Imaging: Gear up for mechano-immunology

Wan, Zhengpeng; Shaheen, Samina; Chau, Alicia; Zeng, Yingyue; Liu, Wanli

doi:10.1016/j.cellimm.2019.103926

Cited by 5 publications

(3 citation statements)

References 138 publications

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“…Shear stress which is an inherently present force in dynamic systems and it is well known that physical forces • Prolonged maintenance of telomer length [47] affect MSC properties, such as their differentiation capacity [55]. Furthermore, it is known that immune cells, such as B and T lymphocytes, have mechanosensing capabilities and that shear forces affect their immunomodulatory properties [56]. However, only a few studies investigated the impact of shear forces on the immunomodulatory and anti-inflammatory properties of MSCs.…”

Section: Immunomodulatory and Anti-inflammatory Effectsmentioning

confidence: 99%

Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell‐based therapy products

Egger

Lavrentieva

Kugelmeier³

et al. 2021

Engineering in Life Sciences

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The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It is essential to consider all steps along the in vitro fabrication of cell-based products in order to reach efficient and reproducible treatment outcomes. Here, the optimal protocols for isolation, cultivation and differentiation of mesenchymal stem cells are required. In this review we discuss these aspects and their influence on the final cell-based product quality. We demonstrate that physiological in vitro cell cultivation conditions play a crucial role in therapeutic functionalities of cultivated cells. We show that three-dimensional cell culture, dynamic culture conditions and physiologically relevant in vitro oxygen concentrations during isolation and expansion make a decisive contribution towards the improvement of cell-based products in regenerative medicine.

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Section: Immunomodulatory and Anti-inflammatory Effectsmentioning

confidence: 99%

Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell‐based therapy products

Egger

Lavrentieva

Kugelmeier³

et al. 2021

Engineering in Life Sciences

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“…Several studies have reported that B lymphocytes (B cells) producing effective SARS-CoV-2 specific NAbs (IgG, IgM, IgE) have been successfully isolated and identified in convalescent patient's plasma [25]. Multiple technical platforms, including immunology, high-speed, high-resolution livecell single-molecule fluorescence imaging technologies, have illustrated potential mechanisms to uncover a series of events showing how B cells undergo Ab production [26]. Here we review the literature, highlighting how nanoscale signaling immune (B and T) cells contribute to COVID-19 mediated infection.…”

Section: Severe Acute Respiratory Syndrome Coronavirus 2 Targeted Antibodies Cocktail and B Cell Receptor Interplay: Interventions To Trimentioning

confidence: 99%

“…These events lead to several well-ordered molecular events, including isotype class switching, affinity maturation, and Ab production against SARS-CoV-2 [5,20,21]. BCR's intricate, methodical configuration reveals efficient capability to translate the external physical, chemical, and antigenic cues and harbor sophisticated mechanisms of generating chemical signal entities to reshape the immune mechanism [22,26]. However, potential investigations on how BCRs of SARS-CoV-2 specific B cells isolated from CP of infected people respond to BCR signaling, spatiotemporal dynamics, and Ab production remains obscure.…”

Section: B Cell Receptor Signaling and The Generation Of Protective Abs During Sars-cov-2 Infectionmentioning

confidence: 99%

Severe acute respiratory syndrome coronavirus 2 targeted antibodies cocktail and B cell receptor interplay: interventions to trigger vaccine development

Haneef

Saleem²,

Khan

et al. 2021

Explor Immunol

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Coronavirus disease-2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS-CoV)-2 spread globally and creates an alarming situation. Following the SARS-CoV-2 paradigm, therapeutic efficacy is achieved via repurposing several antiviral, antibacterial, and antimalarial drugs. Innate and adaptive immune cells work close to combat infection through the intricate production of antibodies (Abs) and inflammatory cytokines. As an essential component of the immune system, Abs play an important role in eliminating viruses and maintaining homeostasis. B lymphocytes (B cells) are effector cells, stringent to produce neutralizing Abs to combat infection. After recognizing SARS-CoV-2 antigens by a surface receptor called B cell receptors (BCRs) on the plasma membrane, the BCRs transmembrane signal transduction and immune activation results in Ab production and development of immune memory. Thus, it ensures that plasma B cells can quickly start an intricate immune response to generate efficient protective Abs to clear the pathogen. Nevertheless, considering therapeutic challenges in the context of the new coronavirus pandemic, this review addresses the molecular mechanism of the immune activation and function of novel SARS-CoV-2 specific B cells in the production of SARS-CoV-2 specific Abs. Additionally, these studies highlighted the Ab-mediated pathogenesis, the intriguing role of nano-scale signaling subunits, non-structural proteins during COVID-19 infection, and structural insights of SARS-CoV-2 specific Abs.

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