IL1R2 promotes tumor progression via JAK2/STAT3 pathway in human clear cell renal cell carcinoma

Liu, Yingting; Xing, Zhaoyu; Yuan, Maoling; Xu, Bin; Chen, Lüjun; Zhang, Dachuan; Zhou, You; Huang, Hao; Zheng, Xiao; Zhang, Jinping; Jiang, Jingting

doi:10.1016/j.prp.2022.154069

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…sion had the opposite effect. 28 Little evidence has been dedicated to study the role of IL1R2 in ARDS pathogenesis at the level of mechanism. Higher plasma levels of IL-1R2 were associated with increased mortality in ARDS patients.…”

Section: Discussionmentioning

confidence: 99%

“…IL1R2 expression increased with a higher tumor grade of clear cell renal cell carcinoma (ccRCC) and was associated with a worse overall survival rate. Depletion of IL1R2 inhibited cell proliferation, migration, and invasion, while overexpression had the opposite effect 28 . Little evidence has been dedicated to study the role of IL1R2 in ARDS pathogenesis at the level of mechanism.…”

Section: Discussionmentioning

confidence: 99%

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RETRACTED: Development of the ARDS‐derived gene panel for lung adenocarcinoma prognosis stratification and experiment validation of CCL20 expression

Fang,

Shi,

Huang

et al. 2024

Environmental Toxicology

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Acute respiratory distress syndrome (ARDS) is a life‐threatening condition characterized by lung inflammation and high mortality rates. Lung cancer, specifically lung adenocarcinoma (LUAD), is a major cause of cancer‐related deaths worldwide. Patients with LUAD, particularly those undergoing chemotherapy, are more likely to develop ARDS. ARDS inflicts major malfunctioning in the immune system. We suspected a certain shared pathogenic mechanism between these diseases. This study analyzed 503 LUAD patients from the TCGA‐LUAD cohort as the training set, 85 LUAD cases from the GSE30219 cohort as the validation set, and 24 RNA‐seq samples from ARDS mice model and control groups in the GSE2411 cohort. The differentially expressed genes (DEGs) of ARDS were analyzed using the limma package and screened by Cox and Lasso analysis. ssGSEA and xCell algorithms were utilized for immune landscaping. RT‐qPCR analysis was used to determine the mRNA levels of key genes in both the LPS‐induced ARDS model and human LUAD cell lines. We identified DEGs between ARDS and control groups, which were highly associated with cytokine production and leukocyte migration. A prognosis model for LUAD patients was developed based on the expressions of the key genes in the ARDS‐derived DEGs, including FMO3, IL1R2, CCL20, CFTR, and GADD45G. A satisfactory efficacy was observed in both the training and validation cohorts. The model demonstrated increased effectiveness in predicting the intratumor immune profile and mutation status of LUAD. Moreover, we utilized LPS to induce the ARDS model, which resulted in elevated expressions of IL1R2 and CCL20. Additionally, CCL20 was upregulated in cancerous LUAD cell lines. We developed an ARDS‐based model for stratifying LUAD prognosis. CCL20 was found to be elevated in both the ARDS model and LUAD, suggesting a shared underlying mechanism of these two diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

RETRACTED: Development of the ARDS‐derived gene panel for lung adenocarcinoma prognosis stratification and experiment validation of CCL20 expression

Fang,

Shi,

Huang

et al. 2024

Environmental Toxicology

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“…However, a more specific mechanism needs to be explored. JAK/STAT3 pathways in KIRC, which was the most studied pathway of them [26][27][28]. Furthermore, in this study, although we referred to and analyzed a large number of clinical samples of KIRC in public databases, and selected TROAP as the research target based on this, we did not collect clinical samples of KIRC patients for testing in subsequent studies, which may need to be gradually improved in future studies.…”

Section: Discussionmentioning

confidence: 99%

TROAP Promotes the Proliferation, Migration, and Metastasis of Kidney Renal Clear Cell Carcinoma with the Help of STAT3

