III-5 Cytoplasmic Streaming

Kamiya, Nobuo

doi:10.1247/csf.9.supplement_s81

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…Both, active self-assembly and molecular robotics, are clearly nanoscience, in being intellectually stimulating and not immediately applicable. But beyond the potential technological importance, engineering such systems provides us with the concepts and experiences needed to understand the diverse biological roles [100][101][102][103][104][105] of biomolecular motors in a systems context. 106…”

Section: Overcoming the Trade-off Between Stability And Complexitymentioning

confidence: 99%

Self-assembly driven by molecular motors

Hess

2006

Soft Matter

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Section: Overcoming the Trade-off Between Stability And Complexitymentioning

confidence: 99%

Self-assembly driven by molecular motors

Hess

2006

Soft Matter

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“…In the present excitotoxicity paradigm, we measured ADC decreases of the same order of magnitude as those that occur after brain ischemia (Albers, 1998 31 P-MRS demonstrated the absence of energy depletion during the ADC decrease, suggesting that a loss of energy-dependent cytoplasmic streaming is not a major determinant of diminished intracellular bulk water motion and decreased intracellular water ADC. It should be noted in this respect that Ca 2+ overload associated with NMDA-receptor overstimulation and opening of voltage-sensitive Ca 2+ channels can interfere with cytoplasmic circulation, independent of energy failure (Kamiya, 1984;Stearns, 1982). In a recent study using the Xenopus oocyte, however, it was shown that diffusion of ions, water, and several other small molecules was consistent with Brownian motion (Sehy et al, 2002).…”

Section: Adc Decrease After Namentioning

confidence: 99%

In Vivo Excitotoxicity Induced by Ouabain, a Na+/K+-ATPase Inhibitor

Veldhuis¹,

Stelt²,

Delmas³

et al. 2003

Journal of Cerebral Blood Flow & Metabolism

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“…In addition to the above mechanisms, overall displacements in the cytosol could be reduced as a result of diminished cytoplasmic streaming. It has been demonstrated that cytoplasmic streaming is halted at low ATP levels, low pH values, and elevated intracellular Ca 2 + concentrations (Kamiya, 1984), all of which can be direct consequences of cere bral ischemia or excitotoxicity. Finally, it should be stressed that the involvement of the above-mentioned factors in the intracellular diffusion changes is only speculative, as these structural and functional cytosolic alterations were not actually measured in this study.…”

Section: 6mentioning

confidence: 99%

Changes in the Diffusion of Water and Intracellular Metabolites after Excitotoxic Injury and Global Ischemia in Neonatal Rat Brain

Dijkhuizen

Graaf

Tulleken³

et al. 1999

J Cereb Blood Flow Metab

107

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The reduction of the apparent diffusion coefficient (ADC) of brain tissue water in acute cerebral ischemia, as measured by diffusion-weighted magnetic resonance imaging, is generally associated with the development of cytotoxic edema. However, the underlying mechanism is still unknown. Our aim was to elucidate diffusion changes in the intracellular environment in cytotoxic edematous tissue. The ADC of intracellular metabolites was measured by use of diffusion-weighted 1H-magnetic resonance spectroscopy after (1) unilateral N-methyl-D-aspartate (NMDA) injection and (2) cardiac arrest-induced global ischemia in neonatal rat brain. The distinct water ADC drop early after global ischemia was accompanied by a significant reduction of the ADC of all measured metabolites (P < 0.01, n = 8). In the first hours after excitotoxic injury, the ADC of water and the metabolites taurine and N-acetylaspartate dropped significantly (P < 0.05, n = 8). At 24 and 72 hours after NMDA injection brain metabolite levels were diminished and metabolite ADC approached contralateral values. Administration of the NMDA-antagonist MK-801 1.5 hours after NMDA injection completely normalized the water ADC but not the metabolite ADC after 1 to 2 hours (n = 8). No damage was detected 72 hours later and, water and metabolite ADC had normal values (n = 8). The contribution of brain temperature changes (calculated from the chemical shift between the water and N-acetylaspartate signals) and tissue deoxygenation to ischemia-induced intracellular ADC changes was minor. These data lend support to previous suggestions that the ischemia-induced brain water ADC drop may partly be caused by reduced diffusional displacement of intracellular water, possibly involving early alterations in intracellular tortuosity, cytoplasmic streaming, or intracellular molecular interactions.

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III-5 Cytoplasmic Streaming

Cited by 5 publications

References 19 publications

Self-assembly driven by molecular motors

Self-assembly driven by molecular motors

In Vivo Excitotoxicity Induced by Ouabain, a Na+/K+-ATPase Inhibitor

Changes in the Diffusion of Water and Intracellular Metabolites after Excitotoxic Injury and Global Ischemia in Neonatal Rat Brain

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