Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

Gupta, Arvind; Dharne, Mahesh; Rangrez, Ashraf Yusuf; Verma, Pankaj; Ghate, Hemant V.; Rohde, Manfred; Patole, Milind S.; Shouche, Yogesh S.

doi:10.1099/ijs.0.018622-0

Cited by 63 publications

(59 citation statements)

References 48 publications

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“…To identify the oxalate positive isolate OX_R, morphological characterization, 16S rRNA gene sequencing and assessment of production of cell associated reddish pigment were performed. Genomic DNA from OX_R strain was isolated, 16S rRNA gene sequencing and phylogenetic analysis of derived sequence was carried out as method described earlier (Gupta et al, 2011). Based on the analysis, the strain OX_R was identified to be Serratia marcescens.…”

Section: Isolation and Identification Of Oxalotrophic Serratia Sppmentioning

confidence: 99%

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

Suryavanshi¹,

Waghmode²,

Bharti³

et al. 2016

J App Pharm Sci

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Prodigiosin a multifaceted secondary metabolite produced by Serratia spp. having great potential as a pharmaceutical. In the present study we demonstrate that oxalate supplementation in peptone glycerol production media increased organoleptic characters and yield of prodigiosin pigment extracted from oxalotrophic Serratia marcescens OX_R isolated from Indian bat guano sample. The pigment was demonstrated in-vitro as an antibacterial agent against common opportunistic skin surface pathogen Staphylococcus aureus NCIM 5021 strain as killing activity by agar well diffusion method. The docking analysis and pharmacophore modelling indicated that the probable mechanism of action of the prodigiosin was against Staphylococcus aureus DNA gyrase protein. The pigment was also found to efficiently dye both cotton and latex polymer. In summary, we describe here an oxalotrophic Serratia marcescens which may serve as a potent and economical resource of prodigiosin which owing to its dyeing and anti-bacterial activities finds future avenues to be developed as dressing material for nosocomial subjects or burn victim patients.

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Section: Isolation and Identification Of Oxalotrophic Serratia Sppmentioning

confidence: 99%

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

Suryavanshi¹,

Waghmode²,

Bharti³

et al. 2016

J App Pharm Sci

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“…The genus Ignatzschineria (formerly known by the illegitimate name Schineria) belongs to the class Gammaproteobacteria and comprises three recognized species: I. indica, I. larvae, and I. ureiclastica (8,9). All three species have been isolated from the obligate parasitic fly Wohlfahrtia magnifica (flesh fly; Diptera: Sacrophagidae), but we believe this is the first report of I. indica being associated with the blowfly.…”

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confidence: 99%

“…The members of this genus are aerobic, Gram-negative, non-spore-forming, nonhemolytic, nonmotile, rod-shaped bacteria. Colonies are small (0.05 to 0.2 mm), nonpigmented, entire, convex, and translucent, grow on blood agar, MacConkey agar, and Salmonella-Shigella (SS) agar, and are catalase and oxidase positive (9). Prior to our cases, the primary etiological agents reported in cases of myiasis were Wohlfahrtiimonas chitiniclastica and I. larvae, each associated with the parasitic fly Wohlfahrtia magnifica.…”

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confidence: 99%

First Case Reports of Ignatzschineria (Schineria) indica Associated with Myiasis

et al. 2014

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We report three cases of infection due to the Gram-negative rod Ignatzschineria (Schineria) indica involving bacteremia and the urinary tract. Two cases were clearly associated with maggot infestation, and the third could conceivably have had unrecognized maggot infestation of the urinary tract. We believe these cases to be the first I. indica infections reported in association with maggot infestation and myiasis. CASE REPORTSC ase 1 is a 64-year-old homeless male who presented to the emergency department at the University of Louisville Hospital with the chief complaint of a painful left foot. His pertinent medical history included a motor vehicle accident 2 months prior to this admission, at which time he sustained lacerations to the dorsal aspect of the first three digits of his left foot. Because of his social situation, he had been unable to treat his wounds or change the dressings since the accident. He complained of extreme pain in the foot, which was exacerbated with any movement or pressure. He reported no other symptoms or past medical history. On physical examination, his left foot was edematous and erythematous surrounding the bandages. Following removal of the dressings, the wound revealed malodorous lacerations located on the dorsum of the foot and along the border of digits 1, 2, and 3 which expressed serous drainage. Maggots were observed in the wound and between the digits. All pedal pulses were palpable. Vital signs and the remainder of the physical examination were unremarkable.Laboratory studies revealed a normal white blood cell count (8,800/l with 64.2% granulocytes), an elevated erythrocyte sedimentation rate (ESR [57 mm/h]) and elevated C-reactive protein (CRP) level (1.06 mg/dl). X ray of the left foot demonstrated mild dorsal soft tissue swelling with no acute fracture or dislocation. However, magnetic resonance imaging (MRI) of the foot showed a fracture of the third middle phalanx with adjacent soft tissue defect. The clinical impression was osteomyelitis, although not seen on imaging, and the patient was started on empirical ampicillin-sulbactam (3 g intravenous [i.v.] every 6 h [q6h]) and vancomycin (1.25 g i.v., q12h). His wounds were redressed wet to dry with Dakin's solution, and the necrotic tissue was debrided with removal of the maggots. Despite conservative treatment, the third digit was considered unsalvageable, and the patient was taken to surgery for amputation of the digit. Histopathology noted skin ulceration and prominent acute and chronic inflammation extending to the soft tissue margin. On the second day postadmission, two aerobic blood cultures were positive for nonhemolytic Gram-negative short plump rods. The isolate produced a "yellowish" pigment on blood agar. The oxidase and indole tests were, respectively, positive and negative. The organism was identified as Alcaligenes faecalis (97% probability) (RapidID NF Plus; Remel, Lenexa, KS). Attempts to perform susceptibility testing were unsuccessful due to the organism's not growing in the Microscan Gram-negative pane...

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“…Ignatzschineria is found associated with predominantly the samples from environment and insects such as flesh fly and human infections are rarely reported. Three species were recently reported in association with human infection: I. larvae, I. indica and I. ureiclastica [1]. In 2007, Roudiere et al reported the isolation of Schineria (former name of Ignatzschineria) larvae from the foot of a 39-year-old homeless man with medical history of polyneuropathy related to alcohol abuse [2].…”

Section: Discussionmentioning

confidence: 99%

“…The genus Ignatzschineria is characterized by aerobic, gramnegative, non-spore forming, non-motile and regular rodshaped bacteria, and Ignatzschineria indica was described in 2011 using 16S rRNA gene sequencing by Gupta et al [1]. I. indica has been isolated from the gut content of adult flesh flies, Sarcophaga (Dipteria: Sarcophagidae).…”

Section: Introductionmentioning

confidence: 99%

Ignatzschineria indica Isolated From a Human Breast Abscess: A Rare Case

et al. 2016

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Ignatzschineria indica is a gram-negative aerobic bacillus, usually isolated from the gut content of adult flesh flies or larvae of Wohlfahrtia magnifica. Ignatzschineria spp. associated with human infection is hypothesized to be related to maggot therapy. We are reporting the first case of Ignatzschineria indica isolated from a breast abscess, along with Proteus penneri and Providencia stuartii in a 76-year-old female. The patient presented with a left breast mass, weight loss, and normal vital signs; physical examination of the left breast showed a fungating mass with foul smelling draining necrotic tissue and a left axillary abscess. A biopsy was performed that revealed an invasive mammary carcinoma (mucinous type) with the tumor ulcerating the overlying skin. Additionally, a culture of the abscess yielded growth of gram-negative rods. The organism was identified as I. indica by 16S rRNA gene sequencing. Although the pathogenic capacity of the organism cannot be determined at this point, we are establishing the need for further study of patients with cultures positive for I. indica in order to understand the pathologic implications, and therapy to control this organism in human host.

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Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

Cited by 63 publications

References 48 publications

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

First Case Reports of Ignatzschineria (Schineria) indica Associated with Myiasis

Ignatzschineria indica Isolated From a Human Breast Abscess: A Rare Case

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