Imaging cilia in Drosophila melanogaster

Vieillard, Jennifer; Duteyrat, Jean-Luc; Cortier, Elisabeth; Durand, Bénédicte

doi:10.1016/bs.mcb.2014.12.009

Cited by 17 publications

(25 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two types of sense organ have such sensory neurons: external sense (ES) organs responsible for mechano-, chemo-, or olfactory-sensory functions, and chordotonal organs responsible for proprioception and hearing (Figure 5A) (Kernan 2007; Keil 2012; Vieillard et al 2015; Jana et al 2016). The cilia of type I neurons characteristically lack the central MT doublet.…”

Section: When the Centriole Becomes The Basal Body: Ciliogenesismentioning

confidence: 99%

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

Lattao¹,

Kovács²,

Glover

2017

Genetics

View full text Add to dashboard Cite

Centrioles play a key role in the development of the fly. They are needed for the correct formation of centrosomes, the organelles at the poles of the spindle that can persist as microtubule organizing centers (MTOCs) into interphase. The ability to nucleate cytoplasmic microtubules (MTs) is a property of the surrounding pericentriolar material (PCM). The centriole has a dual life, existing not only as the core of the centrosome but also as the basal body, the structure that templates the formation of cilia and flagellae. Thus the structure and functions of the centriole, the centrosome, and the basal body have an impact upon many aspects of development and physiology that can readily be modeled in Drosophila. Centrosomes are essential to give organization to the rapidly increasing numbers of nuclei in the syncytial embryo and for the spatially precise execution of cell division in numerous tissues, particularly during male meiosis. Although mitotic cell cycles can take place in the absence of centrosomes, this is an error-prone process that opens up the fly to developmental defects and the potential of tumor formation. Here, we review the structure and functions of the centriole, the centrosome, and the basal body in different tissues and cultured cells of Drosophila melanogaster, highlighting their contributions to different aspects of development and cell division.

show abstract

Section: When the Centriole Becomes The Basal Body: Ciliogenesismentioning

confidence: 99%

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

Lattao¹,

Kovács²,

Glover

2017

Genetics

View full text Add to dashboard Cite

show abstract

“…Centrosomes and cilia can be studied at all different stages of Drosophila development: in embryos, larvae, pupae, or adults [28][29][30]. In pupae and adults, the cuticles that encapsulate the sensory neurons are opaque and display autofluorescence making antibody staining and fluorescence live imaging difficult.…”

Section: Visualization Of Centrosomes and Cilia Using Fluorescence MImentioning

confidence: 99%

“…We also present protocols (see section 3.1 and 3.2) for immunostaining of sensory neurons ( Figure 3) and sperm cells ( Figure 4) in pupal/adult antenna and testes, respectively. Protocols to study these organelles in embryo and larvae, and for live imaging of the centrosome in testes can be found elsewhere [29,31,32].…”

Section: Visualization Of Centrosomes and Cilia Using Fluorescence MImentioning

confidence: 99%

“…Notably, longitudinal and cross sections of antennae can be used to study the cross and longitudinal section of the centrosomes/cilia, respectively ( Figure 5 A) [29]. Each chordotonal neuron grows a sensory cilium ( Figure 5B).…”

Section: Ultrastructure Of Centrosomes and Cilia In Drosophilamentioning

confidence: 99%

“…Studies in centriolar cells with or without cilia in the embryo are widely appreciated [28]. Moreover, dissection and imaging of the tissues that have ciliated cells in larvae, pupae, and adults are simple ( Figure 2) [20,29].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Methods to Study Centrosomes and Cilia in Drosophila

Jana

Mendonça

Werner

et al. 2016

Methods in Molecular Biology

View full text Add to dashboard Cite

Summary:Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile and intraflagellar transport (IFT)-independent or IFT-dependent assembly.Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.

show abstract

Evidence for intraflagellar transport in butterfly spermatocyte cilia

et al. 2023

View full text Add to dashboard Cite

In the model organism insect Drosophila melanogaster short cilia assemble on spermatocytes that elaborate into 1.8 mm long flagella during spermatid differentiation. A unique feature of these cilia/flagella is their lack of dependence on intraflagellar transport (IFT) for their assembly. Here, we show that in the common butterfly Pieris brassicae, the spermatocyte cilia are exceptionally long: about 40 μm compared to less than 1 μm in Drosophila. By transmission electron microscopy, we show that P. brassicae spermatocytes display several features not found in melanogaster, including compelling evidence of IFT structures and features of motile cilia.

show abstract

Imaging cilia in Drosophila melanogaster

Cited by 17 publications

References 55 publications

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

Methods to Study Centrosomes and Cilia in Drosophila

Evidence for intraflagellar transport in butterfly spermatocyte cilia

Contact Info

Product

Resources

About