Imaging flow cytometry reveals a dual role for exopolysaccharides in biofilms: To promote self-adhesion while repelling non-self-community members

Maan, Harsh; Povolotsky, Tatyana L.; Porat, Ziv; Malitsky, Sergey; Kolodkin‐Gal, Ilana

doi:10.1016/j.csbj.2021.11.043

Cited by 10 publications

(6 citation statements)

References 73 publications

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“…Next, we explored whether a difference in bacillaene production underlies the reduced inhibition by B. subtilis in nutrient-depleted conditions. Here, we measured the expression of the pks operon via a P pksC - yfp reporter fusion in nutrient-rich and nutrient-depleted conditions, since it has been previously established that activity of the P pksC promoter correlates well with bacillaene synthesis ( 45 , 46 ). In addition, because it has been shown that bacteria can alter the production of antimicrobials in the presence of competitors ( 45 , 47 , 48 ), we compared the expression between monoculture and coculture conditions ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It was previously reported that biofilm formation could alter the production of bacillaene and mediate the outcome of interspecies interactions ( 46 ). The extracellular matrix of the B. subtilis biofilm is composed of polysaccharides, proteins, and nucleic acids ( 27 , 40 , 51 ).…”

Section: Resultsmentioning

confidence: 99%

“…This diffusible polyketide antibiotic ( 12 , 17 , 63 ) inhibits the growth of various bacteria when purified or present in spent media ( 13 ). However, its influence on competition in a mixed-species biofilm has been less studied ( 17 , 19 – 21 , 46 ) and has not yet been addressed for B. subtilis - Salmonella interactions.…”

Section: Discussionmentioning

confidence: 99%

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Nutrient Availability and Biofilm Polysaccharide Shape the Bacillaene-Dependent Antagonism of Bacillus subtilis against Salmonella Typhimurium

et al. 2022

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Probiotic bacteria represent an alternative for controlling foodborne disease caused by Salmonella enterica , which constitutes a serious concern during food production due to its antibiotic resistance and resilience to environmental stress. Bacillus subtilis is gaining popularity as a probiotic, but its behavior in biofilms with pathogens such as Salmonella remains to be elucidated. Here, we show that the antagonism of B. subtilis is mediated by the polyketide bacillaene and that the production of bacillaene is a highly dynamic trait which depends on environmental factors such as nutrient availability and the presence of competitors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Nutrient Availability and Biofilm Polysaccharide Shape the Bacillaene-Dependent Antagonism of Bacillus subtilis against Salmonella Typhimurium

et al. 2022

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“…The supernatant of Lactiplantibacillus plantarum affects glucose metabolism and inhibits the expression of the virulence gene of C. albicans, which encodes an agglutinin-like protein [37]. Maan et al [38] discovered that EPS provides dual adaptive advantages for cells to form biofilms, which not only promote the co-aggregation of related species, but also inhibit the growth of incompatible species.…”

Section: Formation and Maturation Of Microcoloniesmentioning

confidence: 99%

Elimination of Pathogen Biofilms via Postbiotics from Lactic Acid Bacteria: A Promising Method in Food and Biomedicine

Che,

Shi,

Fang

et al. 2024

Microorganisms

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Pathogenic biofilms provide a naturally favorable barrier for microbial growth and are closely related to the virulence of pathogens. Postbiotics from lactic acid bacteria (LAB) are secondary metabolites and cellular components obtained by inactivation of fermentation broth; they have a certain inhibitory effect on all stages of pathogen biofilms. Postbiotics from LAB have drawn attention because of their high stability, safety dose parameters, and long storage period, which give them a broad application prospect in the fields of food and medicine. The mechanisms of eliminating pathogen biofilms via postbiotics from LAB mainly affect the surface adhesion, self-aggregation, virulence, and QS of pathogens influencing interspecific and intraspecific communication. However, there are some factors (preparation process and lack of target) which can limit the antibiofilm impact of postbiotics. Therefore, by using a delivery carrier and optimizing process parameters, the effect of interfering factors can be eliminated. This review summarizes the concept and characteristics of postbiotics from LAB, focusing on their preparation technology and antibiofilm effect, and the applications and limitations of postbiotics in food processing and clinical treatment are also discussed.

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“…Colony forming units (CFUs) were then counted after the 20-hour growth curve using the resultant culture as described in Maan et al 50 Briefly, the culture was transferred to a 96-well plate and a serial dilution from 10 0 to 10 -7 was performed in DPBS. 20 µL of varying dilutions were spotted onto LB agar plates and allowed to dry completely in clean bench hood.…”

Section: Analysis Of the Swelling Capacity Of Hydrogelsmentioning

confidence: 99%

Lignin-based Mucin-mimicking Antiviral Hydrogels with Enzyme Stability and Tunable Porosity

Chandna,

L. Povolotsky,

Nie

et al. 2024

Preprint

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Mucus is a complex hydrogel acting as a defensive and protective barrier in various parts of the human body. The structure and composition of mucus play an important role in maintaining barrier properties by acting as a filter for the diffusion of biomolecules and pathogens. The rise in viral infections has underscored the importance of advancing research into mucus-mimicking hydrogels for the efficient design of antiviral agents. However, the performance of an antiviral strategy should not only be assessed based on its efficacy in inhibiting infections but also based on its sustainability. Herein, we demonstrate the gram-scale synthesis of biocompatible, lignin-based virus-binding inhibitors that reduce waste and ensure long-term availability. The lignin-based inhibitors were equipped with sulfate moieties, which are known binding partners for many viruses including SARS-CoV-2 and herpes viruses. In addition, crosslinking the synthesized inhibitors yielded hydrogels that mimicked native mucus with respect to surface functionality and rheology. It is found that the degree of sulfation has a very strong impact on the mesh size distribution of the hydrogels, which provides a new means to fine-tune steric and electrostatic contributions of the virus-hydrogel interaction. This feature strongly impacts the sequestration capability of the lignin-based hydrogels, which is demonstrated by infection inhibition assays involving human herpes simplex virus-1, influenza A viruses, and the bacterium Escherichia coli (E. coli). For HSV-1 and E. coli, these measurements showed a reduction in plaque (HSV-1) and colony-forming units (E. coli) by more than 4 orders of magnitude, indicating potent inhibition by the lignin-based hydrogels. Taken together, the sulfated lignin hydrogel is an excellent scaffold for large-scale synthesis of sustainable, biocompatible, and highly efficient pathogen-binding inhibitors.

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Imaging flow cytometry reveals a dual role for exopolysaccharides in biofilms: To promote self-adhesion while repelling non-self-community members

Abstract: Graphical abstract

Cited by 10 publications

References 73 publications

Nutrient Availability and Biofilm Polysaccharide Shape the Bacillaene-Dependent Antagonism of Bacillus subtilis against Salmonella Typhimurium

Nutrient Availability and Biofilm Polysaccharide Shape the Bacillaene-Dependent Antagonism of Bacillus subtilis against Salmonella Typhimurium

Elimination of Pathogen Biofilms via Postbiotics from Lactic Acid Bacteria: A Promising Method in Food and Biomedicine

Lignin-based Mucin-mimicking Antiviral Hydrogels with Enzyme Stability and Tunable Porosity

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