Imaging analysis of early DMP1 mediated dentine remineralization

Bedran-Russo, Ana K.; Ravindran, Sriram; George, Anne

doi:10.1016/j.archoralbio.2012.09.007

Cited by 13 publications

(12 citation statements)

References 31 publications

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“…Phosphoric acid did not solubilize collagen or GAGs and resulted in minimal detection of PGs (Bedran-Russo et al 2013). Therefore, it is reasonable to suggest that our milder EDTA treatment should not have affected any of these organic components; however, these ECM molecules were not investigated (B) corresponding SAED diffraction pattern for mineral crystal nucleated by pB that displays a diffuse (2 1 1) ring that is characteristic of impure hydroxyapatite (HA); (C) representative mineral crystal nucleated by a 1:4 ratio of pA:pB; (D) SAED diffraction pattern corresponding to mineral nucleated by a 1:4 ratio of peptides, resulting in a (0 0 2) ring as well as several concentric rings, indicating the presence of rod-like HA crystals.…”

Section: Discussionmentioning

confidence: 99%

DMP1-derived Peptides Promote Remineralization of Human Dentin

et al. 2015

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Remineralization of dentin during dental caries is of considerable clinical interest. Dentin matrix protein 1 (DMP1) is a non-collagenous calcium-binding protein that plays a critical role in biomineralization. In the present study, we tested if peptides derived from DMP1 can be used for dentin remineralization. Peptide pA (pA, MW = 1.726 kDa) and peptide pB (pB, MW = 2.185), containing common collagen-binding domains and unique calcium-binding domains, were synthesized by solid-phase chemistry. An extreme caries lesion scenario was created by collagenase digestion, and the biomineral-nucleating potential of these peptides was ascertained when coated on collagenase-treated dentin matrix and control, native human dentin matrix under physiological levels of calcium and phosphate. Scanning electron microscopy analysis suggests that peptide pB was an effective nucleator when compared with pA. However, a 1:4 ratio of pA to pB was determined to be ideal for dentin remineralization, based on hydroxyapatite (HA) morphology and calcium/phosphorus ratios. Interestingly, HA was nucleated on collagenase-challenged dentin with as little as 20 min of 1:4 peptide incubation. Electron diffraction confirmed the presence of large HA crystals that produced a diffraction pattern indicative of a rod-like crystal structure. These findings suggest that DMP1-derived peptides may be useful to modulate mineral deposition and subsequent formation of HA when exposed to physiological concentrations of calcium and phosphate.

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Section: Discussionmentioning

confidence: 99%

DMP1-derived Peptides Promote Remineralization of Human Dentin

et al. 2015

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“…The demineralized dentin wafer tissues (kind gift from Dr. Bedran-Russo, Department of Restorative Density, UIC) were treated with trypsin to remove the noncollagenous proteins as published earlier3435. The wafers were dehydrated in a series of ethanol from 30 to 100%, trimmed, embedded in epoxy resin and ultrathin sections (70 nm) were placed on 300 mesh formvar carbon coated nickel grids.…”

Section: Methodsmentioning

confidence: 99%

TGF beta receptor II interacting protein-1, an intracellular protein has an extracellular role as a modulator of matrix mineralization

Ramachandran

Ravindran

Huang

et al. 2016

Sci Rep

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Transforming growth factor beta receptor II interacting protein 1 (TRIP-1), a predominantly intracellular protein is localized in the ECM of bone. TRIP-1 lacks a signal peptide, therefore, in this study, we provide evidence that intracellular TRIP-1 can be packaged and exported to the ECM via exosomes. Overexpression of TRIP-1 in MC3T3-E1 cells resulted in increased matrix mineralization during differentiation and knockdown resulted in reduced effects. In vivo function of TRIP-1 was studied by an implantation assay performed using TRIP-1 overexpressing and knockdown cells cultured in a 3-dimmensional scaffold. After 4 weeks, the subcutaneous tissues from TRIP-1 overexpressing cells showed higher calcium and phosphate deposits, arranged collagen fibrils and increased expression of Runx2 and alkaline phosphatase. Nucleation studies on demineralized and deproteinized dentin wafer is a powerful tool to determine the functional role of noncollagenous proteins in matrix mineralization. Using this system, we provide evidence that TRIP-1 binds to Type-I collagen and can promote mineralization. Surface plasmon resonance analysis demonstrated that TRIP-1 binds to collagen with KD = 48 μM. SEM and TEM analysis showed that TRIP-1 promoted the nucleation and growth of calcium phosphate mineral aggregates. Taken together, we provide mechanistic insights of this intracellular protein in matrix mineralization.

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“…On the other hand, our results are in line with other studies that also observed an effect of DMPs on demineralized dentin, supporting the conclusion that DMPs promote the deposition and nucleation of HA. For example, Bedran-Russo et al showed in their study that “DMP1-treated samples promoted deposition of amorphous calcium phosphate (ACP) precursors and needle-shaped hydroxyapatite crystals surrounding collagen fibrils” [22]. Liu et al came to the conclusion that “synthetic peptides derived from DMP1 bind type I collagen and promote nucleation of HA” [23].…”

Section: Discussionmentioning

confidence: 99%

Remineralization of Artificial Dentin Caries Using Dentin and Enamel Matrix Proteins

et al. 2019

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To assess the remineralizing potential of dentin matrix proteins and enamel matrix derivatives (DMPs and EMDs) after application on artificially induced dentin lesions, given the hypothesis that these materials increase the mineral uptake, binding, and mineralization. Forty-eight caries-free human premolars were used. Teeth were cut, polished, and embedded, leaving an open window on the root surface, of which one-third was covered with a flowable composite to preserve the healthy untreated dentin. Then, samples were demineralized in Buskes solution for 33 days. A micro-CT scan prior to treatment was performed. Next, the samples were randomly allocated into four groups: (A) An untreated negative control (CON), (B) application of porcine dentin matrix proteins (DMP), (C) treatment with enamel matrix derivatives (EMD, Emdogain, Straumann), and (D) amine fluoride application (AMF, Elmex fluid, GABA). All samples were placed in artificial saliva for 21 days. A second micro-CT scan was performed, after which the change in gray scaling within a defined region of interest (0.25 mm3) was analyzed. ANCOVA was applied to discover statistical differences between the different treatments. Both, treatment with AMF; (P = 0.011 versus CON) as well as with DMP (P = 0.043 versus CON) yielded a statistically significant difference compared to the control treatment. EMD treatment was not found to differ (P > 0.05). Mainly the top layer of the defects showed clear signs of remineralization, which was also evident in CON. This study was able to visually confirm the remineralization potential of demineralized dentin especially after DMP application, which, however, did not outperform AMF. Based on this, additional studies combining proteins and fluorides are now warranted and ongoing.

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Imaging analysis of early DMP1 mediated dentine remineralization

Cited by 13 publications

References 31 publications

DMP1-derived Peptides Promote Remineralization of Human Dentin

DMP1-derived Peptides Promote Remineralization of Human Dentin

TGF beta receptor II interacting protein-1, an intracellular protein has an extracellular role as a modulator of matrix mineralization

Remineralization of Artificial Dentin Caries Using Dentin and Enamel Matrix Proteins

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