In order to effectively remove the mixed noise, a novel algorithm for image reducing noise combined Pulse Coupled Neural Networks (PCNN) and regularization of Perona-Malik equation (P-M equation) is put forward. Firstly, the positions of impulse noise pollution are located by using PCNN, and the first stage adaptive noise filtering is applied according to the result of noise detection. Secondly, the multi-direction information median filtering is applied according to the local neighborhood noisy information. And then the Gaussian noise in the image is denoised by utilizing the regularization P-M diffusion equation. The theoretical analysis and experimental results show that the proposed algorithm needs no detection threshold, which has higher accuracy in the noise detection, is effectively able to remove the mixed noise, and can preserve the image details and the edges as well. The proposed algorithm has better subjective vision effect and objective quality than the wiener filter, median filter and other related algorithms. This algorithm put up better filter performance, high signal-to-noise ratio, denoising ability and strong adaptability.