Image to insight: exploring natural products through mass spectrometry imaging

Dong, Yonghui

doi:10.1039/d2np00011c

Cited by 37 publications

(27 citation statements)

References 133 publications

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“…In parallel with the development of MRI as a tool to noninvasively investigate plant structure and function, there has also been an increase in the use of MSI as a means of localizing specific metabolites (Sturtevant et al ., 2016; Qin et al ., 2018; Dong & Aharoni, 2022). The physical basis of MSI is the ionization of compounds, followed by their separation in a mass spectrometer.…”

Section: Mass Spectrometry Imaging – a Chemical Imaging Platformmentioning

confidence: 99%

Seeing plants as never before

et al. 2023

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Summary Imaging has long supported our ability to understand the inner life of plants, their development, and response to a dynamic environment. While optical microscopy remains the core tool for imaging, a suite of novel technologies is now beginning to make a significant contribution to visualize plant metabolism. The purpose of this review was to provide the scientific community with an overview of current imaging methods, which rely variously on either nuclear magnetic resonance (NMR), mass spectrometry (MS) or infrared (IR) spectroscopy, and to present some examples of their application in order to illustrate their utility. In addition to providing a description of the basic principles underlying these technologies, the review discusses their various advantages and limitations, reveals the current state of the art, and suggests their potential application to experimental practice. Finally, a view is presented as to how the technologies will likely develop, how these developments may encourage the formulation of novel experimental strategies, and how the enormous potential of these technologies can contribute to progress in plant science.

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Section: Mass Spectrometry Imaging – a Chemical Imaging Platformmentioning

confidence: 99%

Seeing plants as never before

et al. 2023

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“…Spatial metabolomics is a field of omics research focused on detecting and interpreting metabolites in the spatial context of cells, tissues, organs, and organisms 40 . Mass spectrometry imaging (MSI) is one of the most noteworthy techniques in spatial metabolomics studies, with the achievable spatial resolution down to cellular and subcellular levels to date 41,42 . While the molecular coverage is typically low and metabolite identification is challenging in MSI [43][44][45] .…”

Section: Discussionmentioning

confidence: 99%

How do Vampires Suck Blood?

Gou

Duan

et al. 2022

Preprint

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Lampreys are blood-sucking vampires in the marine. From a survival perspective, it is expected that lamprey buccal gland exhibits a repository of pharmacologically active components to modulate the homeostasis of host, inflammatory and immune responses. Several proteins have been found to function as anticoagulants, ion channel blockers, and immune suppressors in lampreys, while small metabolites have never been explored in detail. In this study, by analyzing the metabolic profiles of 14 different lamprey tissues, we have identified two groups of blood-sucking-associated metabolites, i.e., kynurenine pathway metabolites and prostaglandins, in the buccal gland and they can be injected into the host fish to ensure a steady and sustained blood flow to the feeding site. These findings demonstrate the complex nature of lamprey buccal gland and highlight the diversity in the mechanisms utilized for blood-sucking in lampreys. In addition, a lamprey spatial metabolomics database (https://www.lampreydb.com) was constructed to assist studies using lampreys as model animal. The database contains detailed qualitative, quantitative, and spatial distribution information of each detected metabolite, and users can easily query and check their metabolites of interest, and/or identify unknown peaks using the database.

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“…Coupling IMS to MSI as well as integrating and comparing spectral data from high resolution mass spectrometry (HRMS) of extracts from the same tissue are likely to improve metabolite assignment. Multimodal sampling of the same tissue as, for example, through multiplexed ion beam imaging (MiBI) or nano-desorbtion electrospray ionization (DESI) performing spatial protein mapping, transmission electron microscopy (TEM), fluorescent microscopy, and cytological stains could be assisting in elucidation of unknown structures ( 119 ) as could the adoption of orthogonal techniques such as photothermal infrared and Raman which can readily image single bacterial cells ( 120 , 121 ). Extending metabolomics studies to organisms under multiple conditions (i.e., various biotic and abiotic stresses) and in the course of interactions with other organisms, as well as probing more natural diversity and exotic species, could further increase the number of metabolites detected and comprehensiveness of metabolomics analysis.…”

Section: Perspectivementioning

confidence: 99%

Plant and microbial sciences as key drivers in the development of metabolomics research

Aharoni

Goodacre

Fernie

2023

Proc. Natl. Acad. Sci. U.S.A.

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This year marks the 25th anniversary of the coinage of the term metabolome [S. G. Oliver et al ., Trends Biotech. 16 , 373–378 (1998)]. As the field rapidly advances, it is important to take stock of the progress which has been made to best inform the disciplines future. While a medical-centric perspective on metabolomics has recently been published [M. Giera et al ., Cell Metab. 34 , 21–34 (2022)], this largely ignores the pioneering contributions made by the plant and microbial science communities. In this perspective, we provide a contemporary overview of all fields in which metabolomics is employed with particular emphasis on both methodological and application breakthroughs made in plant and microbial sciences that have shaped this evolving research discipline from the very early days of its establishment. This will not cover all types of metabolomics assays currently employed but will focus mainly on those utilizing mass spectrometry–based measurements since they are currently by far the most prominent. Having established the historical context of metabolomics, we will address the key challenges currently facing metabolomics and offer potential approaches by which these can be faced. Most salient among these is the fact that the vast majority of mass features are as yet not annotated with high confidence; what we may refer to as definitive identification. We discuss the potential of both standard compound libraries and artificial intelligence technologies to address this challenge and the use of natural variance–based approaches such as genome-wide association studies in attempt to assign specific functions to the myriad of structurally similar and complex specialized metabolites. We conclude by stating our contention that as these challenges are epic and that they will need far greater cooperative efforts from biologists, chemists, and computer scientists with an interest in all kingdoms of life than have been made to date. Ultimately, a better linkage of metabolome and genome data will likely also be needed particularly considering the Earth BioGenome Project.

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Image to insight: exploring natural products through mass spectrometry imaging

Abstract: This review provides an up-to-date summary of mass spectrometry imaging, emphasizing different applications and recent innovations in natural product research.

Cited by 37 publications

References 133 publications

Seeing plants as never before

Seeing plants as never before

How do Vampires Suck Blood?

Plant and microbial sciences as key drivers in the development of metabolomics research

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