2022
DOI: 10.1021/acs.accounts.2c00510
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Imaging and Editing the Phospholipidome

Abstract: Conspectus Membranes are multifunctional supramolecular assemblies that encapsulate our cells and the organelles within them. Glycerophospholipids are the most abundant component of membranes. They make up the majority of the lipid bilayer and play both structural and functional roles. Each organelle has a different phospholipid composition critical for its function that results from dynamic interplay and regulation of numerous lipid-metabolizing enzymes and lipid transporters. Because lipid structures and loc… Show more

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Cited by 16 publications
(9 citation statements)
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“…This Account begins with what cell biology demands from lipid tools rather than starting from a chemical, biochemical, or biophysical origin. For lipid structures and classes as well as key techniques to analyze lipids, I refer to the expert articles in this special issue and elsewhere. Through extensive work over the past decades, we are quite well educated about the biosynthesis, metabolism, and typical location of lipids in cells. However, the cellular function of particular lipid species and their protein interactomes are largely elusive.…”
Section: Introductionmentioning
confidence: 99%
“…This Account begins with what cell biology demands from lipid tools rather than starting from a chemical, biochemical, or biophysical origin. For lipid structures and classes as well as key techniques to analyze lipids, I refer to the expert articles in this special issue and elsewhere. Through extensive work over the past decades, we are quite well educated about the biosynthesis, metabolism, and typical location of lipids in cells. However, the cellular function of particular lipid species and their protein interactomes are largely elusive.…”
Section: Introductionmentioning
confidence: 99%
“…Coined bioorthogonal reactions, these ligations often fullfill the criteria of click chemistry as they are high-yielding and exhibit fast kinetics, and mostly belong to the cycloaddition family. Bioorthogonal chemistry has been applied successfully to diverse biological models over the past 20 years and has greatly facilitated in vivo , ex vivo , and in situ studies of the main classes of biomolecules such as glycans, , lipids, nucleic acids, and proteins exploiting a wide range of bioanalytical and bioimaging readout techniques. , Although many notable examples have been described in mammalian living systems, , there are surprisingly few reports in plants, despite the fact that they represent 82% of all living matter on earth and make up the first economic resource in terms of nutrition, renewable materials and renewable energies. Lignin is one of the main constituents of plant cell walls, with cellulose.…”
Section: Introductionmentioning
confidence: 99%
“…Complicating matters further is the fact that individual lysosomes can be difficult to resolve, as their median size (∼400 nm) is close to the diffraction limit . Super-resolution microscopy (SRM) is a promising technique for imaging small vesicles, but it requires SRM-compatible dyes that emit in the far-red and are also bright and photostable. , While other subcellular organelles have been thoroughly investigated, , the low pH and small size of lysosomes make studying their dynamics a major challenge.…”
Section: Introductionmentioning
confidence: 99%