IgM Complex Receptors on Subpopulations of Murine Lymphocytes

In the culture medium of some human lymphoblastoid cell lines material is released with the following properties: (a) hemagglutination reaction of IgG-sensitized erythrocytes; (b) enhancement of precipitation of DNA-anti-DNA complexes; (c) inhibition of binding of C1q to immune complexes; (d) inhibition of immune complex binding to lymphocytes; (e) inhibition of antibody-dependent lymphocytotoxicity. The material is not identical with C1q or rheumatoid factor, it is heat resistant (30 min at 56 degrees C); the molecular weight is about 100 000 daltons and it is capable of inhibiting antibody production in vitro. It is suggested that this material consists of Fc receptors spontaneously shed from lymphocyte membranes.

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Spontaneous release of Fc receptor‐like material from human lymphoblastoid cell lines

Molenaar

Galen

Hannema

et al. 1977

Eur J Immunol

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Fc receptors on rabbit lymphocytes Existence of receptors for IgG antibody complexed with antigen; conditions for its detection

Bast¹,

Manten-Slingerland²,

Roholl³

et al. 1980

Eur J Immunol

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The presence of Fc receptors (FcR) on rabbit peripheral blood leukocytes is demonstrated using rosette formation with an ox erythrocyte-antibody (EoxA) complex. The receptor is specific for the Fc fragment of IgG (neither IgM nor F (ab')2 anti-Eox mediates rosette formation) that is antigen-bound (aggregated rabbit IgG inhibits the rosette formation only transiently). The receptor is species-specific: guinea pig IgG/Eox, goat IgG/Eox and sheep IgG/Eox complexes do not show rosette formation, and goat IgG aggregates do not inhibit rosette formation. The origin of the target erythrocytes is of importance. Sheep erythrocytes are not useful, and within Eox large differences between donors were found. Rosette formation was only inhibited by pretreatment of the rosette-forming cells with homologous immune complexes, whereas the size of the antigen greatly influenced the degree of inhibition. The rabbit FcR is pronase-resistant, unlike the human and murine RcR. The interaction of IgG and the FcR is not inhibited by isolated C gamma 3 domains. Further evidence for the requirement of the whole Fc region was obtained in experiments where inhibition of the rosette formation was observed using antisera directed to the C gamma 3 and the C gamma 2 domain, respectively. Anti-Fab antiserum did not inhibit rosette formation. Results are discussed in relation to the mechanism of allotypic suppression.

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Fc receptors on rabbit lymphocytes. Identification and organ distribution of rosette‐forming cells; cocapping with surface immunoglobulin

Bast¹,

Manten-Slingerland²,

Roholl

et al. 1980

Eur J Immunol

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Fc receptor (FcR)-bearing cells were demonstrated using ox erythrocytes coated with homologous IgG-type antibodies (EA gamma) in rabbit peripheral blood leukocytes (PBL) and in various lymphoid organs. Discrimination of the rosette-forming cells (RFC) is carried out after prior ingestion of tetramethylrhodamine isothiocyanate-labeled latex particles and in transmission electron microscopic studies. Most of the nonlymphoid cells (5-10%) in PBL and spleen cell suspensions expose FcR. These nonlymphoid cells are almost absent in other lymphoid organs, except in bone marrow. The average percentage of cells rosetting with IgG-sensitized erythrocytes (EA gamma RFC) in lymphoid cell preparations of the various tissues was as follows: PBL 25%, bone marrow 65%, appendix 37%, spleen 40%, Peyer's patches 44%, thymus 2% and peripheral lymph node 27%. The nature of FcR-bearing PBL was further studied using F (ab')2 anti-IgM, anti-IgA or anti-T cell conjugates. About half of the population of B cells, bearing IgM or IgA express FcR. Moreover, about 80% of the RFC are found within the B cell population. Only a few T cells were found rosetting with EA gamma suggesting that most of the non-B lymphoid RFC are "null" cells. In different lymphoid organs, the percentages of EA gamma RFC and B cells are comparable but not identical A greater part of the EA gamma RFC also expresses the receptor for the third component of complement. After capping of membrane IgM determinants, FcR is located in the same cap on the majority (60%) of the FcR-positive IgM-capped cells.

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IgM Complex Receptors on Subpopulations of Murine Lymphocytes

Cited by 51 publications

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Spontaneous release of Fc receptor‐like material from human lymphoblastoid cell lines

Spontaneous release of Fc receptor‐like material from human lymphoblastoid cell lines

Fc receptors on rabbit lymphocytes Existence of receptors for IgG antibody complexed with antigen; conditions for its detection

Fc receptors on rabbit lymphocytes. Identification and organ distribution of rosette‐forming cells; cocapping with surface immunoglobulin

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