IMA genome - F14

Nest, Magriet A. van der; Chávez, Renato; Vos, Lieschen De; Duong, Tuan A.; Gil-Durán, Carlos; Ferreira, M. A.; Lane, Frances A.; Levicán, Gloria; Santana, Quentin C.; Steenkamp, Emma Theodora; Suzuki, Hiroyuki; Tello, Mario; Rakoma, Jostina R.; Vaca, Inmaculada; Valdés, Natalia; Wilken, P. Markus; Wingfield, Michael J.; Wingfield, Brenda D.

doi:10.1186/s43008-021-00055-1

Cited by 6 publications

(6 citation statements)

References 77 publications

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“…When compared to other species of Chrysoporthe , namely C. austroafricana (44.67 Mb, 13,484 gene models), C. cubensis (42.62 Mb, 13,121 gene models), C. deuterocubensis (43.97 Mb, 13,772 gene models), and C. puriensis (44.66 Mb, 13,166 gene models), the genome size and predicted gene models of C. zambiensis were slightly larger, while the genome size and predicted gene models for C. syzygiicola were slightly smaller [ 42 , 43 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

“…Maximum-likelihood gene trees of the mating-type genes were generated using IQ-TREE v. 1.6.12 [ 35 ], using the built-in selection of the best evolutionary model. These analyses included mating-type gene sequences of C. puriensis [ 16 , 42 ], C. austroafricana [ 43 ], C. cubensis , and C. deuterocubensis [ 44 ], with Cry. parasitica [ 22 ] as an outgroup taxon.…”

Section: Methodsmentioning

confidence: 99%

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The Unique Homothallic Mating-Type Loci of the Fungal Tree Pathogens Chrysoporthe syzygiicola and Chrysoporthe zambiensis from Africa

van der Merwe,

Phakalatsane,

Wilken

2023

Genes

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Chrysoporthe syzygiicola and C. zambiensis are ascomycete tree pathogens first described from Zambia, causing stem canker on Syzygium guineense and Eucalyptus grandis, respectively. The taxonomic descriptions of these two species were based on their anamorphic states, as no sexual states are known. The main purpose of this work was to use whole genome sequences to identify and define the mating-type (MAT1) loci of these two species. The unique MAT1 loci for C. zambiensis and C. syzygiicola consist of the MAT1-1-1, MAT1-1-2, and MAT1-2-1 genes, but the MAT1-1-3 gene is absent. Genes canonically associated with opposite mating types were present at the single mating-type locus, suggesting that C. zambiensis and C. syzygiicola have homothallic mating systems.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Unique Homothallic Mating-Type Loci of the Fungal Tree Pathogens Chrysoporthe syzygiicola and Chrysoporthe zambiensis from Africa

van der Merwe,

Phakalatsane,

Wilken

2023

Genes

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“…This is also true for P. roqueforti. Recent analyses of several genomes from P. roqueforti revealed that depending on the strain, they contain between 34-37 biosynthetic gene clusters [10,109]. As it has been detailed in this review, to date, only six of these biosynthetic gene clusters have been experimentally linked to secondary metabolites produced by P. roqueforti.…”

Section: Future Challenges and Perspectives In The Study Of Secondary...mentioning

confidence: 96%

Secondary Metabolites Produced by the Blue-Cheese Ripening Mold Penicillium roqueforti; Biosynthesis and Regulation Mechanisms

Chávez

Vaca

Estrada

2023

JoF

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Filamentous fungi are an important source of natural products. The mold Penicillium roqueforti, which is well-known for being responsible for the characteristic texture, blue-green spots, and aroma of the so-called blue-veined cheeses (French Bleu, Roquefort, Gorgonzola, Stilton, Cabrales, and Valdeón, among others), is able to synthesize different secondary metabolites, including andrastins and mycophenolic acid, as well as several mycotoxins, such as Roquefortines C and D, PR-toxin and eremofortins, Isofumigaclavines A and B, festuclavine, and Annullatins D and F. This review provides a detailed description of the biosynthetic gene clusters and pathways of the main secondary metabolites produced by P. roqueforti, as well as an overview of the regulatory mechanisms controlling secondary metabolism in this filamentous fungus.

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“…The laeA gene from the closely related species P. rubens (GenBank accession EU685842; [30]) was used to search for the laeA gene in the genome of P. roqueforti CECT 2905 [47] by BLASTN and BLASTX. The gene found and its deduced protein were named PrlaeA and PrLaeA, respectively (see Section 3).…”

Section: Search For the Prlaea Gene In P Roqueforti Cect 2905 And Seq...mentioning

confidence: 99%

“…To identify the PrlaeA gene in P. roqueforti CECT 2905, the laeA gene sequence from P. rubens [30] was utilized to perform BLASTN and BLASTX scans of the P. roqueforti CECT 2905 genome [47]. The search led to the identification of the PrlaeA gene, which is located on contig MSQC01000647.1 and spans nucleotides 44,522 to 45,800.…”

Section: Analysis Of the Prlaea Gene And Its Deduced Protein From P R...mentioning

confidence: 99%

PrlaeA Affects the Production of Roquefortine C, Mycophenolic Acid, and Andrastin A in Penicillium roqueforti, but It Has Little Impact on Asexual Development

Marcano,

Montanares,

Gil-Durán

et al. 2023

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The regulation of fungal specialized metabolism is a complex process involving various regulators. Among these regulators, LaeA, a methyltransferase protein originally discovered in Aspergillus spp., plays a crucial role. Although the role of LaeA in specialized metabolism has been studied in different fungi, its function in Penicillium roqueforti remains unknown. In this study, we employed CRISPR-Cas9 technology to disrupt the laeA gene in P. roqueforti (PrlaeA) aiming to investigate its impact on the production of the specialized metabolites roquefortine C, mycophenolic acid, and andrastin A, as well as on asexual development, because they are processes that occur in the same temporal stages within the physiology of the fungus. Our results demonstrate a substantial reduction in the production of the three metabolites upon disruption of PrlaeA, suggesting a positive regulatory role of LaeA in their biosynthesis. These findings were further supported by qRT-PCR analysis, which revealed significant downregulation in the expression of genes associated with the biosynthetic gene clusters (BGCs) responsible for producing roquefortine C, mycophenolic acid, and andrastin A in the ΔPrlaeA strains compared with the wild-type P. roqueforti. Regarding asexual development, the disruption of PrlaeA led to a slight decrease in colony growth rate, while conidiation and conidial germination remained unaffected. Taken together, our results suggest that LaeA positively regulates the expression of the analyzed BGCs and the production of their corresponding metabolites in P. roqueforti, but it has little impact on asexual development.

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IMA genome - F14

Cited by 6 publications

References 77 publications

The Unique Homothallic Mating-Type Loci of the Fungal Tree Pathogens Chrysoporthe syzygiicola and Chrysoporthe zambiensis from Africa

The Unique Homothallic Mating-Type Loci of the Fungal Tree Pathogens Chrysoporthe syzygiicola and Chrysoporthe zambiensis from Africa

Secondary Metabolites Produced by the Blue-Cheese Ripening Mold Penicillium roqueforti; Biosynthesis and Regulation Mechanisms

PrlaeA Affects the Production of Roquefortine C, Mycophenolic Acid, and Andrastin A in Penicillium roqueforti, but It Has Little Impact on Asexual Development

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