IL-27 Modulates Chemokine Production in TNF-α -Stimulated Human Oral Epithelial Cells

Abstract

Background/Aims: Interleukin-27 (IL-27) is a cytokine which belongs to the IL-12 family. However, the role of IL-27 in the pathogenesis of periodontal disease is uncertain. The aim of this study was to examine the effect of IL-27 on chemokine production in TNF-α-stimulated human oral epithelial cells (TR146). Methods: We measured chemokine production in TR146 by ELISA. We used western blot analysis to detect the phosphorylation levels of signal transduction molecules, including STAT1 and STAT3 in TR146. We used inhibitors to examine the role of STAT1 and STAT3 activation. Results: IL-27 increased CXCR3 ligands production in TNF-α-stimulated TR146. Meanwhile, IL-27 suppressed IL-8 and CCL20 production induced by TNF-α. STAT1 phosphorylation level in IL-27 and TNF-α-stimulated TR146 was enhanced in comparison to TNF-α-stimulated TR146. STAT3 phosphorylation level in IL-27-treated TR146 did not change by TNF-α. Both STAT1 inhibitor and STAT3 inhibitor decreased CXCR3 ligands production. STAT1 inhibitor overrode the inhibitory effect of IL-27 on IL-8 and CCL20 production in TNF-α-stimulated TR146. Meanwhile, STAT3 inhibitor did not modulate IL-8 and CCL20 production. Conclusion: IL-27 might control leukocyte migration in periodontal lesion by modulating chemokine production from epithelial cells.

Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway

“…destruction [2][3][4]. The dynamic balance between osteoblasts and osteoclasts is the critical link in the process of periapical bone remodeling [5].…”

The anti-inflammatory effect of milk and dairy products on periodontal cells: an in vitro approach

“…Gingival fibroblast and oral epithelial cells are suitable to use for in vitro models to study the possible antiinflammatory role of milk and its fermented products [17,18]. With this bioassay, these cells were exposed to aqueous fractions of cow's milk and human milk, as well as fermented milk products with and without IL1 and TNFα.…”

Sulfur‑containing amino acids in aged garlic extract inhibit inflammation in human gingival epithelial cells by suppressing intercellular adhesion molecule‑1 expression and IL‑6 secretion

“…On the other hand, to the best of our knowledge, there are only a few studies available to date demonstrating the TNF-α-induced expression of ICAM-1 in human gingival epithelial cells, particularly Ca9-22 cells (22,25), and the functional mechanisms have not yet been fully clarified. However, it was previously demonstrated that TNF-α also enhanced the expression or secretion of IL-6 and IL-8 proteins in another human gingival epithelial cell line (OBA9) (36) and the oral epithelial cell line, TR146 (37). These findings indicate that Ca9-22 cells are also available to pharmacologically investigate the anti-inflammatory function of AGE, although Ca9-22 cells have been often used as an oral cancer model.…”