0.08). Double reactivity to at least one measles/human pair was present in four out of 50 (8%) patients with CD, three out of 50 (6%) with UC (p = 0.99), and in three out of 38 (7.9%) HCs (p >0.05 for all). Titration experiments yielded different extinction curves for anti-measles and anti-human intestinal double-reactive antibodies. Epitope prediction algorithms and three-dimensional modeling provided bioinformatic confirmation for the observed antigenicity of the main measles virus epitopic regions.ConclusionsMeasles sequences mimicking intestinal proteins are frequent targets of antibody responses in patients with CD, but this reactivity lacks disease specificity and does not initiate cross-reactive responses to intestinal mimics. We conclude that there is no involvement of measles/human molecular mimicry in the etiopathogenesis of CD.Electronic supplementary materialThe online version of this article (doi:10.1186/s12916-014-0139-9) contains supplementary material, which is available to authorized users.

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IgM antibody against measles virus in patients with inflammatory bowel disease: a marker of virus-related disease?

Cited by 19 publications

References 0 publications

Population-based case-control study of measles, mumps, and rubella and inflammatory bowel disease

Population-based case-control study of measles, mumps, and rubella and inflammatory bowel disease

The Bacterial Flora in Inflammatory Bowel Disease: Current Insights in Pathogenesis and the Influence of Antibiotics and Probiotics

Bioinformatic and immunological analysis reveals lack of support for measles virus related mimicry in Crohn’s disease

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