Imaging and Quantitative Assessment of Long Bone Vascularization in the Adult Rat Using Microcomputed Tomography

Jia, Fu; Peyrin, Françoise; Malaval, Luc; Vico, Laurence; Lafage-Proust, Marie-Hélène

doi:10.1002/ar.21054

Cited by 39 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Micro-CT also provides three-dimensional (3D) images of bone architecture and various parameters that influence the strength of trabecular bone (e.g., trabecular bone volume, trabecular number, trabecular separation, trabecular thickness, and structure model index). However, because of the limited scan range, micro-CT is mostly used for the bones of small animals (rats or mice) or extracted human bones [12], [14], [15], and is applied more in laboratory-based research than in the clinical field of orthopedics. In clinical orthopedic practice, quantitative computed tomography (QCT) [5], [16], [17] or peripheral quantitative computed tomography (pQCT) [6], [9], [10], [18]–[20] are commonly used to assist in determining the bone BMD.…”

Section: Introductionmentioning

confidence: 99%

Predicting Cortical Bone Strength from DXA and Dental Cone-Beam CT

et al. 2012

View full text Add to dashboard Cite

ObjectiveThis study compared the capabilities of dual-energy X-ray absorptiometry (DXA) and dental cone-beam computed tomography (CBCT) for predicting the cortical bone strength of rat femurs and tibias.Materials and MethodsSpecimens of femurs and tibias obtained from 14 rats were first scanned with DXA to obtain the areal bone mineral density (BMD) of the midshaft cortical portion of the bones. The bones were then scanned using dental CBCT to measure the volumetric cortical bone mineral density (vCtBMD) and the cross-sectional moment of inertia (CSMI) for calculating the bone strength index (BSI). A three-point bending test was conducted to measure the fracture load of each femur and tibia. Bivariate linear Pearson analysis was used to calculate the correlation coefficients (r values) among the CBCT measurements, DXA measurements, and three-point bending parameters.ResultsThe correlation coefficients for the associations of the fracture load with areal BMD (measured using DXA), vCtBMD (measured using CBCT), CSMI (measured using CBCT), and BSI were 0.585 (p = 0.028) and 0.532 (p = 0.050) (for the femur and tibia, respectively), 0.638 (p = 0.014) and 0.762 (p = 0.002), 0.778 (p = 0.001) and 0.792 (p<0.001), and 0.822 (p<0.001) and 0.842 (p<0.001), respectively.ConclusionsCBCT was found to be superior to DXA for predicting cortical bone fracture loads in rat femurs and tibias. The BSI, which is a combined index of densitometric and geometric parameters, was especially useful. Further clinical studies are needed to validate the predictive value of BSI obtained from CBCT and should include testing on human cadaver specimens.

show abstract

Section: Introductionmentioning

confidence: 99%

Predicting Cortical Bone Strength from DXA and Dental Cone-Beam CT

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This is a disadvantage when studying bone and requires bone decalcification when using this contrast agent (Bentley et al ., ; Duvall et al ., ; Marxen et al ., ; Bolland et al ., ; Young et al ., ; Mondy et al ., , ; Nyangoga et al ., ; Roche et al ., ). We therefore chose barium sulphate for this study as recommended by a number of authors (Moore et al ., ; Duvall et al ., ; Marxen et al ., ; Oses et al ., ; Schneider et al ., ; Jia et al ., ; Roche et al ., ). We tested many mixtures, with different gelatin or barium sulphate percentages; the results were irrelevant or could not be reproduced.…”

Section: Discussionmentioning

confidence: 99%

“…We have made a lot of tests using these two contrast agents. Barium sulphate has been demonstrated to give better results as in the literature (Jia et al ., ; Prisby et al ., ; Roche et al ., , ) and was used in this study. Indeed, Microfil showed rounded artefacts, seems to change vascular anatomy and using it also need to decalcify the samples for study them by micro‐CT.…”

