2020
DOI: 10.1101/2020.02.26.960443
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IL-6 signalling protects zebrafish larvae duringStaphylococcus epidermidisinfection in a novel bath immersion model

Abstract: The host immune responses to Staphylococcus epidermidis, a frequent cause of nosocomial infections, is not well understood. We have established a novel bath immersion model of this infection in zebrafish larvae. Macrophages play a primary role in the host immune response and are involved in clearance of infection in the larvae. S.epidermidis infection activates Tlr-2 signalling pathway by upregulation of tlr-2. There is a marked inflammation characterised by heightened NF-B signalling and elevation of several… Show more

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Cited by 3 publications
(2 citation statements)
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“…mir-142a-3p was one of 21 differentially expressed miRNAs between grass carp susceptible and resistant to A. hydrophila (23,51); the findings were consistent with a previous report that mir-142a-5p was involved in the regulation of the IL-6 signaling pathway, thereby modulating inflammation (52). In the present study, we focused on the function of miR-142a-3p in the inflammatory response of grass carp infected with A. hydrophila.…”
Section: Discussionsupporting
confidence: 91%
“…mir-142a-3p was one of 21 differentially expressed miRNAs between grass carp susceptible and resistant to A. hydrophila (23,51); the findings were consistent with a previous report that mir-142a-5p was involved in the regulation of the IL-6 signaling pathway, thereby modulating inflammation (52). In the present study, we focused on the function of miR-142a-3p in the inflammatory response of grass carp infected with A. hydrophila.…”
Section: Discussionsupporting
confidence: 91%
“…Survival of zebrafish through 72 hpi remained at 100% following infection in 1×10 7 spirochetes/mL B. burgdorferi suspension (Table 1), indicating no toxicity from either the immersion suspension media (BSK-H) or from B. burgdorferi . Although suspension of zebrafish in pure water or PBS would tailor to the needs of the fish [34,35,36,37], B. burgdorferi have been found to form round bodies within 10 minutes of exposure to pure distilled water [38]. By diluting BSK-H media 1:1 with zebrafish water, the spirochete morphology of B. burgdorferi was preserved throughout the 8-hour infection period (verified via darkfield microscopy of the suspensions), indicating that B. burgdorferi remained in their active, motile state.…”
Section: Discussionmentioning
confidence: 99%