IL-12 enhances protective immunity in mice engendered by immunization with recombinant 14 kDa Schistosoma mansoni fatty acid-binding protein through an IFN-γ and TNF-α dependent pathway

Fonseca, Cristina Toscano; Brito, Cristiana Fa; Alves, José Bento; Oliveira, Sérgio C.

doi:10.1016/j.vaccine.2003.07.010

Cited by 79 publications

(88 citation statements)

References 33 publications

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“…This is consistent with previous report showing that IFN-␥ is involved in differential isotype secretion, since it enhances IgG2a while suppressing IgG1 production (27). In addition, coadministration of antigens and IL-12 to mice induced a Th1-type immune response through elevation of IFN-␥ levels and consequently increasing Ag-specific IgG2a titers (17,54). The high IFN-␥ levels found in Ribiϩ6AgϩIFN-␥-immunized mice are therefore responsible for the antibody isotype switching toward the Th1-type IgG2a isotype.…”

Section: Discussionsupporting

confidence: 93%

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

2005

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In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-␥) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant systems were used separately or in combination for immunization with the recombinant antigens. Our results demonstrate that immunization of mice with Ribi emulsified antigens in the presence of IFN-␥ (Ribi؉6Ag؉IFN-␥) resulted after challenge with a virulent M. tuberculosis strain in a significant reduction in the CFU counts that was comparable to that achieved with the BCG vaccine (ϳ0.9-log protection). Antigen-specific immunoglobulin G (IgG) titers in the Ribi؉6Ag؉IFN-␥-immunized mice were lower than in mice immunized with Ribi؉6Ag and were oriented toward a Th1-type response, as confirmed by elevated IgG2a levels. In addition, splenocyte proliferation, IFN-␥ secretion, and NO production were significantly higher in splenocytes derived from Ribi؉6Ag؉IFN-␥-immunized mice, whereas IL-10 secretion was decreased. These findings confirm the induction of a strong cellular immunity in the vaccinated mice that correlates well with their enhanced resistance to M. tuberculosis. The adjuvant effect of IFN-␥ was dose dependent. A dose of 5 g of IFN-␥ per mouse per immunization gave optimal protection, whereas lower or higher amounts (0.5 or 50 g/ mouse) of IFN-␥ failed to enhance protection.

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Section: Discussionsupporting

confidence: 93%

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

2005

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“…Levels of IL-4 in all groups from both trials were less than 10 pg/ml (the minimum limit of detection using this kit is 10 pg/ml). This is consistent with earlier studies with TPI and the 23-kDa integral membrane protein candidate vaccine antigens driving Th1-type but not Th2-type immune responses (9,10). Levels of IL-2 and IFN-␥ for each of the two experiments are presented in Tables 3 and 4, respectively.…”

Section: Vol 16 2009 S Japonicum Cocktail Vaccines Via Different Rsupporting

confidence: 90%

“…Several groups have suggested that cell-mediated immunity involving IFN-␥-activated cells may be one mechanism of protective immunity in the mouse schistosome challenge model (4,9,21). In this regard, in vitro studies have demonstrated that schistosomula, the presumed target stage for both SjC23 and SjCTPI vaccines, were killed by macrophages or endothelial cells activated via combinations of cytokines, including IFN-␥ and IL-12 (33).…”

Section: Discussionmentioning

confidence: 99%

DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica

Dai

Zhu

Harn

et al. 2009

Clin Vaccine Immunol

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“…In addition, using DNA encoding S. mansoni 28 kDa glutathione S-transferase to immunize rats leads to a long-lasting predominance of IgG2a and IgG2b (Dupre et al 1997), as does the p80 subunit of S. mansoni calpain (Siddiqui et al 2003). DNA vaccination and co-delivery of antigens with IL-12 result in amplified IgG2a synthesis and down-regulation of synthesis of IgG1 (Fonseca et al 2004, Siddiqui et al 2003. Interestingly, our study demonstrated that vaccination with rSVLBP induced a predominantly a Th1-type of immune response in mice.…”

Section: Discussionmentioning

confidence: 99%

Recombinant tegumental protein Shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum

Gan

Shen

Wang

et al. 2006

Mem. Inst. Oswaldo Cruz

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A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The affinity purified rSVLBP was used to vaccinate mice. The worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.5% and 47.6% less than that from control mice, respectively (p<0.05). There was also a marked increase in the antibody response in vaccinated mice: the titer of IgG1 and IgG2a, IgG2b in the vaccinated group was significantly higher than that in the controls (>1:6,400 in total IgG). In a comparison of the reactivity of sera from healthy individuals and patients with rSVLBP, recognition patterns against this parasite tegumental antigen varied among different groups of the individuals. Notably, the average titres of anti-rSVLBP antibody in sera from faecal egg-negative individuals was significantly higher than that in sera from the faecal egg-positives, which may be reflect SVLBP-specific protection. These results suggested that the parasite tegumental protein SVLBP was a promising candidate for further investigation as a vaccine antigen for use against Asian schistosomiasis

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IL-12 enhances protective immunity in mice engendered by immunization with recombinant 14 kDa Schistosoma mansoni fatty acid-binding protein through an IFN-γ and TNF-α dependent pathway

Cited by 79 publications

References 33 publications

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica

Recombinant tegumental protein Shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum

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