IL-2, IL-6 and IL-8 levels remain unaltered in the course of immunosuppressive therapy after renal transplantation

Rysz, Jacek; Kocur, E; Błaszczak, Robert; Bartnicki, Piotr; Stolarek, Robert; Piechota, Mariusz

doi:10.2478/s11536-008-0011-4

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…To explore the effect of non-chemotaxis-inducing cytokines on neutrophil chemotaxis, neutrophils were preincubated with either 0.6 nM IL-2 or 0.4 nM IL-6 and then exposed to an fMLP gradient (Figure A,B). Challenging neutrophils with 0.6 nM IL-2 or 0.4 nM IL-6 has been shown to be effective in activating neutrophils, but also, few nM level elevation of these interleukins are frequently found in individuals with an abnormal immune response. ,− In this section, comparison is performed between neutrophils that were not activated (denoted as “control”) and those that were preactivated by IL-2 or IL-6 (denoted as “IL-2-activated” or “IL-6-activated”). The results indicate that neutrophils move faster when they are preincubated with interleukins (0.29 ± 0.02 for IL-2 ( p = 0.06) and 0.34 ± 0.02 for IL-6 ( p < 0.0001) compared to 0.25 ± 0.01 for control, Figure c), even though MI and CI were the largest in control neutrophils.…”

Section: Resultsmentioning

confidence: 99%

On-Chip Evaluation of Neutrophil Activation and Neutrophil–Endothelial Cell Interaction during Neutrophil Chemotaxis

Kim

Haynes

2013

Anal. Chem.

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Neutrophils are always surrounded by/interacting with other components of the immune system; however, the current mechanistic understanding of neutrophil function is largely based on how neutrophils respond to a single chemical signal in a simplified environment. Such approaches are unable to recapitulate the in vivo microenvironment; thus, cell behavior may not fully represent the physiological behavior. Herein, we exploit a microfluidic model of the complex in vivo milieu to investigate how cell-cell interactions influence human neutrophil migration and surface marker expression. Neutrophil migration against a bacterially-derived chemoattractant (formyl-met-leu-phe, fMLP), with and without pre-activation by interleukins (interleukin-2 or interleukin-6), was evaluated in the presence and absence of endothelial support cells. Pre-activation by interleukins or interaction with endothelial cells resulted in altered migration rates compared to naïve neutrophils, and migration trajectories deviated from the expected movement toward the fMLP signal. Interestingly, interaction with both interleukins and endothelial cells simultaneously resulted in a slight compensation in the deviation – on endothelial cells, 34.4% of untreated neutrophils moved away from the fMLP signal while only 15.2% or 22.2% (IL-2-activated or IL-6-activated) of pre-activated cells moved away from fMLP. Neutrophils interacting with interleukins and/or endothelial cells were still capable of prioritizing the fMLP signal over a competing chemoattractant, leukotriene B4 (LTB4). Fluorescence imaging of individual human neutrophils revealed that neutrophils treated with endothelial cell-conditioned media showed up-regulation of the surface adhesion molecules CD11b and CD66b upon stimulation. On the other hand, CD11b and CD66b down-regulation was observed in untreated neutrophils. These results leverage single cell analysis to reveal that the interaction between neutrophils and endothelial cells is involved in surface marker regulation, and thus, chemotaxis of neutrophils. This study brings new knowledge about neutrophil chemotaxis in the context of cell-to-cell communications, yielding both fundamental and therapeutically relevant insight.

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Section: Resultsmentioning

confidence: 99%

On-Chip Evaluation of Neutrophil Activation and Neutrophil–Endothelial Cell Interaction during Neutrophil Chemotaxis

Kim

Haynes

2013

Anal. Chem.

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“…In the case of three vessel CAD, it was 1.53, 2.33 and 3.55, respectively. However, no studies are available as a direct reference to these data and to best to our knowledge, this study is the first attempt to assess the effect of CRF on the extent of CAD in coronarography patients [16][17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

“…These groups could not have been subject to statistical analysis due to their inadequate size. The effect of DM-2 on the incidence and extent of coronary stenosis may be reevaluated in future studies specifically designed to test this hypothesis [16][17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Coronary artery atherosclerosis in patients with the initial and the early stage of chronic renal failure

2009

Self Cite

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AbstractThe recent clinical data indicate that the initial and the early stages of chronic renal failure (CRF) may lead to increased incidence of cardiovascular complications and increased extent of coronary artery disease (CAD). This retrospective study was aimed to determine the effects of coexisting diabetes mellitus type 2 (DM-2) and the extent of atherosclerosis in coronary vessels in patients with mildly reduced kidney function (glomerular filtration rate GFR = 89–60 ml/min) and moderately reduced kidney function (GFR = 59–30 ml/min). The study patients included 53 subjects with creatinine concentration above 120 μmol/l as a cut-off level for the initial stage and compensated CRF. The distributions of coronary artery stenosis were also analysed with respect to DM-2 coexistence and levels of haemoglobin glikolised (HbA1c). The odds ratio of pathological changes in coronary arteries in patients with GFR = 44–30 ml/min, with respect to the number of affected vessels — only one, more than one or more than two — were 7.22, 4.90 and 3.55, respectively. In CRF patients with GFR = 60–89 ml/min the odds ratio of one, more than one and more than two vessels with stenosis and CAD was 1.93, 1.70 and 1.53, respectively. DM-2 was not related to the risk of significant coronary artery stenosis and did not enhance the pre-existing changes in the study setting. Our results demonstrate that the initial and the early stages of CRF were significant risk factors for coronary stenoses and for enhancing the pre-existing changes.

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The Roles of Immune Molecules in the Acute Post-Transplant Period

Özkızılcık Koçyiğit,

Pehlivan,

Kılıçaslan Ayna

et al. 2024

Journal of Basic and Clinical Health Sciences

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Purpose: Renal transplantation is a therapeutic choice that enhances the quality of life for patients suffering from end-stage renal failure. The objective of this study was to ascertain the alterations in the levels of immune molecules following transplantation and to examine the correlation between these changes and the medical records of the patients. Material and Methods: The gene expression of an immune molecule panel (FOXP3, TNF-α, IFN-γ, IL-18, IL-6, IL-17a, IL-12a, IL-2, IL-10, and TGF-β) in peripheral blood specimens of 30 kidney transplant patients was determined by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) method with SYBR Green Dye. The serum proteins were quantified using Enzyme-Linked ImmunoSorbent Assay (ELISA). Results: TGF-β exhibited the most significant alteration in gene expression levels compared to the levels before transplanting (p

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IL-2, IL-6 and IL-8 levels remain unaltered in the course of immunosuppressive therapy after renal transplantation

Cited by 3 publications

References 14 publications

On-Chip Evaluation of Neutrophil Activation and Neutrophil–Endothelial Cell Interaction during Neutrophil Chemotaxis

On-Chip Evaluation of Neutrophil Activation and Neutrophil–Endothelial Cell Interaction during Neutrophil Chemotaxis

Coronary artery atherosclerosis in patients with the initial and the early stage of chronic renal failure

The Roles of Immune Molecules in the Acute Post-Transplant Period

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