Imaging and structural studies of DNA–protein complexes and membrane ion channels

Marini, Monica; Limongi, Tania; Falqui, Andrea; Genovese, Alessandro; Allione, Marco; Moretti, Manola; Lopatin, Sergei; Tirinato, Luca; Das, Gobind; Torre, Bruno; Giugni, Andrea; Cesca, Fabrizia; Benfenati, Fabio; Fabrizio, E. Di

doi:10.1039/c6nr07958j

Cited by 12 publications

(18 citation statements)

References 33 publications

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“…A 5 μl droplet of the DNA or of the DNA/intercalant solution was then deposited by a pipette over a super-hydrophobic device. The evaporation occurred on a hot plate at the constant temperature of 25 ˚C 53 , until the dewetting processes were completed. The samples were then imaged by SEM, working at an acceleration voltage of 5 kV and at 21 pA of current.…”

Section: Methodsmentioning

confidence: 99%

Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes

et al. 2019

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The effect of direct or indirect binding of intercalant molecules on DNA structure is of fundamental importance in understanding the biological functioning of DNA. Here we report on self-suspended DNA nanobundles as ultrasensitive nanomechanical resonators for structural studies of DNA-ligand complexes. Such vibrating nanostructures represent the smallest mechanical resonator entirely composed of DNA. A correlative analysis between the mechanical and structural properties is exploited to study the intrinsic changes of double strand DNA, when interacting with different intercalant molecules (YOYO-1 and GelRed) and a chemotherapeutic drug (Cisplatin), at different concentrations. Possible implications of our findings are related to the study of interaction mechanism of a wide category of molecules with DNA, and to further applications in medicine, such as optimal titration of chemotherapeutic drugs and environmental studies for the detection of heavy metals in human serum.

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Section: Methodsmentioning

confidence: 99%

Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes

et al. 2019

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“…On the contrary, Cr is much more resistant: few tens of nm of Cr can protect a substrate area from etching when drilling silicon up to few hundreds of micrometers in depth. Nevertheless, since the two metals can be deposited in sequence in the same process, it is easy to combine the final desired device property-pillars whose top is Au-covered-with the necessity to mask pillars from etching with a hard Cr mask [120].…”

Section: Shs For Manipulation Of Dna and Proteinsmentioning

confidence: 99%

Micro/Nanopatterned Superhydrophobic Surfaces Fabrication for Biomolecules and Biomaterials Manipulation and Analysis

et al. 2021

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Superhydrophobic surfaces display an extraordinary repulsion to water and water-based solutions. This effect emerges from the interplay of intrinsic hydrophobicity of the surface and its morphology. These surfaces have been established for a long time and have been studied for decades. The increasing interest in recent years has been focused towards applications in many different fields and, in particular, biomedical applications. In this paper, we review the progress achieved in the last years in the fabrication of regularly patterned superhydrophobic surfaces in many different materials and their exploitation for the manipulation and characterization of biomaterial, with particular emphasis on the issues affecting the yields of the fabrication processes and the quality of the manufactured devices.

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“…For this purpose, the Lambda DNA stock solution was resuspended in a buffer containing a 6.5 mM NaCl and 10 mM Tris-HCl, pH 9.3 and preheated as previously reported [6]. 5 l of the freshly prepared solution was deposited on the superhydrophobic device placed on a hot plate at 25˚ [16] until complete evaporation of the solution.…”

Section: Sample Preparationmentioning

confidence: 99%

“…During the dewetting process, the quasi-spherical shape of the drop is maintained while the volume is reduced, concentrating the molecules suspended in the solvent. At the end of the process the molecules accumulate [12,15] and/or suspend [6,16,17] The sample was then etched in a DRIE system to define the pillars, with a final height of the structures of about 10 m. Finally, the residual protecting layer of Cr on top of the pillars was removed with a selective wet etching.…”

Section: Introductionmentioning

confidence: 99%

Raman on suspended DNA: Novel super-hydrophobic approach for structural studies

Marini

Allione

Torre

et al. 2017

Microelectronic Engineering

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Imaging and structural studies of DNA–protein complexes and membrane ion channels

Cited by 12 publications

References 33 publications

Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes

Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes

Micro/Nanopatterned Superhydrophobic Surfaces Fabrication for Biomolecules and Biomaterials Manipulation and Analysis

Raman on suspended DNA: Novel super-hydrophobic approach for structural studies

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