IL-1R1 blockade attenuates liver injury through inhibiting the recruitment of myeloid-derived suppressor cells in sepsis

Luo, Minjie; Wang, Hao; Liu, Ke; Liu, Meidong; Tan, Seang‐Lin; Zhu, Yaxi; Zhang, Huali

doi:10.1016/j.bbrc.2022.06.038

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…CD247 is poorly expressed in septic mice [ 47 ] and is a promising target for the prognosis of sepsis in LPS-induced septic mice [ 48 ]. IL1R1 is upregulated in microglial cells of rats with sepsis-associated encephalopathy [ 49 ]; inhibiting IL1R1 can attenuate liver injury in septic mice [ 50 ]. CD28 expression is abnormally downregulated in sepsis, and activation of CD28 helps to improve the survival rate of septic mice [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of qualitative characteristics of immunosuppression in sepsis based on immune-related genes and immune infiltration features

Zeng,

Jian,

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Identification of qualitative characteristics of immunosuppression in sepsis based on immune-related genes and immune infiltration features

Zeng,

Jian,

et al. 2024

Heliyon

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“…The expression of IL-1R1 protein is known to regulate inflammation by facilitating the uptake of IL-1β [ 63 ]. IL1R1 has been implicated in various diseases, including liver injury [ 64 ], kidney injury [ 65 ], ischemic injury [ 36 ] and neurodegeneration [ 66 ]. Here, it was first verified that IL1R1 mediates microglial activation in TN.…”

Section: Discussionmentioning

confidence: 99%

SHED-derived exosomes attenuate trigeminal neuralgia after CCI of the infraorbital nerve in mice via the miR-24-3p/IL-1R1/p-p38 MAPK pathway

Guo,

Fang,

Zhang

et al. 2023

J Nanobiotechnol

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Background Microglial activation in the spinal trigeminal nucleus (STN) plays a crucial role in the development of trigeminal neuralgia (TN). The involvement of adenosine monophosphate-activated protein kinase (AMPK) and N-methyl-D-aspartate receptor 1 (NMDAR1, NR1) in TN has been established. Initial evidence suggests that stem cells from human exfoliated deciduous teeth (SHED) have a potential therapeutic effect in attenuating TN. In this study, we propose that SHED-derived exosomes (SHED-Exos) may alleviate TN by inhibiting microglial activation. This study sought to assess the curative effect of SHED-Exos administrated through the tail vein on a unilateral infraorbital nerve chronic constriction injury (CCI-ION) model in mice to reveal the role of SHED-Exos in TN and further clarify the potential mechanism. Results Animals subjected to CCI-ION were administered SHED-Exos extracted by differential ultracentrifugation. SHED-Exos significantly alleviated TN in CCI mice (increasing the mechanical threshold and reducing p-NR1) and suppressed microglial activation (indicated by the levels of TNF-α, IL-1β and IBA-1, as well as p-AMPK) in vivo and in vitro. Notably, SHED-Exos worked in a concentration dependent manner. Mechanistically, miR-24-3p-upregulated SHED-Exos exerted a more significant effect, while miR-24-3p-inhibited SHED-Exos had a weakened effect. Bioinformatics analysis and luciferase reporter assays were utilized for target gene prediction and verification between miR-24-3p and IL1R1. Moreover, miR-24-3p targeted the IL1R1/p-p38 MAPK pathway in microglia was increased in CCI mice, and participated in microglial activation in the STN. Conclusions miR-24-3p-encapsulated SHED-Exos attenuated TN by suppressing microglial activation in the STN of CCI mice. Mechanistically, miR-24-3p blocked p-p38 MAPK signaling by targeting IL1R1. Theoretically, targeted delivery of miR-24-3p may offer a potential strategy for TN. Graphical Abstract

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Analysis of the Pathogenesis of Gram-Negative Bacterial Sepsis in Rats Under Nano-Body

Li,

Wang,

Liu

et al. 2023

j biomed nanotechnol

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This work aimed to evaluate the effectiveness of several anti-TLR4 nanobody administration techniques in a gram-negative bacterial sepsis (GNBS) rat model. The targeting proteins for TI-Nb2 and TC-Nb6 anti-TLR4 nanobodies were TLR4203-348 and TLR4349-582, respectively. The survival times (STs) of 44 Sprague-Dawley (SD) rats were tracked in the TI-Nb2, TC-Nb6, TI-Nb2+TC-Nb6, and D0 groups (saline control). Besides, the ELISA was utilized to measure the levels of TNF-, IL-1, IL-8, and IL-10 in different groups. An automatic biochemical analyzer was employed to determine the levels of AST, ALT, AMS, CRE, and Urea. Furthermore, the rat liver and kidney tissue samples were stained with hematoxylin-eosin (HE). Cleaved-caspase-3 (CC3) protein expression (PE) in rat tissues was discovered using immunohistochemistry, and the positive unit (PU) value was computed. The TI-Nb2+TC-Nb6 group exhibited a longder ST, lower TNF-α, IL-1β, IL-8, ALT, AST, AMS, CRE, and Urea levels, and a smaller CC3 protein PU value in nucleus and cytoplasm than the TI-Nb2, TC-Nb6, and D0 groups (all P <0.05). The above findings indicated that the combined usage of TI-Nb2 and TC-Nb6 can successfully reduce the expression levels of CC3 protein, biochemical markers, and inflammatory factors. This could protect the liver, kidneys, and other organs and prolong the ST of sepsis rats.

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IL-1R1 blockade attenuates liver injury through inhibiting the recruitment of myeloid-derived suppressor cells in sepsis

Cited by 4 publications

References 43 publications

Identification of qualitative characteristics of immunosuppression in sepsis based on immune-related genes and immune infiltration features

Identification of qualitative characteristics of immunosuppression in sepsis based on immune-related genes and immune infiltration features

SHED-derived exosomes attenuate trigeminal neuralgia after CCI of the infraorbital nerve in mice via the miR-24-3p/IL-1R1/p-p38 MAPK pathway

Analysis of the Pathogenesis of Gram-Negative Bacterial Sepsis in Rats Under Nano-Body

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