Imaging fascicular organization of rat sciatic nerves with fast neural electrical impedance tomography

Ravagli, Enrico; Mastitskaya, Svetlana; Thompson, Nicole; Iacoviello, Francesco; Shearing, Paul R.; Perkins, Justin; Gourine, Alexander V.; Aristovich, Kirill; Holder, David

doi:10.1038/s41467-020-20127-x

Cited by 35 publications

(59 citation statements)

References 58 publications

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“…While spontaneous and evoked EIT recordings underwent different signal processing steps, all post-processed δV traces underwent the same image reconstruction process ( 22 , 24 ) (Fig. 3).…”

Section: Methodsmentioning

confidence: 99%

“…Fast neural Electrical Impedance Tomography (EIT) is a novel imaging method which enables tomographic imaging of localized compound action potential activity in fascicles within peripheral nerve. It employs a flexible silicone rubber cuff with 14 electrodes and averaging to a repeated trigger, such as the ECG, respiration, or electrically stimulated compound action potentials ( 8 ). The functional anatomy was determined with fast neural EIT as well as trial-and-error selective electrical stimulation using flexible nerve cuffs in vivo .…”

Section: Main Textmentioning

confidence: 99%

“…In peripheral nerves, FN-EIT is performed with a circumferential electrode array set on a nerve cuff, and thus is non-penetrating. It has been demonstrated in rat sciatic nerve with a resolution of 1 msec and <200µm (22)(23)(24)(25). The cuff contained 14 electrodes, each 1.50 × 0.35 mm, arranged radially around the long axis of the nerve.…”

Section: Eit Allows Imaging Of Organ-specific Activity Within the Vag...mentioning

confidence: 99%

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Organotopic Organization of the Cervical Vagus Nerve

Thompson

Ravagli

Mastitskaya

et al. 2022

Preprint

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The functional anatomy of fascicles evident in human and large mammal cervical vagus nerve is unknown. Organ-specific organization in the pig cervical nerve has been observed for cardiac, recurrent laryngeal and pulmonary function using anatomical tracing with X-ray microCT, and functional connectivity with selective stimulation or fast neural Electrical Impedance Tomography (EIT) with a custom nerve cuff. Electrical stimulation of the vagus nerve (VNS) is currently used to treat drug-resistant epilepsy and depression but current practice of stimulation of the entire nerve causes undesired side effects. These findings pave the way for improved outcomes in VNS as unwanted side effects could be reduced by targeted selective stimulation of identified organ-specific fascicles.

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Section: Methodsmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Eit Allows Imaging Of Organ-specific Activity Within the Vag...mentioning

confidence: 99%

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Organotopic Organization of the Cervical Vagus Nerve

Thompson

Ravagli

Mastitskaya

et al. 2022

Preprint

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“…Unfortunately, until recently, this was limited as the functional anatomy of fascicles in the vagus nerve was almost entirely unknown. In our group at University College London, we have developed a method to image localized fascicle compound action potential activity with Electrical Impedance Tomography (EIT) using a silicone rubber cuff with 14 circumferential electrodes (Figure 2A; Ravagli et al, 2019Ravagli et al, , 2020. Identified fascicles can then be selectively stimulated using two FIGURE 1 | Anti-inflammatory pathways of the vagus nerve.…”

Section: Vagus Nerve Stimulationmentioning

confidence: 99%

Selective Vagus Nerve Stimulation as a Therapeutic Approach for the Treatment of ARDS: A Rationale for Neuro-Immunomodulation in COVID-19 Disease

2021

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Acute respiratory distress syndrome (ARDS) is the most severe form of acute lung injury. It is induced by sepsis, aspiration, and pneumonia, including that caused by SARS coronavirus and human influenza viruses. The main pathophysiological mechanism of ARDS is a systemic inflammatory response. Vagus nerve stimulation (VNS) can limit cytokine production in the spleen and thereby dampen any systemic inflammation and inflammation-induced tissue damage in the lungs and other organs. However, the effects of increased parasympathetic outflow to the lungs when non-selective VNS is applied may result in bronchoconstriction, increased mucus secretion and enhance local pulmonary inflammatory activity; this may outweigh the beneficial systemic anti-inflammatory action of VNS. Organ/function-specific therapy can be achieved by imaging of localized fascicle activity within the vagus nerve and selective stimulation of identified organ-specific fascicles. This may be able to provide selective neuromodulation of different pathways within the vagus nerve and offer a novel means to improve outcome in ARDS. This has motivated this review in which we discuss the mechanisms of anti-inflammatory effects of VNS, progress in selective VNS techniques, and a possible application for ARDS.

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“…For instance, electric impedance of the giant axon of squids was studied in 1930 [ 12 , 13 ]. Recent developments in technologies for the measurement of impedance allow in vivo functional monitoring [ 14 ] and imaging of the fascicular organization of peripheral nerves [ 15 ]. Broadband dielectric spectroscopy (BDS) offers the broadest frequency range among all the methods for detection of the molecular dynamics in materials.…”

Section: Introductionmentioning

confidence: 99%

Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy

et al. 2021

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Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. Although nervous tissues have a membrane potential, the electrical reaction inside these tissues following electrical stimulation from outside remains unexplored. Furthermore, the pathophysiological reaction of an injured nerve is unclear. Here, we investigated the electrical reaction of injured and non-injured rat sciatic nerves via broadband dielectric spectroscopy. Crush injured and non-injured sciatic nerves of six 12-week-old male Lewis rats were used, 6 days after infliction of the injury. Both sides of the nerves (with and without injury) were exposed, and impedance measurements were performed at room temperature (approximately 25°C) at frequencies ranging from 100 mHz to 5.5 MHz and electric potential ranging from 0.100 to 1.00 V. The measured interfacial polarization potentially originated from the polarization by ion transport around nerve membranes at frequencies between 3.2 kHz and 1.6 MHz. The polarization strength of the injured nerves was smaller than that of non-injured nerves. However, the difference in polarization between injured and non-injured nerves might be caused by inflammation and edema. The suitable frequency range of the interfacial polarization can be expected to be critical for electrical stimulation of injured peripheral nerves.

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Imaging fascicular organization of rat sciatic nerves with fast neural electrical impedance tomography

Cited by 35 publications

References 58 publications

Organotopic Organization of the Cervical Vagus Nerve

Organotopic Organization of the Cervical Vagus Nerve

Selective Vagus Nerve Stimulation as a Therapeutic Approach for the Treatment of ARDS: A Rationale for Neuro-Immunomodulation in COVID-19 Disease

Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy

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