IL1β/ TNFα/COX-2/VEGF axis responsible for effective healing potential of C-glucoside xanthone (mangiferin) based ointment in immunocompromised rats

Yadav, Virendra Pratap; Shukla, Aditya; Choudhury, Soumen; Singh, Rajneesh; Anand, Mukul; Prabhu, Shyama N.

doi:10.1016/j.cyto.2022.156012

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…Overall, the wound-healing capabilities of SMPE compared with standard mangiferin were proven through mechanisms involving fibroblast proliferation and migration. Our results confirmed the previous pharmacological activity evaluations of mangiferin and mango peel extract for wound healing and the treatment of skin diseases [10,12,26,27]. Moreover, SMPE exhibited nontoxic effects across the tested concentrations (0.25-10 µg/mL) and contributed to fibroblast proliferation at the optimal concentration of 1 µg/mL.…”

Section: Bioactivities Of Standardized Mangiferin-rich Manaifera Indi...supporting

confidence: 90%

“…The migration rate of the control group was notably lower than that of cells treated with SMPE and standard mangiferin (Figure 6). The results align with other studies suggesting that mangiferin can enhance wound-healing activity [26,27]. Moreover, the synthesis of type I collagen was assessed in HDFs after 48 h of exposure to mangiferin or SMPE at concentrations ranging from 0.25 to 10 µg/mL (Table 5).…”

Section: Bioactivities Of Standardized Mangiferin-rich Manaifera Indi...supporting

confidence: 88%

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Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Sumpavapol,

Waehayee,

Suklim

et al. 2023

Sustainability

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The light mango or “Ma-Muang Bao” (Mangifere indica L. var.) is a native mango species originating from Malaysia and southern Thailand. The whole Ma-Muang Bao fruit, except peels, is popular as both a raw and ripe fruit for consumption, as well as being used in various processed food products. This study aims to transform the peel of a specific mango variety, which is a byproduct of the agro-industrial sweet, pickled mangoes industry, into a valuable bioactive ingredient for healthcare products. This is achieved through the establishment of a standardized mangiferin-rich mango peel extract (SMPE). Employing the Box–Behnken design (BBD) within the framework of response surface methodology (RSM), an optimal microwave-assisted extraction procedure was developed. A total of 27 experiments, each with four independent variables, including solvent ratio, extraction power, extraction time, and ethanol (EtOH) ratio, were conducted to optimize the extraction method in terms of mangiferin content and extraction yield. The optimized extraction conditions encompassed a solvent ratio of 120 mL EtOH/100 g sample, an extraction power of 450 W, an extraction time of approximately 4.3 min, and an EtOH ratio of 69.44% (EtOH in water). Small-scale extractions were carried out using the following specified parameters: solvent ratio of 120 mL, extraction power of 450 W, extraction time of 4 min, and EtOH ratio of 70% EtOH. These extractions yielded an extract with a mangiferin content of 27.24 ± 2.05 mg/g and an extraction yield of 3.71 ± 0.17% w/w. Notably, these outcomes were better from the mangiferin content of 19.62 mg/g and a yield of fresh peel of 5.61% estimated through BBD analysis. Furthermore, a pilot-scale extraction was performed using 7 kg of fresh mango peel and 70% EtOH (8.4 L) for 4 min, resulting in an extract with a mangiferin content of 51.85 ± 0.35 mg/g and a fresh peel yield of 4.35% w/w. This method emerges as the most suitable for mango peel extraction and forms the basis of the SMPE. The results from biological activities highlight the potential use of SMPE as the active ingredient for cosmeceutical or healthcare products for wound-healing and skin-brightening agents. Additionally, the knowledge from this study presents an alternative approach to various plant sources and sustainable extraction methods for the herbal extract industry.

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Section: Bioactivities Of Standardized Mangiferin-rich Manaifera Indi...supporting

confidence: 90%

Section: Bioactivities Of Standardized Mangiferin-rich Manaifera Indi...supporting

confidence: 88%

Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Sumpavapol,

Waehayee,

Suklim

et al. 2023

Sustainability

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“…The COX–VEGF axis plays an important role in wound healing, since pharmacologically induced downregulation of COX-2 correlates with significantly increased VEGF levels. A study of full-thickness skin incisions in mice demonstrated increased angiogenesis and collagen deposition on histology, which correlated with a significant increase in wound contraction and reduced wound area macroscopically [ 47 ]. These findings are in contrast to those of another paper included in our analysis, indicating that COX-2 KO mice demonstrate reduced VEGF-induced angiogenesis at the anastomosis [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Knockout Genes in Bowel Anastomoses: A Systematic Review of Literature Outcomes

