II: Effect of Cooling Rate and Duration of Freezing Point Plateau on Boar Semen Frozen in Mini- and Maxi-Straws and Plastic Bags

Bwanga, C. O.; Einarsson, S.; Rodríguez‐Martínez, H.

doi:10.1186/bf03546945

Cited by 31 publications

(3 citation statements)

References 7 publications

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“…The higher post thaw sperm motility obtained with controlled rate of freezing methods in this study was thought to be less detrimental effect of it on sperm function, compared to conventional freezing method. This is consistence with the previous studies (Bwanga et al 1991, Petyim et al 2007, Kaeoket et al 2008. The disruption of plasma membrane of spermatozoa resulting from rapid and uncontrolled rate of cooling in conventional freezing method led to substantial reduction of sperm with intact plasma membrane.…”

Section: Effect On Sperm Motility Viability Plasma Membrane Integrity...supporting

confidence: 89%

A comparative study of effects of different freezing methods on sperm quality, DNA integrity and membrane protein of cryopreserved boar semen*

Biswas¹,

Kadirvel

Deka³

et al. 2018

Indian J of Anim Sci

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The present study determined the effects of conventional, and controlled freezing method adopting three freezing rates, viz. 20°C, 40°C and 60°C/min on quality of sperm (motility, viability and plasma membrane integrity), DNA integrity and plasma membrane protein profile of cryopreserved boar semen. Sixty sperm-rich fractions of ejaculates from six boars were utilized for freezing of semen with different freezing methods in lactose-egg yolk glycerol extender. Semen samples were evaluated for sperm motility, viability (Propidium Iodide assay), functional integrity of plasma membrane (HOST), DNA integrity (Acridine Orange stain) and plasma membrane protein profile (SDSPAGE) after equilibration and after freezing. The results revealed that the post thaw sperm motility, sperm viability, and plasma membrane integrity (HOST-reacted) were significantly higher in all the three controlled freezing methods (20°C, 40°C and 60°C/min) as compared to that in conventional method. In addition, the number of sperm plasma membrane protein loss was less in controlled freezing methods as compared to that in conventional freezing. However, the post thaw sperm DNA integrity did not influence by difference in freezing methods. No significant difference on the post thaw sperm characteristics was recorded among the three controlled freezing rates. All the sperm parameters assessed declined significantly after freezing as compared to that after equilibration irrespective of freezing method employed. In conclusion, controlled freezing methods conferred better post thaw sperm quality as compared to conventional method, and thus the freezing rates of either 20, 40 or 60°C/min could provide better freezability of boar semen.

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Section: Effect On Sperm Motility Viability Plasma Membrane Integrity...supporting

confidence: 89%

A comparative study of effects of different freezing methods on sperm quality, DNA integrity and membrane protein of cryopreserved boar semen*

Biswas¹,

Kadirvel

Deka³

et al. 2018

Indian J of Anim Sci

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“…Considering that brown‐marbled grouper is characterized by large body and broodstocks with good maturity have high fecundity, small straws may have limitations for large‐scale artificial insemination in practice; it would be labour and time consuming for sperm packaging and thawing since numerous straws have to be handled at the same time. Although sperm freezing with large volume has many advantages in practice, its potential for use may be limited which can cause longer freezing point plateau and less uniform cooling rate in the container, resulting in compromised post‐thaw sperm quality (Bwanga et al, 1991). As both types of container and frozen volume can influence the heat exchange efficiency, it is necessary to adjust the freezing method when scaling up the storage volume (Nomura et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Yang

