IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

Derycke, Lara; Zhang, Nan; Holtappels, Gabriële; Dutré, Tineke; Bachert, Claus

doi:10.1016/j.jcf.2011.11.007

Cited by 69 publications

(60 citation statements)

References 30 publications

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“…33 Th17 cells have been shown to increase the amount of neutrophils as well as eosinophils to areas of inflammation and even account for the inflammatory lesions in ulcerative colitis. 38,39 This increase in Th17 cells we found may be 1 of the factors driving polyp formation, and further studies need to be undertaken to characterize the intracellular cytokine profiles of these Th17 cells to get a better understanding of their function in CRS.…”

Section: Discussionmentioning

confidence: 80%

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

Miljkovic

Psaltis

Wormald

et al. 2016

Int Forum Allergy Rhinol

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Section: Discussionmentioning

confidence: 80%

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

Miljkovic

Psaltis

Wormald

et al. 2016

Int Forum Allergy Rhinol

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“…γδ T cells, like innate cells, are also involved in early immune responses, producing proinflammatory cytokines (INF-γ, IL-17, TNF-α), and activate adaptive immune cells [46]. The expression of IL-17 has been reported to be increased in the nasal mucosa of CRSwNP patients and is closely related to the infiltration of eosinophils and neutrophils [47,48,49]. Recently, Xia et al [50] reported that IL-17A plays a crucial role in stimulating the production of MUC5AC and goblet cell hyperplasia through the act1-mediated signalling pathway in CRSwNP patients in China.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Lee

Chang²,

Huang³

et al. 2017

ORL

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Background/Aims: To examine whether γδ T cell is expressed in the nasal mucosa of chronic rhinosinusitis (CRS) patients and its potential association with recurrence of nasal polyps. Methods: Thirty-six patients with CRS with nasal polyps (CRSwNP) and 25 patients with CRS without nasal polyps (CRSsNP) were recruited. Twenty-six patients with other nasal diseases served as controls. The CRSwNP group was divided into the eosinophilic CRSwNP and noneosinophilic CRSwNP groups. The expression of γδ T cells was detected by immunohistochemistry. The expression of each subtype of γδ T cells was detected by using qRT-PCR. All patients underwent nasal endoscopy, and postoperative follow-up lasted over 12 months. CRS patients were evaluated by preoperative VAS scores of symptoms and nasal endoscopy Lund-Kennedy scores. Results: The expression of γδ T cells in the CRSwNP groups was stronger than in the CRSsNP and the control group (p < 0.05, p < 0.05). The expression of Vγ1⁺γδ T cells in the eosinophilic CRSwNP group was higher than that in the CRSsNP group and the control group (p < 0.05, p < 0.05). The expression of γδ T cells was associated with high rate of recurrence, tissue eosinophil infiltration, worse symptom score of nasal obstruction, and higher Lund-Kennedy score (all p < 0.05). Conclusions: Increased expression of γδ T cells in CRSwNP may be associated with recurrence of nasal polyps.

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“…As the characteristic cytokine of Th17 cells, IL-17A has been revealed to play a significant role in regulating inflammation, modulating airway structural cells and stimulating innate immunity to mediate neutrophil recruitment in asthma and other airway diseases [7]. Investigations have revealed that IL-17A can orchestrate local inflammation by inducing the release of proinflammatory cytokines, such as TNF-α, IL-1β, G-CSF and IL-6, as well as the chemokines CXCL2 and IL-8, produced by human bronchial fibroblasts, epithelial cells and airway smooth muscle cells [8]–[10]. Furthermore, IL-17A can act in synergy with IL-6 to induce the expression of the mucus proteins MUCAC and MUC5B [11].…”

Section: Introductionmentioning

confidence: 99%

Interleukin-17A Promotes MUC5AC Expression and Goblet Cell Hyperplasia in Nasal Polyps via the Act1-Mediated Pathway

Xia

Bai

et al. 2014

PLoS ONE

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BackgroundRecent studies demonstrated that nasal polyps (NP) patients in China and other Asian regions possessed distinct Th17-dominant inflammation and enhanced tissue remodeling. However, the mechanism underlying these observations is not fully understood. This study sought to evaluate the association of interleukin (IL)-17A with MUC5AC expression and goblet cell hyperplasia in Chinese NP patients and to characterize the signaling pathway underlying IL-17A-induced MUC5AC expression in vitro.MethodWe enrolled 25 NP patients and 22 normal controls and examined the expression of IL-17A, MUC5AC and act1 in polyp tissues by immunohistochemical (IHC) staining, quantitative polymerase chain reaction (qPCR) and western blot. Moreover, by using an in vitro culture system of polyp epithelial cells (PECs), IL-17A-induced gene expression was screened in cultured PECs by DNA microarray. The expression of IL-17RA, IL-17RC, act1 and MUC5AC and the activation of the MAPK pathway (ERK, p38 and JNK), were further examined in cultured PECs and NCI-H292 cells by qPCR and western blotting, respectively.ResultsWe found that increased IL-17A production was significantly correlated with MUC5AC and act1 expression and goblet cell hyperplasia in polyp tissues (p<0.05). IL-17A significantly stimulated the expression of IL-17RA, IL-17RC, act1 and MUC5AC, and the activation of the MAPK pathway in cultured PECs and NCI-H292 cells (p<0.05). In addition, IL-17RA, IL-17RC and act1 siRNA significantly blocked IL-17A-induced MUC5AC production in vitro (p<0.05).ConclusionOur results suggest that IL-17A plays a crucial role in stimulating the production of MUC5AC and goblet cell hyperplasia through the act1-mediated signaling pathway and may suggest a promising strategy for the management of Th17-dominant NP patients.

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IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

Abstract: The present study shows that IL-17A has an impact on neutrophil survival in adult nasal polyp disease, but not in nasal polyps from CF patients.

Cited by 69 publications

References 30 publications

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Interleukin-17A Promotes MUC5AC Expression and Goblet Cell Hyperplasia in Nasal Polyps via the Act1-Mediated Pathway

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IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

Abstract: The present study shows that IL-17A has an impact on neutrophil survival in adult nasal polyp disease, but not in nasal polyps from CF patients.

Cited by 69 publications

References 30 publications

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

T regulatory and Th17 cells in chronic rhinosinusitis with polyps

A Retrospective Analysis of &#x03B3;δ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Interleukin-17A Promotes MUC5AC Expression and Goblet Cell Hyperplasia in Nasal Polyps via the Act1-Mediated Pathway

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A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps