Imaging flow cytometry and fluorescence microscopy in assessing radiation response in lymphocytes from umbilical cord blood and cancer patients

Durdik, Matus; Košík, Pavol; Jakl, Lukas; Kozackova, Maria; Markovà, Eva; Vigasova, Katarina; Beresova, Katarina; Jakubíková, Jana; Horváthová, Eva; Zastko, Lucián; Fekete, Marta; Závacká, Ingrid; Pobijakova, M.; Belyaev, Igor

doi:10.1002/cyto.a.24468

Cited by 9 publications

(2 citation statements)

References 31 publications

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“…The fluorescein isothiocyanate (FITC)-Annexin V apoptosis detection kit (Abcam Inc., Cambridge Science Park, Cambridge, UK) was used in conjunction with two fluorescent channels flowcytometry to evaluate apoptosis and necrosis cell populations (Henidi et al 2020;Durdik et al 2021). After incubation of H460 and A549 cells separately with the tested compounds at 2xIC50 and doxorubicin 10 µM (positive control) for 48 hours, Trypsinization was used to extract cells, which were then washed twice with ice-cold PBS (pH 7.4).…”

Section: Detection Of Apoptosis Using Flow Cytometry Analysismentioning

confidence: 99%

Eurycomanol and eurycomanone as potent inducers for cell-cycle arrest and apoptosis in small and large human lung cancer cell lines

Okba

Ezzat

Shehabeldine

et al. 2022

Natural Product Research

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Eurycoma longifolia Jack is one of traditional herbal medicines in South-East Asia. This study evaluated the anticancer, cell-cycle arrest, and apoptotic induction potentials of eurycomanone (EONE) and eurycomanol (EOL), highly oxygenated quassinoids previously isolated from its roots, against large (H460) and small (A549) lung cancer cells. EOL and EONE exhibited IC50 of 386 and 424 µg/mL on normal human lung cell line. EONE exhibited higher anticancer activity with an IC50 of 1.78 µg/mL and 20.66 μg/mL than EOL which exhibited an IC50 of 3.22 µg/mL and 38.05 µg/mL against H460 and A549 respectively. Both reduced the viability of H460 and A549 and arrested G0/G1 phase. The increase in the apoptotic rates was mainly in the percentage of late apoptosis. Moreover, they inhibited A549 by inducing the accumulation of S and G2/M phases. This study revealed EOL and EONE potential as novel leads exhibiting cell-cycle arrest and apoptosis induction potentials.

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Section: Detection Of Apoptosis Using Flow Cytometry Analysismentioning

confidence: 99%

Eurycomanol and eurycomanone as potent inducers for cell-cycle arrest and apoptosis in small and large human lung cancer cell lines

Okba

Ezzat

Shehabeldine

et al. 2022

Natural Product Research

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“…Fluorescence Imaging Flow Cytometry (FIFC) supply, for each flowing cells in the field of view, a bright-field image, a dark-field image, and several fluorescence images [6]. FIFC has been recently exploited for assessing radiation response in lymphocytes (LCs) from umbilical cord blood and cancer patients [7]. Here we present a strategy based on the combination of microfluidics and stain-free imaging to monitor the morphological variation induced in cell population without the use of fluorescent tags.…”

Section: Introductionmentioning

confidence: 99%

Label-free imaging and microfluidics to diagnose space-induced effects on blood components

Miccio,

Behal,

Pirone

et al. 2023

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI

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Human health and disease prevention are among the priorities to safeguard astronauts and, in the next future, space tourists. There is a great demand of new reliable bio-technologies that would be eventually implemented on spacecrafts to observe the space-induced effect on humans. One of the main risks is related to the radiation exposure, that is significantly higher than on Earth. For this reason, space agencies are pushing to develop strategies to quantify, oversee and limiting such risks. Here we present an approach based on the combination of microfluidics and stain-free imaging also aided by artificial intelligence to monitor the effect on ionizing radiation on blood cells. The system is based on the Holographic Image Flow Cytometry system where Quantitative Phase Contrast images are retrieved for cell flowing and rotating into a microfluidics circuits. Proof of concept is demonstrated where morphological parameters are identified able to distinguish cell population irradiated at different radiation doses and at different time from the radiation exposure. Blood cell will be analyzed. The presented approach has main advantages respect to standard and already existent technologies for single cell analysis. The first one is the no-need of fluorescence staining thus opening to faster and easier operation steps. The second one is related to cell rotation into the field of view, allowing to acquire images at different rotation angle and thus collecting a broader dataset useful for the application of artificial intelligence network. Furthermore the system can be miniaturized to a scale portable out of the laboratory environment.

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Exposure to the plasticizer diisopentyl phthalate can cause Vero cell line death

Moscardi,

Irioda,

Mogharbel

et al. 2024

Food and Chemical Toxicology

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Imaging flow cytometry and fluorescence microscopy in assessing radiation response in lymphocytes from umbilical cord blood and cancer patients

Cited by 9 publications

References 31 publications

Eurycomanol and eurycomanone as potent inducers for cell-cycle arrest and apoptosis in small and large human lung cancer cell lines

Eurycomanol and eurycomanone as potent inducers for cell-cycle arrest and apoptosis in small and large human lung cancer cell lines

Label-free imaging and microfluidics to diagnose space-induced effects on blood components

Exposure to the plasticizer diisopentyl phthalate can cause Vero cell line death

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