Wang

Wan

et al. 2023

IJMS

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Kidney renal clear cell carcinoma (KIRC) is a subtype of renal cell carcinoma that threatens human health. The mechanism by which the trophinin-associated protein (TROAP)–an important oncogenic factor–functions in KIRC has not been studied. This study investigated the specific mechanism by which TROAP functions in KIRC. TROAP expression in KIRC was analyzed using the RNAseq dataset from the Cancer Genome Atlas (TCGA) online database. The Mann–Whitney U test was used to analyze the expression of this gene from clinical data. The Kaplan–Meier method was used for the survival analysis of KIRC. The expression level of TROAP mRNA in the cells was detected using qRT-PCR. The proliferation, migration, apoptosis, and cell cycle of KIRC were detected using Celigo, MTT, wound healing, cell invasion assay, and flow cytometry. A mouse subcutaneous xenograft experiment was designed to demonstrate the effect of TROAP expression on KIRC growth in vivo. To further investigate the regulatory mechanism of TROAP, we performed co-immunoprecipitation (CO-IP) and shotgun liquid chromatography–tandem mass spectrometry (LC-MS). TCGA-related bioinformatics analysis showed that TROAP was significantly overexpressed in KIRC tissues and was related to higher T and pathological stages, and a poor prognosis. The inhibition of TROAP expression significantly reduced the proliferation of KIRC, affected the cell cycle, promoted cell apoptosis, and reduced cell migration and invasion. The subcutaneous xenograft experiments showed that the size and weight of the tumors in mice were significantly reduced after TROAP-knockdown. CO-IP and post-mass spectrometry bioinformatics analyses revealed that TROAP may combine with signal transducer and activator of transcription 3 (STAT3) to achieve tumor progression in KIRC; this was verified by functional recovery experiments. TROAP may regulate KIRC proliferation, migration, and metastasis by binding to STAT3.

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“…The balance between IL1R2 and other interleukin-1 receptors is essential to maintain an appropriate immune response and prevent excessive inflammatory responses. [18] Studies have shown that IL1R2 and eastern China the occurrence of sporadic Parkinson disease. [19] IL1R1 and IL1R2 polymorphisms associated with the risk of tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

Il1r2 and Tnfrsf12a in transcranial magnetic stimulation effect of ischemic stroke via bioinformatics analysis

Zhao,

Liu,

et al. 2024

Medicine

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Ischemic stroke refers to ischemic necrosis or softening of localized brain tissue. Transcranial magnetic stimulation (TMS) is a painless, noninvasive and green treatment method, which acts on the central nervous system through a pulsed magnetic field to assist in the treatment of central nervous system injury diseases. However, the role of Il1r2 and Tnfrsf12a in this is unknown. The ischemic stroke datasets GSE81302 and TMS datasets GSE230148 were downloaded from Gene Expression Omnibus database. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis (WGCNA) was performed. The construction and analysis of protein-protein interaction (PPI) network and functional enrichment analysis were performed. Draw heat map gene expression. Through the Comparative Toxicogenomics Database (CTD) to find the most relevant and core gene diseases. TargetScan was used to screen miRNAs regulating DEGs. A total of 39 DEGs were identified. According to gene ontology (GO) analysis results, in biological process (BP) analysis, they were mainly enriched in the positive regulation of apoptosis process, inflammatory response, positive regulation of p38MAPK cascade, and regulation of cell cycle. In cellular component (CC) analysis, they were mainly enriched in the cell surface, cytoplasm, and extracellular space. In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, they were mainly enriched in nf-κB signaling pathway, fluid shear stress and atherosclerosis, P53 signaling pathway, TNF signaling pathway, and apoptosis. Among the enrichment items of metascape, negative regulation of T cell activation, hematopoietic cell lineage, positive regulation of apoptotic process, fluid shear stress and atherosclerosis were observed in GO enrichment items. Five core genes (Socs3, Irf1, Il1r2, Ccr1, and Tnfrsf12a) were obtained, which were highly expressed in ischemic stroke samples. Il1r2 and Tnfrsf12a were lowly expressed in TMS samples. CTD analysis found that the core gene (Socs3, Irf1 and Il1r2, Ccr1, Tnfrsf12a) and ischemic stroke, atherosclerosis, hypertension, hyperlipidemia, thrombosis, stroke, myocardial ischemia, myocardial infarction, and inflammation. Il1r2 and Tnfrsf12a are highly expressed in ischemic stroke, but lowly expressed in TMS samples.

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IL1R2 promotes tumor progression via JAK2/STAT3 pathway in human clear cell renal cell carcinoma

Cited by 8 publications

References 37 publications

RETRACTED: Development of the ARDS‐derived gene panel for lung adenocarcinoma prognosis stratification and experiment validation of CCL20 expression

RETRACTED: Development of the ARDS‐derived gene panel for lung adenocarcinoma prognosis stratification and experiment validation of CCL20 expression

TROAP Promotes the Proliferation, Migration, and Metastasis of Kidney Renal Clear Cell Carcinoma with the Help of STAT3

Il1r2 and Tnfrsf12a in transcranial magnetic stimulation effect of ischemic stroke via bioinformatics analysis

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