Imaging and 3D Reconstruction of De Novo Peptide Capsids

Santis, Emiliana De; Ryadnov, Maxim G.

doi:10.1007/978-1-0716-0928-6_10

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Amongst the techniques available, transmission electron microscopy (TEM), provides high resolution information at the nanoscale, playing a key role in defining quality control criteria during process development and manufacturing (De Santis and Ryadnov, 2021). Concomitant information on size, polydispersity, morphology, and structural integrity, can be obtained by TEM at nanoscale resolution, thus making the technique an invaluable tool for the characterization of viral and VLP-based vaccines.…”

Section: Analytics For Quality Attribute Assessmentmentioning

confidence: 99%

Quality assessment of virus-like particle: A new transmission electron microscopy approach

Magalhães

Santis²,

Hussein-Gore³

et al. 2022

Front. Mol. Biosci.

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Transmission electron microscopy (TEM) is a gold standard analytical method for nanoparticle characterization and is playing a valuable role in virus-like particle (VLP) characterization extending to other biological entities such as viral vectors. A dedicated TEM facility is a challenge to both small and medium-sized enterprises (SMEs) and companies operating in low-and-middle income countries (LMICs) due to high start-up and running costs. A low-voltage TEM solution with assisted image acquisition and analysis such as the MiniTEM system, coupled with Vironova Imaging and Analysis Software (VIAS) could provide an affordable and practical alternative. The MiniTEM system has a small footprint and software that enables semi-automated data collection and image analysis workflows using built-in deep learning methods (convolutional neural networks) for automation in analysis, increasing speed of information processing and enabling scaling to larger datasets. In this perspective we outline the potential and challenges in the use of TEM as mainstream analytical tool in manufacturing settings. We highlight the rationale and preliminary findings from our proof-of-concept study aiming to develop a method to assess critical quality attributes (CQAs) of VLPs and facilitate adoption of TEM in manufacturing settings. In our study we explored all the steps, from sample preparation to data collection and analysis using synthetic VLPs as model systems. The applicability of the method in product development was verified at pilot-scale during the technology transfer of dengue VLPs development from a university setting to an LMIC- based vaccine manufacturing company, demonstrating the applicability of this analytical technique to VLP vaccine characterization.

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Section: Analytics For Quality Attribute Assessmentmentioning

confidence: 99%

Quality assessment of virus-like particle: A new transmission electron microscopy approach

Magalhães

Santis²,

Hussein-Gore³

et al. 2022

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

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Biofoundries are a nucleating hub for industrial translation

Farzaneh

Freemont

2021

Synthetic Biology

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Contemporary synthetic biology embraces the entire innovation pipeline; it’s a transformative technology platform impacting new applications and improving existing industrial products and processes. However, challenges still emerge at the interface of up-stream and down-stream processes, integral to the value chain. It is now clear that biofoundries have a key role to play in addressing this; they provide unique and accessible infrastructure to drive the standardization necessary to deliver systematic design and engineering of biological systems and workflows. As for other biofoundries, the success of the London Biofoundry has been in part due to its expertise in establishing channels for industrial translation through its extensive strategic collaborations. It has also become cemented as a key component of various consortia and partnerships that serve the broader bioeconomy and industrial strategies. Adopting a networked approach enables links to be made between infrastructure, researchers, industrialists and policy makers to de-risk the economic challenges of scale-up, as well as contribute to the growing bioeconomy.

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Imaging and 3D Reconstruction of De Novo Peptide Capsids

Cited by 2 publications

References 31 publications

Quality assessment of virus-like particle: A new transmission electron microscopy approach

Quality assessment of virus-like particle: A new transmission electron microscopy approach

Biofoundries are a nucleating hub for industrial translation

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