Imaging-assisted hydrogel formation for single cell isolation

Oldenhof, Sander; Mytnyk, Serhii; Arranja, Alexandra; Puit, Marcel de; Esch, Jan H. van

doi:10.1038/s41598-020-62623-6

Cited by 8 publications

(7 citation statements)

References 43 publications

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“…The photoinitiator was synthesized according to the method described in the literature. , Under a nitrogen atmosphere, 2,4,6-trimethylbenzoyl chloride (4.8 g) was added dropwise to an equimolar amount of dimethyl phenylphosphonate (4.5 g). After stirring the reaction mixture at room temperature for 18 h, 150 mL of 2-butanone solution (containing 9.2 g of lithium bromide) was added.…”

Section: Methodsmentioning

confidence: 99%

Bioinspired Self-Resettable Hydrogel Actuators Powered by a Chemical Fuel

Bai

et al. 2022

ACS Appl. Mater. Interfaces

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The movements of soft living tissues, such as muscle, have sparked a strong interest in the design of hydrogel actuators; however, so far, typical manmade examples still lag behind their biological counterparts, which usually function under nonequilibrium conditions through the consumption of high-energy biomolecules and show highly autonomous behaviors. Here, we report on self-resettable hydrogel actuators that are powered by a chemical fuel and can spontaneously return to their original states over time once the fuels are depleted. Self-resettable actuation originates from a chemical fuel-mediated transient change in the hydrophilicity of the hydrogel networks. The actuation extent and duration can be programmed by the fuel levels, and the self-resettable actuation process is highly recyclable through refueling. Furthermore, various proof-of-concept autonomous soft robots are created, resembling the movements of soft-bodied creatures in nature. This work may serve as a starting point for the development of lifelike soft robots with autonomous behaviors.

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Section: Methodsmentioning

confidence: 99%

Bioinspired Self-Resettable Hydrogel Actuators Powered by a Chemical Fuel

Bai

et al. 2022

ACS Appl. Mater. Interfaces

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“…Qing Quan Liao et al [152] Emulsification (water-oil) Alginate MCF-7 Cell analysis Philipp S. Lienemann et al [52] Emulsification (water-oil) PEG MSC Cell analysis Dongguo Lin et al [153] Emulsification (water-oil) Alginate HCT116 and HT29 Cell analysis Audrey E. Mayfield et al [75] Manual drop-wise encapsulation Agarose CSC Cardiac therapy Ryo Negishi et al [74] Manual drop-wise encapsulation PEGDA HeLa, A549 and NCI-H1975 Cell analysis Simon Ng et al [154] Emulsification (water-oil) PEG-maleimide and DTT S. cerevisiae Enhancement of cell survival Sander Oldenhof et al [155] Imaging-assisted selective capture of cells…”

Section: Single-cell Encapsulation Enhancementmentioning

confidence: 99%

Unveiling the Potential of Single‐Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives

Pires‐Santos,

Nadine,

Mano

2024

Small Science

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The encapsulation of single cells has emerged as a promising field in recent years, owing to its potential applications in cell‐based therapeutics, bioprinting, in vitro cell culture, high‐throughput screening, and diagnostics. Single‐cell units offer several advantages, including compatibility with standard imaging techniques, superior diffusion rates, and lower material‐to‐cell volume ratios. They also serve as effective carriers for targeted drug delivery, allowing precise administration of therapeutics in cell‐mediated quantities. Moreover, single‐cell units exhibit improved circulation potential throughout the vasculature, with a reduced likelihood of entrapment compared to multicell strategies. However, the production of single‐cell units from random dispersion of cells follows the Poisson distribution, requiring the separation of empty and multicell units from single‐cell ones. Various methods have been developed to address this challenge; nevertheless, the majority of these strategies are either expensive or time‐consuming. This review provides an in‐depth analysis of the advantages and limitations of single‐cell units and their applications, as well as a comprehensive overview of the most used techniques for single‐cell encapsulation and sorting strategies.

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“…Then, cell attachment sites were visualized by fluorescent microscopy; and then through site-specific exposure to UV light, CD4 + and CD8 + lymphocytes were successfully released. Isolating individual cells allows novel microscopy techniques, such as laser capture microdissection, which cut off the block from the scaffold, and subsequently, appropriate enzymes are used to digest scaffold and release cells ( Oldenhof et al, 2020 ).…”

Section: Hematopoietic Stem Cells and Hematopoiesismentioning

confidence: 99%

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Zmrhal

Svoradová

Bátik

et al. 2022

Front. Cell Dev. Biol.

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Three-dimensional (3D) cell culture is attracting increasing attention today because it can mimic tissue environments and provide more realistic results than do conventional cell cultures. On the other hand, very little attention has been given to using 3D cell cultures in the field of avian cell biology. Although mimicking the bone marrow niche is a classic challenge of mammalian stem cell research, experiments have never been conducted in poultry on preparing in vitro the bone marrow niche. It is well known, however, that all diseases cause immunosuppression and target immune cells and their development. Hematopoietic stem cells (HSC) reside in the bone marrow and constitute a source for immune cells of lymphoid and myeloid origins. Disease prevention and control in poultry are facing new challenges, such as greater use of alternative breeding systems and expanding production of eggs and chicken meat in developing countries. Moreover, the COVID-19 pandemic will draw greater attention to the importance of disease management in poultry because poultry constitutes a rich source of zoonotic diseases. For these reasons, and because they will lead to a better understanding of disease pathogenesis, in vivo HSC niches for studying disease pathogenesis can be valuable tools for developing more effective disease prevention, diagnosis, and control. The main goal of this review is to summarize knowledge about avian hematopoietic cells, HSC niches, avian immunosuppressive diseases, and isolation of HSC, and the main part of the review is dedicated to using 3D cell cultures and their possible use for studying disease pathogenesis with practical examples. Therefore, this review can serve as a practical guide to support further preparation of 3D avian HSC niches to study the pathogenesis of avian diseases.

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Imaging-assisted hydrogel formation for single cell isolation

Cited by 8 publications

References 43 publications

Bioinspired Self-Resettable Hydrogel Actuators Powered by a Chemical Fuel

Bioinspired Self-Resettable Hydrogel Actuators Powered by a Chemical Fuel

Unveiling the Potential of Single‐Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

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