Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats

Ampting, Marleen T. J. van; Rodenburg, Wendy; Vink, Carolien; Kramer, Evelien; Schonewille, Arjan J.; Keijer, Jaap; Meer, Roelof van der; Bovee‐Oudenhoven, Ingeborg M. J.

doi:10.1007/s10620-008-0685-0

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…This indicated that it is the cleaved form of Reg3b that binds to the pathogens, not the full-length polypeptide. We have shown previously that the Reg3 protein is not precipitated even when centrifuged at 15,000 ϫ g (25). Therefore, the results presented here indicate that ileal mucosal Reg3b is able to directly bind both Salmonella and Listeria.…”

Section: Resultssupporting

confidence: 58%

“…Reg3g and Reg3b expression was determined in the ileum because this is the area where expression is upregulated during infection of rats (22,25) and upon microbial colonization of germ-free mice (3). Frozen mucosal scrapings of the ileum were pulverized under liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…Studies in rodents indicate that innate recognition of bacteria or bacterial components triggers epithelial expression of secreted C-type lectins Reg3g and Reg3b (3,12,22,25). In rodents, mucosal and fecal levels of the Reg3 protein are associated with the severity of infection (25).…”

mentioning

confidence: 99%

“…In rodents, mucosal and fecal levels of the Reg3 protein are associated with the severity of infection (25). The human homologue of this protein (i.e., they are, on the amino acid level, the most identical proteins between the two species [bidirectional best hit]) is pancreatitisassociated protein (PAP).…”

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confidence: 99%

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Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

et al. 2012

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Section: Resultssupporting

confidence: 58%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

et al. 2012

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“…Reg III family genes are likely to act predominantly in the lower GI tract, and, therefore, we cannot support confidently the involvement of Reg I gene in NSAID-induced lower GI lesions. However, because REG family proteins are secreted into the GI tract lumen (26,27), it may be interesting to speculate that REG I protein produced in the upper GI tract may act in the lower GI tract as well.…”

Section: Discussionmentioning

confidence: 99%

Expression ofRegfamily genes in the gastrointestinal tract of mice treated with indomethacin

Sun

Fukui

Hara

et al. 2015

American Journal of Physiology-Gastrointestinal and Liver Physi

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Regenerating gene (Reg) family proteins, which are classified into four types, commonly act as trophic and/or antiapoptotic factors in gastrointestinal (GI) diseases. However, it remains unclear how these proteins coordinate their similar roles under such pathophysiological conditions. Here, we investigated the interrelationships of Reg family gene expression with mucosal cell proliferation and apoptosis in nonsteroidal anti-inflammatory drug (NSAID)-induced GI injury. GI injury was induced by subcutaneous injection of indomethacin into Reg I knockout (KO) and wild-type (WT) mice, and its severity was scored histopathologically. Temporal changes in the expression of Reg family genes, mucosal proliferation, and apoptosis were evaluated throughout the GI tract by real-time RT-PCR, Ki-67 immunoreactivity, and TUNEL assay, respectively. Reg I, Reg III family, and Reg IV were predominantly expressed in the upper, middle, and lower GI mucosa, respectively. Expression of Reg I and Reg III family genes was upregulated in specific portions of the GI tract after indomethacin treatment. Ki-67-positive epithelial cells were significantly decreased in the gastric and small-intestinal mucosa of Reg I KO mice under normal conditions. After treatment with indomethacin, the number of TUNEL-positive cells was significantly greater throughout the GI mucosa in Reg I KO mice than in WT mice. Expression of Reg I was independent of that of other Reg family genes in, not only normal GI tissues, but also indomethacin-induced GI lesions. Members of the Reg gene family show distinct profiles of expression in the GI tract, and Reg I independently plays a role in protecting the GI mucosa against NSAID-induced injury.Reg; nonsteroidal anti-inflammatory drug; proliferation; apoptosis; gastrointestinal mucosa NONSTEROIDAL ANTI-INFLAMMATORY DRUGS (NSAIDs) are commonly prescribed worldwide for patients with arthritis or cardiac and cerebrovascular diseases (16). Upper gastrointestinal (GI) damage is a major adverse effect of NSAID use (16), and, moreover, recent evidence suggests that NSAIDs are responsible for mucosal injury, not only in the upper, but also the lower GI tract (10, 16). Prostaglandin synthesis is considered to play a pivotal role in the mechanism of NSAID-induced GI damage (21); however, its pathophysiology still remains to be elucidated.The regenerating gene (Reg) was originally isolated from rat regenerating pancreatic islet cells, and its human homolog was named REG I␣ (23). Recently, many Reg-related genes have been isolated and shown to constitute a multigene family (types I-IV) (8). We have previously reported that REG I␣ and REG IV are involved in the pathophysiology of GI inflammation (5, 17) and that, moreover, they act as trophic and/or antiapoptotic factors under inflammatory conditions (17, 18). Other investigators have reported that Reg III family genes are overexpressed in inflamed GI mucosa (11,13) and that their products, including REG I␣ and REG IV proteins, may exert trophic and/or antiapoptotic effects on panc...

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Regenerating (Reg) Gene Family

Gupta

2012

Animal Lectins: Form, Function and Clinical Applications

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Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats

Abstract: PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker.

Cited by 14 publications

References 39 publications

Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice

Expression ofRegfamily genes in the gastrointestinal tract of mice treated with indomethacin

Regenerating (Reg) Gene Family

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