Illuminating Diverse Concomitant DNA Logic Gates and Concatenated Circuits with Hairpin DNA‐Templated Silver Nanoclusters as Universal Dual‐Output Generators

Abstract

issues which should be taken seriously. Plenty of logic devices with multi-output signals are implemented by the aid of covalently labeled reporters (e.g., dye or redox), which cause time wastage and high cost. [10][11][12][13][14][15][16][17] In order to generate more than one output signal, it is ineluctable to introduce many other types of substances (e.g., different dyes and unstable hydrogen peroxide), [18,19] which increase complexity of a logic platform and are much detrimental to the repeatability and assembly of different logic systems. In addition, sequence redesign is required for construction of different logic gates, which leads to high cost and low efficiency. Hence, to explore a simple and noncovalent logic platform in a cost-effective manner is urgently demanded.Aimed at surmounting these drawbacks, new DNA-based logic systems should be considered. DNA-templated silver nanoclusters (AgNCs) with excellent fluorescence properties have attracted wide research interests in the field of fabrication of logic devices, [20][21][22][23][24] due to remarkable merits, such as low cost, tunable emission wavelength, ease of preparation, and convenience of utilization in DNA reaction circuits, and noncovalent (so called label-free) and distinctive logic systems could be designed. [21,22,25,26] Recently, a concept of "DNA Janus Logic Pair", also named as "Contrary Logic Pair", was proposed, [18,27] in which two contrary logic gates (positive and negative gates) were obtained via the same one-time reaction, resulting in significant improvement of computing efficiency. However, systematic complexity remains as a constraining factor for effective implementation and integration of DNA-based logic systems. In this study, diverse concomitant contrary logic gates (CCLGs), such as INHIBIT (INH)^IMPLICATION (IMP), XOR^XNOR and MAJORITY (MAJ)^MINORITY (MIN) ("^" stands for "concomitant and contrary"), and extended concatenated logic circuits (e.g., 4-input MAJ||MIN-INH||IMP, "||" stands for "parallel") with specific functions (e.g., parity generator (Pg) and checker (Pc)) were constructed, by utilization of one novel type of hairpin DNA-templated AgNCs (H-AgNCs) as the universal dualfluorescence signal transducer, and based on the mechanism of DNA hybridization and toehold-mediated strand displacement (TSD). Each pair of CCLGs was achieved via the same DNA (YES^NOT, OR^NOR, INHIBIT^IMPLICATION, XOR^XNOR, and MAJORITY^MINORITY) and extended concatenated logic circuits are presented and some of them perform specific functions, such as parity generators and checkers. The introduction of H-AgNCs as noncovalent signal reporters avoids tedious and high-cost labeling procedures. Of note, the concomitant feature of CCLGs attributed to the dual-emitter AgNCs conduces to reducing the time and cost to devise multiple logic gates. As compared to previous ones, this design eliminates numerous substances (e.g., organic dyes) and unstable components (hydrogen peroxide), which not only decreases the complexity of logic performs and impr...

Micro RNA Sensing with Green Emitting Silver Nanoclusters

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DNA‐Based Adaptive Plasmonic Logic Gates

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