IL‐3‐Dependent Early Erythropoiesis Is Stimulated by Autocrine Transforming Growth Factor Beta

Böhmer, Ralph M.

doi:10.1634/stemcells.22-2-216

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…TGF-β, as well as tumor necrosis factor alpha and IFN gamma, is a powerful inhibitor of erythropoiesis both in vivo and in vitro. The suppressive effect of TGF-β on E-lineage cell proliferation is accompanied by an acceleration of terminal maturation (30)(31)(32)(33)(34).…”

Section: Suppressive Effect Of Tgf-β On Proliferation Of Eps/e-meps Imentioning

confidence: 99%

“…Importantly, hEPs never generated MegK cells even under serum-free culture conditions, which allow optimal growth and differentiation of MegK progenitors (48). CD105 is part of the TGF-β receptor complex (29); several studies have revealed an inhibitory effect of TGF-β on E-lineage cell proliferation, causing accelerated terminal maturation by blocking the cell cycle of immature cells (30)(31)(32)(33)(34). However, these studies targeted CD34…”

Section: Cd105mentioning

confidence: 99%

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Prospective isolation of human erythroid lineage-committed progenitors

Mori

Chen

Pluvinage

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Determining the developmental pathway leading to erythrocytes and being able to isolate their progenitors are crucial to understanding and treating disorders of red cell imbalance such as anemia, myelodysplastic syndrome, and polycythemia vera. Here we show that the human erythrocyte progenitor (hEP) can be prospectively isolated from adult bone marrow. We found three subfractions that possessed different expression patterns of CD105 and CD71 within the previously defined human megakaryocyte/ erythrocyte progenitor (hMEP; Lineage

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Section: Suppressive Effect Of Tgf-β On Proliferation Of Eps/e-meps Imentioning

confidence: 99%

Section: Cd105mentioning

confidence: 99%

Prospective isolation of human erythroid lineage-committed progenitors

Mori

Chen

Pluvinage

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…On average, only 77% of the erythroblasts generated in HEMA culture were viable (Table 3). The low viability of ex vivo generated erythroid cells is attributed to the range of apoptosis‐inducing agents released by the cells during culture 42,43 . A similar slight (18%), but not significant (p > 0.5), reduction in number of viable erythroid cells was observed after thawing short‐ and long‐term‐stored cells (Fig.…”

Section: Resultsmentioning

confidence: 70%

TRANSPLANTATION AND CELLULAR ENGINEERING: Long‐term storage does not alter functionality of in vitro generated human erythroblasts: implications for ex vivo generated erythroid transfusion products

Migliaccio

Sanchez²,

LeBlanc³

et al. 2009

Transfusion

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“…The score of this match ranks 2 out of 17254 upstream regions. Tgfb1 is known to be involved in IL-3-dependent early erythropoiesis [52]. Strong matches are also seen for Klf1 (rank 42/17254), a very important transcriptional regulator of erythropoeisis, and for Gata-5 (rank 73/17254), which, like Gata-1, binds GATA DNA sites.…”

Section: Resultsmentioning

confidence: 79%

Discovering Sequence Motifs with Arbitrary Insertions and Deletions

et al. 2008

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Biology is encoded in molecular sequences: deciphering this encoding remains a grand scientific challenge. Functional regions of DNA, RNA, and protein sequences often exhibit characteristic but subtle motifs; thus, computational discovery of motifs in sequences is a fundamental and much-studied problem. However, most current algorithms do not allow for insertions or deletions (indels) within motifs, and the few that do have other limitations. We present a method, GLAM2 (Gapped Local Alignment of Motifs), for discovering motifs allowing indels in a fully general manner, and a companion method GLAM2SCAN for searching sequence databases using such motifs. glam2 is a generalization of the gapless Gibbs sampling algorithm. It re-discovers variable-width protein motifs from the PROSITE database significantly more accurately than the alternative methods PRATT and SAM-T2K. Furthermore, it usefully refines protein motifs from the ELM database: in some cases, the refined motifs make orders of magnitude fewer overpredictions than the original ELM regular expressions. GLAM2 performs respectably on the BAliBASE multiple alignment benchmark, and may be superior to leading multiple alignment methods for “motif-like” alignments with N- and C-terminal extensions. Finally, we demonstrate the use of GLAM2 to discover protein kinase substrate motifs and a gapped DNA motif for the LIM-only transcriptional regulatory complex: using GLAM2SCAN, we identify promising targets for the latter. GLAM2 is especially promising for short protein motifs, and it should improve our ability to identify the protein cleavage sites, interaction sites, post-translational modification attachment sites, etc., that underlie much of biology. It may be equally useful for arbitrarily gapped motifs in DNA and RNA, although fewer examples of such motifs are known at present. GLAM2 is public domain software, available for download at http://bioinformatics.org.au/glam2.

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IL‐3‐Dependent Early Erythropoiesis Is Stimulated by Autocrine Transforming Growth Factor Beta

Cited by 17 publications

References 33 publications

Prospective isolation of human erythroid lineage-committed progenitors

Prospective isolation of human erythroid lineage-committed progenitors

TRANSPLANTATION AND CELLULAR ENGINEERING: Long‐term storage does not alter functionality of in vitro generated human erythroblasts: implications for ex vivo generated erythroid transfusion products

Discovering Sequence Motifs with Arbitrary Insertions and Deletions

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