ILB 938, a valuable faba bean (<i>Vicia faba</i> L.) accession

Khazaei, Hamid; Link, Wolfgang; Street, Kenneth; Stoddard, Frederick L.

doi:10.1017/s1479262118000205

Cited by 20 publications

(18 citation statements)

References 42 publications

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“…ILB938/2 showed the lowest stomatal conductance, apparent specific root length, and apparent root length density in both treatments, suggesting that its drought tolerance is based on closed stomata rather than accessing water. Low stomatal conductance in ILB938/2 has previously been interpreted as an indicator of drought tolerance (Khan et al, 2007; Khazaei et al, 2018), but the ability of the plant to regulate stomatal conductance may be a better strategy than continuously low conductance, as shown in common bean ( Phaseolus vulgaris L. cv. “Pinto Saltillo”) (Rosales et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Association of Shoot and Root Responses to Water Deficit in Young Faba Bean (Vicia faba L.) Plants

et al. 2019

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Water deficit may occur at any stage of plant growth, with any intensity and duration. Phenotypic acclimation and the mechanism of adaptation vary with the evolutionary background of germplasm accessions and their stage of growth. Faba bean is considered sensitive to various kinds of drought. Hence, we conducted a greenhouse experiment in rhizotrons under contrasting watering regimes to explore shoot and root traits and drought avoidance mechanisms in young faba bean plants. Eight accessions were investigated for shoot and root morphological and physiological responses in two watering conditions with four replications. Pre-germinated seedlings were transplanted into rhizotron boxes filled with either air-dried or moist peat. The water-limited plants received 50-ml water at transplanting and another 50-ml water 4 days later, then no water was given until the end of the experimental period, 24 days after transplanting. The well-watered plants received 100 ml of water every 12 h throughout the experimental period. Root, stem, and leaf dry mass, their mass fractions, their dry matter contents, apparent specific root length and density, stomatal conductance, SPAD value, and Fv/Fm were recorded. Water deficit resulted in 3–4-fold reductions in shoot biomass, root biomass, and stomatal conductance along with 1.2–1.4-fold increases in leaf and stem dry matter content and SPAD values. Total dry mass and apparent root length density showed accession by treatment interactions. Accessions DS70622, DS11320, and ILB938/2 shared relatively high values of total dry mass and low values of stomatal conductance under water deficit but differed in root distribution parameters. In both treatments, DS70622 was characterized by finer roots that were distributed in both depth and width, whereas DS11320 and ILB938/2 produced less densely growing, thicker roots. French accession Mélodie/2 was susceptible to drought in the vegetative phase, in contrast to previous results from the flowering phase, showing the importance of timing of drought stress on the measured response. Syrian accession DS70622 explored the maximum root volume and maintained its dry matter production, with the difference from the other accessions being particularly large in the water-limited treatment, so it is a valuable source of traits for avoiding transient drought.

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Section: Discussionmentioning

confidence: 99%

Association of Shoot and Root Responses to Water Deficit in Young Faba Bean (Vicia faba L.) Plants

et al. 2019

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“…It is homozygous for the recessive gene zt2, and thus is a zero‐tannin/white flower line. ILB 938/2 (selection from IG 12132, https://doi.org/10.18730/60FD2) is a wild‐type accession (tannin‐containing) with spotted flower from the Andean region of Ecuador and Colombia (Khazaei, Link, Street, & Stoddard, ). The plants of each generation were grown in pollinator‐free environments to avoid outcrossing.…”

Section: Methodsmentioning

confidence: 99%

Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba)

2019

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Faba bean has the potential to become a key food and feed protein crop in many areas of the world. The presence of tannins in its seed coat has limited the deployment of this crop as feed and food. The expression of either of the two recessive genes, zt1and zt2, causes a great reduction of tannins from the seed coat and results in a white flower phenotype. Molecular markers linked to these loci are fundamental tools for speeding up the breeding of low-tannin varieties. The main aim of this study was to develop and validate a robust molecular marker linked to the zt2 locus. We used 176 recombinant inbred lines of the Disco/2 × ILB 938/2 cross at F 6 and genotyped those using 257 SNP (single nucleotide polymorphism) markers. An SNP marker associated with zt2 locus was found on faba bean chromosome 3 and was used to develop a high-throughput low-cost KASP (kompetitive allele-specific PCR) marker. The KASP marker can successfully discriminate low-tannin faba beans carrying zt2 from those carrying zt1 and wild-type alleles. K E Y W O R D S broad bean, candidate gene, flower colour, molecular breeding, SNP marker, tannins S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Zanotto S, Vandenberg A, Khazaei H. Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba). Plant Breed. 2020;139:375-380. https ://doi.

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“…Hence, we hypothesized that V. faba accessions from equatorial highlands (low latitude at high altitude) and sub-boreal lowlands (high latitude at low altitude) would react differently to solar blue and UV light. ILB938, a selection from the Andean region of Colombia and Ecuador, 23 was chosen as the representative from equatorial highlands, and Aurora, an old Swedish cultivar, as the representative from sub-boreal lowlands. The estimated yearly maximum UV-B irradiance is more than 50% higher in the Ecuadorian Andean region than in Southern Sweden (ESI Table 1 †).…”

Section: Introductionmentioning

confidence: 99%

Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments

Yan

Stoddard

Neugart

et al. 2019

Photochem Photobiol Sci

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ILB 938, a valuable faba bean (Vicia faba L.) accession

Cited by 20 publications

References 42 publications

Association of Shoot and Root Responses to Water Deficit in Young Faba Bean (Vicia faba L.) Plants

Association of Shoot and Root Responses to Water Deficit in Young Faba Bean (Vicia faba L.) Plants

Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba)

Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments

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