2015
DOI: 10.1038/nchembio.1959
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Imaging and manipulating proteins in live cells through covalent labeling

Abstract: The past 20 years have witnessed the advent of numerous technologies to specifically and covalently label proteins in cellulo and in vivo with synthetic probes. These technologies range from self-labeling proteins tags to non-natural amino acids, and the question is no longer how we can specifically label a given protein but rather with what additional functionality we wish to equip it. In addition, progress in fields such as super-resolution microscopy and genome editing have either provided additional motiva… Show more

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Cited by 210 publications
(202 citation statements)
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“…Importantly, the photophysical properties of 1 are retained in aqueous media (PBS buffer with 0.1% SDS). Similar to [8]CPP, the absorption maximum for 1 is at 328 nm with a large molar extinction coefficient of 5.8 x 10 4 M -1 cm -1 . Upon excitation, we observe a bright green fluorescence (lem = 510 nm) with a quantum yield of 0.17 and a large effective Stokes shift of over 180 nm.…”
Section: Introductionmentioning
confidence: 89%
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“…Importantly, the photophysical properties of 1 are retained in aqueous media (PBS buffer with 0.1% SDS). Similar to [8]CPP, the absorption maximum for 1 is at 328 nm with a large molar extinction coefficient of 5.8 x 10 4 M -1 cm -1 . Upon excitation, we observe a bright green fluorescence (lem = 510 nm) with a quantum yield of 0.17 and a large effective Stokes shift of over 180 nm.…”
Section: Introductionmentioning
confidence: 89%
“…Importantly, the nanohoop is completely soluble in DMSO with photophysical properties that are comparable to the parent nanohoop [8]CPP (Figure 2a). Of note, the installation of two sulfonates was sufficient to render this nanohoop aqueous-soluble, a result which is consistent with our findings that these nonplanar structures are much more soluble than flat aromatics.…”
Section: Introductionmentioning
confidence: 98%
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