IL-17A Polymorphism (rs2275913) and Levels Are Associated With Preeclampsia Pathogenesis in Chinese Patients  

Abstract

BackgroundPreeclampsia (PE) is a pregnant related syndrome affecting both child and mother. Although, the role of various inflammatory molecules in PE has been demonstrated, importance of proinflammatory molecules such as IL-17A, IL-23 are poorly understood. In the present investigation, we tested a possible association of common genetic variants in IL-17A and IL-23 gene with PE. MethodsClinically diagnosed 115 PE patients who reported to the International Peace Maternity and Child Health Hospital were enrolled in the present study. Age, sex-matched 102 pregnant women, and 147 healthy Chinese women were also included. ELISA was used to quantify serum levels of IL-17A and IL-23 in all enrolled subjects. Common genetic polymorphisms in IL-17A (rs 2275913, rs1974226, and rs1974226), IL-23A (rs11171806), and IL-12B (rs3212227) were genotyped by PCR-RFLP or TaqMan probe-based method. ResultsElevated levels of serum IL-17A was observed in PE patients compared to pregnant (P<0.0001) and healthy women (P<0.0001). However, IL-23 levels were comparable among different clinical categories. Furthermore, heterozygous (GA) and minor allele (A) for IL-17A (rs2275913) and IL-23A (rs11171806) were more prevalent in PE patients in comparison to pregnant ladies or healthy women suggesting an essential role in predisposition to PE development. Interestingly, IL-17A (rs 2275913) mutants were linked with elevated IL-17A levels in comparison to wildtype (GG). ConclusionsIL-17A (rs2275913) variants are associated with higher serum levels of cytokine, and predisposed PE development.