Fan

Chen

et al. 2022

Aquaculture Research

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Brown‐marbled grouper (Epinephelus fuscoguttatus) is a large‐bodied coral reef fish with high ecological and economic value in South‐East Asia. Previous studies have developed cryopreservation protocols in 0.25‐ or 0.5‐ml straws, but this small volume may limit the utilization of cryopreserved sperm for large‐scale artificial fertilization, considering the high fecundity of brown‐marbled grouper. To aid its commercial application, the main objective of the present study was to further elaborate cryopreservation protocol in this species, by evaluating the cryoprotective efficacy of different sugar extenders (glucose, sucrose or trehalose) and the feasibility of a 5‐ml cryotube for cryopreservation. After frozen in a simple media containing 10% DMSO and 0.3‐M sugar extender using a 0.5‐ml straw, there was a deterioration in sperm motility, viability, mitochondrial membrane potential (MMP) and ATP content compared with fresh semen, but fertilization success of cryopreserved samples was not compromised at the ratio of 10,000 spermatozoa per egg. Besides, the type of sugar extender had no obvious effect on post‐thaw sperm quality and fertility, thus it is suggested that glucose, sucrose or trehalose are fully interchangeable for the cryopreservation of brown‐marbled grouper semen. By employing a faster freezing rate (freezing height: 2 cm vs. 3 cm) and longer thawing time (40°C, 90 s vs. 40°C, 7 s), samples cryopreserved in a 5‐ml cryotube exhibited similar post‐thaw motility parameters and fertility with those in 0.5‐ml straw. Overall, our results demonstrate the effectiveness of new sugar extenders and the applicability of a 5‐ml cryotube for sperm cryopreservation in brown‐marbled grouper, which would allow for better flexibility for large‐scale artificial fertilization in hatchery practice.

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“…Sperm survival in such conditions can be modified by the rate at which they are cooled (Fiser and Fairfull, 1990;Bwanga et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Effect of Genetic Group, Semen Diluents and Freezing Regimens on Sperm Freezability and Goats Reproductivity

Mohammed¹,

Khalil

Al-Saef

2012

Assiut Veterinary Medical Journal

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Two experiments were designed to study the effect of bucks breeds, semen extenders and freezing regimens on post-thaw semen motility and viability index (experiment 1). In experiment 2, semen diluents effects on reproductive performance was conducted. Semen was collected from Aradi (A), Damascuss (D) and cross (½A½D) bucks breeds. Good quality semen was divided into 4 portions, each diluted with one diluent (Milk, Na. Citrate, Tris and Na. Bicarbonate). The diluted semen was packaged into 0.5 ml straws then cooled to 5C. After equilibration, half of the packaged straws were suspended 15 cm above liquid nitrogen (LN) for 15 min. (Freezing regimen1; slow). Other half of straws was suspended at height 15 and 5 cm of LN for 10 and 5 min, respectively (Freezing regimen2; rapid) before plunged into LN. Frozen semen was thawed for post-thaw motility and viability. In the second experiment, semen with good quality was extended with three types of extenders (Milk, Na. Citrate and Tris). Diluted semen were cooled to 5C and used for AI. Results revealed that, pre-freeze semen motility was significant higher in Tris, Na. Citrate and Na. Bicarbonate than milk diluent. Post-thaw semen motility and viability were highly significant for milk and Na. Citrate than Tris and Na. Bicarbonate diluents. Post-thaw semen motility was significantly higher in Aradi and Damascus than crossbred. Post-thaw semen motility and viability revealed, significant higher means for Freezing regimen 2 than regimen 1. Milk and Tris diluents were significantly higher than Na. Citrate for fertility, fecundity and prolificacy. Aradi does was higher than Damascus does in fertility, fecundity and prolificacy. Also, Aradi does was significantly higher than ½A½D does in fertility, while the reverse trend was observed in fecundity and prolificacy. It was concluded that, regarding to post-thaw semen motility, semen viability, fertility%, fecundity% and prolificacy%, milk diluent is preferable than Tris and Na. citrate. Fertility rate of Aradi goat breed is higher than Damascus and crossbred (½A½D).

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II: Effect of Cooling Rate and Duration of Freezing Point Plateau on Boar Semen Frozen in Mini- and Maxi-Straws and Plastic Bags

Cited by 31 publications

References 7 publications

A comparative study of effects of different freezing methods on sperm quality, DNA integrity and membrane protein of cryopreserved boar semen*

A comparative study of effects of different freezing methods on sperm quality, DNA integrity and membrane protein of cryopreserved boar semen*

Evaluation of different sugar extenders and large volume cryotube for sperm cryopreservation of brown‐marbled grouper ( Epinephelus fuscoguttatus )

Effect of Genetic Group, Semen Diluents and Freezing Regimens on Sperm Freezability and Goats Reproductivity

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