Imaging HOCl Generation during the Mitochondria Peripheral Fission with a Tailor-Made Fluorescent Probe

Activatable Fluorescent Probe for Studying Drug-Induced Senescence In Vitro and In Vivo

“…Consequently, aging stands as the primary risk factor linked to compromised tissue functionality and the onset of chronic ailments in humans. − Through extensive research on senescent cells, certain proteins and molecules, including senescence-associated β-galactosidase (SA-β-gal), p16INK4a, p53, p21, and IGFBP5, have emerged as commonly employed biomarkers associated with cellular senescence. − Throughout the process of cellular senescence, the concentration of reactive oxygen species (ROS) typically undergoes a progressive rise and can be influenced by a multitude of factors, resulting in varying degrees of fluctuation. − Consequently, preserving the equilibrium of ROS assumes significance in retarding cellular senescence. − HClO is a significant ROS with oxidative properties in biological systems. It originates from the breakdown of neutral HOCl and acts as a potent oxidizing agent that interacts with biomolecules within cells. , HClO plays a role in regulating intracellular signaling, inflammatory responses, and immune processes. − However, excessive levels of HClO can induce cellular oxidative stress, leading to damage in cellular structure and function, which accelerates cellular senescence and disease progression. − Therefore, comprehensive examinations of HClO during senescence are vital for enhancing our understanding of cellular senescence mechanisms, preventing age-related diseases, and devising antiaging strategies.…”