IgM antibody capture haemadherence test (MACHAT) for the detection of measles specific IgM

Siqueira, Marilda Mendonça; Ferro, Z.A.; Cohen, B. J.; Brown, David; Nascimento, Jussara Pereira do

doi:10.1016/0166-0934(94)90173-2

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“…Because of these difficulties with IgG detection, various researchers working with U.S. Copyright Clearance Center Code Statement: 0931-184X/99/4602-0115 $14.00/0 CDV and other morbilliviruses, mainly measles, tried several diagnostic approaches to detect IgM in the place of IgG, thus demonstrating an ongoing or recent viral multiplication (THOM- SON et al, 1975;BRINGUIER et al, 1978;ERDMAN et al, 1991ERDMAN et al, , 1993BLIXENKRONE-MÖ LLER et al, 1991;SIQUEIRA et al, 1994;ARISTA et al, 1995). In our experiments we used a recombinant N protein of the virulent A75/17 CDV strain as antigen.…”

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confidence: 99%

Detection of IgM Antibodies Against a Recombinant Nucleocapsid Protein of Canine Distemper Virus in Dog Sera using a Dot‐Blot Assay

Barben

Stettler

Jaggy

et al. 1999

Journal of Veterinary Medicine Series A

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A dot‐blot assay for the detection of IgM antibodies (ABs) against canine distemper virus (CDV) in canine serum is described. The diagnostic potential of this technique was evaluated by analysing sera from three test groups: (i) specific pathogen‐free (SPF) beagle dogs experimentally infected with virulent CDV; (ii) SPF dogs immunized with a combined vaccine containing CDV, and (iii) SPF dogs immunized with a CDV‐free vaccine. As antigen for the dot‐blot assay we used the recombinant nucleocapsid protein (N protein) of the virulent A75/17 CDV strain. All 12 dogs of group 1, infected with virulent CDV, showed detectable CDV‐specific IgM levels in their serum. All dogs of group 2 were also positive for anti‐CDV IgM after the first immunization with the CDV‐containing vaccine. The four dogs immunized with a CDV‐free vaccine (group iii) remained negative throughout the course of the experiment. From these results, we conclude that the IgM detection test, which requires only a single serum sample, is a useful method for diagnosing current or recent CDV infection in CDV‐infected or CDV‐immunized dogs under experimental conditions.

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