Image processing method for automatic measurement of number of DNA breaks

Abstract

The number of double-strand breaks can be evaluated from the change of average DNA length. The average DNA length is measured by the single-molecule observation method using fluorescence microscope. The measurement of DNA length in the microscope images is done manually by experienced operators and it is time consuming in many experiments. An image processing method using OpenCV library to measure length of DNA in fluorescence microscope images is developed in this paper. An automation of measurement using deep learning is also proposed.