IL-8 concentration in coronary sinus blood during early coronary reperfusion after ischemic arrest

Lango, Romuald; Anisimowicz, Lech; Siebert, Janusz; Rogowski, Jan; Bakowska, Alicja; Mroziński, Paweł; Narkiewicz, M

doi:10.1016/s1010-7940(01)00846-6

Cited by 6 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ischemic inflammatory myocardial injury began during the period in which the blood supply was interrupted and increased during the period of coronary reperfusion 40 , 41 , along with a greater expression of COX-2 34 mRNA.…”

Section: Discussionmentioning

confidence: 99%

Selective Cyclooxygenase-2 Inhibition Protects Against Myocardial Damage in Experimental Acute Ischemia

Carnieto

Dourado

Luz

et al. 2009

Clinics

View full text Add to dashboard Cite

Carnieto Jr. A, Dourado PMM, da Luz PL, Chagas ACP. Selective cyclooxygenase-2 inhibition protects against myocardial damage in experimental acute ischemia. Clinics. 2009;64(3):245-52. BACKGROUND:Acute myocardial infarction is associated with tissue inflammation. Early coronary reperfusion clearly improves the outcome but may help propagate the inflammatory response and enhance tissue damage. Cyclooxygenase-2 is an enzyme that catalyzes the initial step in the formation of inflammatory prostaglandins from arachidonic acid. Cyclooxygenase-2 levels are increased when ischemic cardiac events occur. The overall function of COX-2 in the inflammatory process generated by myocardial ischemic damage has not yet been elucidated. GOAL: The objective of this study was to determine whether a selective cyclooxygenase-2 inhibitor (rofecoxib) could alter the evolution of acute myocardial infarction after reperfusion. METHODS AND RESULTS:This study was performed with 48 mongrel dogs divided into two groups: controls and those treated with the drug. All animals were prepared for left anterior descending coronary artery occlusion. The dogs then underwent 180 minutes of coronary occlusion, followed by 30 minutes of reperfusion. Blood samples were collected from the venous sinus immediately before coronary occlusion and after 30 minutes of reperfusion for measurements of CPK-MB, CPK-MBm and troponin I. During the experiment we observed the mean blood pressure, heart rate and coronary flow. The coronary flow and heart rate did not change, but in the control group, there was blood pressure instability, in addition to maximal levels of CPK-MB post-infarction. The same results were observed for CPK-MBm and troponin I. CONCLUSION: In a canine model of myocardial ischemia-reperfusion, selective inhibition of Cyclooxygenase-2 with rofecoxib was not associated with early detrimental effects on the hemodynamic profile or the gross extent of infarction; in fact, it may be beneficial by limiting cell necrosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Selective Cyclooxygenase-2 Inhibition Protects Against Myocardial Damage in Experimental Acute Ischemia

Carnieto

Dourado

Luz

et al. 2009

Clinics

View full text Add to dashboard Cite

show abstract

“…The selective increase in IL-8 following CS with or without CPB thus requires further attention. Several studies have focused on IL-8 synthesis in coronary sinus blood or arterial blood samples after CS with or without CPB [11,12]. Due to different findings, it remained unclear whether the myocardium or the lungs were the main source of IL-8 secretion.…”

Section: Discussionmentioning

confidence: 99%

Pro-inflammatory cytokines after different kinds of cardio-thoracic surgical procedures: is what we see what we know?

Franke¹,

Lante²,

Fackeldey³

et al. 2005

European Journal of Cardio-Thoracic Surgery

151

117

View full text Add to dashboard Cite

Surgical trauma and reperfusion injury appear to represent the predominant factors resulting in immunologic changes after cardiac surgery. Cardiopulmonary bypass (CPB) may be less important for immune response and acute-phase reactions than previously suspected. In addition, our data indicate a relationship between IL-6 synthesis and the degree of surgical trauma. IL-8 appears to be elevated only after cardiac surgery whereas PCT liberation depended on the use of ECC.

show abstract

“…Likewise, the portion of cells maintaining the phenotype of unstimulated neutrophils remained constant at approximately 10%-20% between 300 pM and 10 nM of Gro-a. At concentrations of IL-8 exceeding its median plasma concentration range in healthy individuals of approximately 0.2-0.5 pM, [19][20][21] virtually identical stepwise phenotypic conversions of neutrophils were elicited as observed above for Gro-a (images not shown).…”

Section: Chemokine-induced F-actin Formation In Primary Human Neutropmentioning

confidence: 64%

Phenotypic Analysis of Chemokine-Driven Actin Reorganization in Primary Human Neutrophils

Kredel

Wolff

Gierschik

et al. 2014

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

The chemokine-driven activation of CXC-type chemokine receptors 1/2 (CXCR1/2) and the subsequent reorganization of the neutrophilic actin are early key events in the induction of neutrophil migration toward centers of inflammation. In this study, an image analysis algorithm was developed to detect subtle chemokine-induced changes in the actin cytoskeleton of primary human neutrophils. By this means, a discrete early step of neutrophil activation was dissected that could be initiated by concentrations of growth-related oncogen α (Gro-α) or interleukin-8 (IL-8) just above their resting-state plasma levels. The associated half-maximal effective concentration (EC50) values for Gro-α and IL-8 of 8 and 22 pM, respectively, are between two and three orders of magnitude below the so-far reported EC50 values of these chemokines for the induction of neutrophilic calcium release, integrin expression, degranulation, and receptor internalization. Sch527123, a known inhibitor of CXCR2 (KD=49 pM) and with a lower potency/affinity also of CXCR1 (KD=3.9 nM), antagonized actin remodeling with half-maximal inhibitory concentration (IC50) values of 400 pM for the CXCR2-specific agonist Gro-α and of 36 nM for the CXCR1/2-promiscuous agonist IL-8. This observation indicates that the here-described early step of chemokine-driven actin reorganization is modulated by both CXCR1 and CXCR2. Thus, the imaging-based assay format, as developed in this work, may be employed in a phenotypic screening campaign to identify inhibitors of an early step in CXCR1/2-induced neutrophilic chemotaxis.

show abstract

IL-8 concentration in coronary sinus blood during early coronary reperfusion after ischemic arrest

Abstract: We conclude that the heart is not the main source of IL-8 in early coronary reperfusion, although coronary reperfusion induces its release.

Cited by 6 publications

References 25 publications

Selective Cyclooxygenase-2 Inhibition Protects Against Myocardial Damage in Experimental Acute Ischemia

Selective Cyclooxygenase-2 Inhibition Protects Against Myocardial Damage in Experimental Acute Ischemia

Pro-inflammatory cytokines after different kinds of cardio-thoracic surgical procedures: is what we see what we know?

Phenotypic Analysis of Chemokine-Driven Actin Reorganization in Primary Human Neutrophils

Contact Info

Product

Resources

About