Section: Introductionmentioning

confidence: 98%

Vascular imaging with contrast agent in hard and soft tissues using microcomputed‐tomography

Bléry

Pilet

Vanden‐Bossche

et al. 2015

Journal of Microscopy

View full text Add to dashboard Cite

Vascularization is essential for many tissues and is a main requisite for various tissue-engineering strategies. Different techniques are used for highlighting vasculature, in vivo and ex vivo, in 2-D or 3-D including histological staining, immunohistochemistry, radiography, angiography, microscopy, computed tomography (CT) or micro-CT, both stand-alone and synchrotron system. Vascularization can be studied with or without a contrast agent. This paper presents the results obtained with the latest Skyscan micro-CT (Skyscan 1272, Bruker, Belgium) following barium sulphate injection replacing the bloodstream in comparison with results obtained with a Skyscan In Vivo 1076. Different hard and soft tissues were perfused with contrast agent and were harvested. Samples were analysed using both forms of micro-CT, and improved results were shown using this new micro-CT. This study highlights the vasculature using micro-CT methods. The results obtained with the Skyscan 1272 are clearly defined compared to results obtained with Skyscan 1076. In particular, this instrument highlights the high number of small vessels, which were not seen before at lower resolution. This new micro-CT opens broader possibilities in detection and characterization of the 3-D vascular tree to assess vascular tissue engineering strategies.

show abstract

“…For example, recent work has shown that prolonged skeletal disuse in hindlimb unloaded rats induces a narrowing of the maximal diameter of the femur PNA (39) and rarefaction of the microvascular network (14), both of which result in a lower femoral bone and marrow perfusion and vascular conductance with reloading (39). In contrast, 10–12 wks of treadmill exercise training increases the maximal diameter of the femur PNA and enhances the PNA endothelium-dependent vasodilation (12).…”

Section: Discussionmentioning

confidence: 99%

Exercise Training Augments Regional Bone and Marrow Blood Flow during Exercise

Stabley

Moningka

Behnke

et al. 2014

Medicine &Amp; Science in Sports &Amp; Exercise

View full text Add to dashboard Cite

Introduction The principal nutrient artery to the femur demonstrates an increase in nitric oxide mediated vasodilation in rats after treadmill exercise training. The present study sought to determine whether exercise training improves hindlimb bone and marrow blood flow distribution at rest and during exercise. Methods Six-eight month old male Sprague-Dawley rats were exercise trained (ET) with treadmill walking at 15 m/min up a 15° incline for 60 min/d over a 10–12 wk period. Sedentary (SED) control animals were acclimated to treadmill exercise for 5 min/d during the week preceding the blood flow measurements. Blood flow to nine distinct regions of the femur, tibia, and fibula were determined at rest and during low-intensity exercise (15 m/min walking, 0° incline) using the reference sample microsphere method. Results The results demonstrate an augmentation of exercise hyperemia above that observed in SED rats during exercise in only one region of bone, the femoral diaphysis of ET rats. However, while exercise hyperemia occurred in 3 of the 9 hindlimb bone regions measured in SED rats, exercise hyperemia occurred in 7 of 9 regions in ET rats. Conclusion These data indicate an increase in generalized hindlimb bone and marrow blood flow during physical activity following a period of exercise training. Elevations in regional bone and marrow blood flow after training may augment medullary pressure and bone interstitial fluid flow, thus benefiting bone integrity.

show abstract

Imaging and Quantitative Assessment of Long Bone Vascularization in the Adult Rat Using Microcomputed Tomography

Cited by 39 publications

References 28 publications

Predicting Cortical Bone Strength from DXA and Dental Cone-Beam CT

Predicting Cortical Bone Strength from DXA and Dental Cone-Beam CT

Vascular imaging with contrast agent in hard and soft tissues using microcomputed‐tomography

Exercise Training Augments Regional Bone and Marrow Blood Flow during Exercise

Contact Info

Product

Resources

About