Geropoulos,

Psarras,

Koimtzis

et al. 2024

JPM

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Background: The intestinal wound healing process is a complex event of three overlapping phases: exudative, proliferative, and remodeling. Although some mechanisms have been extensively described, the intestinal healing process is still not fully understood. There are some similarities but also some differences compared to other tissues. The aim of this systematic review was to summarize all studies with knockout (KO) experimental models in bowel anastomoses, underline any recent knowledge, and clarify further the cellular and molecular mechanisms of the intestinal healing process. A systematic review protocol was performed. Materials and methods: Medline, EMBASE, and Scopus were comprehensively searched. Results: a total of eight studies were included. The silenced genes included interleukin-10, the four-and-one-half LIM domain-containing protein 2 (FHL2), cyclooxygenase-2 (COX-2), annexin A1 (ANXA-1), thrombin-activatable fibrinolysis inhibitor (TAFI), and heparin-binding epidermal growth factor (HB-EGF) gene. Surgically, an end-to-end bowel anastomosis was performed in the majority of the studies. Increased inflammatory cell infiltration in the anastomotic site was found in IL-10-, annexin-A1-, and TAFI-deficient mice compared to controls. COX-1 deficiency showed decreased angiogenesis at the anastomotic site. Administration of prostaglandin E2 in COX-2-deficient mice partially improved anastomotic leak rates, while treatment of ANXA1 KO mice with Ac2-26 nanoparticles reduced colitis activity and increased weight recovery following surgery. Conclusions: our findings provide new insights into improving intestinal wound healing by amplifying the aforementioned genes using appropriate gene therapies. Further research is required to clarify further the cellular and micromolecular mechanisms of intestinal healing.

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“…As these factors are implicated in the development of gastric ulcers, MPE may facilitate wound healing in peptic ulcers by reducing them. According to the findings of several studies, mangiferin has been shown to facilitate wound closure in excision or incision wounds through the downregulation of COX-2, as well as by stimulating angiogenesis by increasing the secretion of the vascular endothelial growth factor (VEGF) [54][55][56]. This suggests that MPE may have a multifaceted mechanism of action, making it a potentially useful treatment option for promoting the healing of peptic ulcers.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Biological Activities of Mahajanaka Mango Pulp Extract in Murine Models

Paradee,

Janthip,

Taesothikul

et al. 2023

Applied Sciences

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Mahajanaka mango, a hybrid cultivar of Mangifera indica Linn., is a highly nutritional fruit that is popularly consumed in Thailand. It has been used in traditional medicine due to its abundance of phytonutrients. The present study aimed to investigate the chemical compositions and antioxidant activity of Mahajanaka mango pulp extract (MPE) in vitro. Additionally, we examined its biological activities, including the analgesic, anti-inflammatory, gastroprotective, and hepatoprotective effects of MPE, in murine models. MPE exhibited high levels of phenolic compounds, mangiferin, β-carotene, and vitamin C, and it potentially showed antioxidant properties in an ABTS scavenging assay. The animal results have revealed that oral administration of MPE (1000 mg/kg body weight (BW)) significantly decreased acetic acid-induced writhing responses in mice. Interestingly, local applications of MPE at 1 mg/ear ameliorated ethyl phenylpropiolate (EPP)-induced ear edema, while gavage of MPE at 1000 mg/kg BW significantly decreased carrageenan-induced hind paw edema in rats. MPE can also protect against gastric ulcers induced by stress, hydrochloric acid/ethanol, and indomethacin in rats. Indeed, MPE (250 mg/kg BW) markedly lowered the level of serum alanine aminotransferase activity and hepatic lipid accumulation in rats with CCl4- and paracetamol-induced hepatotoxicity. Taken together, the findings suggested that MPE exerts potent antioxidant, analgesic, anti-inflammatory, gastroprotective, and hepatoprotective effects.

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IL1β/ TNFα/COX-2/VEGF axis responsible for effective healing potential of C-glucoside xanthone (mangiferin) based ointment in immunocompromised rats

Cited by 5 publications

References 35 publications

Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Box–Behnken Design to Optimize Standardized Mangiferin-Rich Mango Peel Extract from Agro-Industrial Waste Product

Knockout Genes in Bowel Anastomoses: A Systematic Review of Literature Outcomes

Antioxidant and Biological Activities of Mahajanaka Mango Pulp Extract in Murine Models

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