Imaging growth of neurites in conditioned hydrogel by coherent anti-Stokes Raman scattering microscopy

Conovaloff, Aaron W.; Wang, Hanwei; Cheng, Ji‐Xin; Panitch, Alyssa

doi:10.4161/org.5.4.10404

Cited by 14 publications

(16 citation statements)

References 46 publications

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“…When the beat frequency (v p 2 v S ) matches the frequency of a particular Raman-active molecular vibration (v vib ), the resonant oscillators are coherently driven, resulting in a strong antiStokes signal at v as ¼ 2v p 2 v S [158]. The technique was applied to study osteogenic and adipogenic differentiation [73], scaffolds and hydrogels [12,76,159,160], vascular and neurodegenerative diseases [38,161] and drug delivery and subcellular distribution (reviewed in Strachan et al [162]). CARS microscopy has proved useful for mapping lipid compartments in various tissues and cells [158,163], protein clusters [164] and water distributions [161] in cell tissue cultures and has been used with great success for imaging of many types of lipids (C -H vibration) and phosphates [165].…”

Section: Three-photon-based Nonlinear Optical Microscopy Techniquesmentioning

confidence: 99%

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

Vielreicher

Schürmann

Detsch

et al. 2013

J. R. Soc. Interface.

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This review focuses on modern nonlinear optical microscopy (NLOM) methods that are increasingly being used in the field of tissue engineering (TE) to image tissue non-invasively and without labelling in depths unreached by conventional microscopy techniques. With NLOM techniques, biomaterial matrices, cultured cells and their produced extracellular matrix may be visualized with high resolution. After introducing classical imaging methodologies such as µCT, MRI, optical coherence tomography, electron microscopy and conventional microscopy two-photon fluorescence (2-PF) and second harmonic generation (SHG) imaging are described in detail (principle, power, limitations) together with their most widely used TE applications. Besides our own cell encapsulation, cell printing and collagen scaffolding systems and their NLOM imaging the most current research articles will be reviewed. These cover imaging of autofluorescence and fluorescence-labelled tissue and biomaterial structures, SHG-based quantitative morphometry of collagen I and other proteins, imaging of vascularization and online monitoring techniques in TE. Finally, some insight is given into state-of-the-art three-photon-based imaging methods (e.g. coherent anti-Stokes Raman scattering, third harmonic generation). This review provides an overview of the powerful and constantly evolving field of multiphoton microscopy, which is a powerful and indispensable tool for the development of artificial tissues in regenerative medicine and which is likely to gain importance also as a means for general diagnostic medical imaging.

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Section: Three-photon-based Nonlinear Optical Microscopy Techniquesmentioning

confidence: 99%

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

Vielreicher

Schürmann

Detsch

et al. 2013

J. R. Soc. Interface.

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show abstract

“…[5][6][7][8][9][10][11] The primary advantages of CARS microscopy over other label-free techniques are its rapid imaging capabilities, which extend up to video rate, 12 and intrinsic confocality, allowing three-dimensional sectioning without descanning beams or the installation of pinholes. We recently introduced a technique for hyperspectral imaging that combines the imaging speed of CARS with the wide tunability of an optical parametric oscillator (OPO).…”

Section: Introductionmentioning

confidence: 99%

In plantaimaging of Δ9-tetrahydrocannabinolic acid inCannabis sativa L.with hyperspectral coherent anti-Stokes Raman scattering microscopy

et al. 2013

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Abstract. Nature has developed many pathways to produce medicinal products of extraordinary potency and specificity with significantly higher efficiencies than current synthetic methods can achieve. Identification of these mechanisms and their precise locations within plants could substantially increase the yield of a number of natural pharmaceutics. We report label-free imaging of Δ 9 -tetrahydrocannabinolic acid (THCa) in Cannabis sativa L. using coherent anti-Stokes Raman scattering microscopy. In line with previous observations we find high concentrations of THCa in pistillate flowering bodies and relatively low amounts within flowering bracts. Surprisingly, we find differences in the local morphologies of the THCa-containing bodies: organelles within bracts are large, diffuse, and spheroidal, whereas in pistillate flowers they are generally compact, dense, and have heterogeneous structures. We have also identified two distinct vibrational signatures associated with THCa, both in pure crystalline form and within Cannabis plants; at present the exact natures of these spectra remain an open question. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…It can be combined with other nonlinear techniques (2PM, SHG) and provide additional chemical information. So far, CARS has been used to image lipids and exogenous mineral oils 49,225 in cell cultures, 31,150,153,167,209 ex vivo and in vivo in small animals 49,50,56,119,125,153,225,226 or ex vivo human samples. 60 Recently Evans et al 49 reported a CARS microscope capable of real time video rate imaging (20 fps) in small animals in vivo.…”

Section: Nonlinear Microscopy (2-photon Microscopy Second Harmonic Gmentioning

confidence: 99%

Optical and Opto-Acoustic Interventional Imaging

2012

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Many clinical interventional procedures, such as surgery or endoscopy, are today still guided by human vision and perception. Human vision however is not sensitive or accurate in detecting a large range of disease biomarkers, for example cellular or molecular processes characteristic of disease. For this reason advanced optical and opto-acoustic (photo-acoustic) methods are considered for enabling a more versatile, sensitive and accurate detection of disease biomarkers and complement human vision in clinical decision making during interventions. Herein, we outline developments in emerging fluorescence and opto-acoustic sensing and imaging techniques that can lead to practical implementations toward improving interventional vision.

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Imaging growth of neurites in conditioned hydrogel by coherent anti-Stokes Raman scattering microscopy

Cited by 14 publications

References 46 publications

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

In plantaimaging of Δ9-tetrahydrocannabinolic acid inCannabis sativa L.with hyperspectral coherent anti-Stokes Raman scattering microscopy

Optical and Opto-Acoustic Interventional Imaging

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