Imaging methods for screening of hepatic steatosis

Hussain, Hero K.

doi:10.1002/9781118924938.ch16

Cited by 2 publications

(4 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NAFLD can cause wide sets of diseases associated with damaged liver, including steatosis, fibrosis, cirrhosis and can cause whole organ failure and morbidity [1][2][3]. NAFLD is usually imaged with Ultrasound, CT or MRI [4][5][6]. To estimate the extent of the damage and effectiveness of the treatment, often biopsies are carried out and stained and imaged by trained pathologists.…”

Section: Introductionmentioning

confidence: 99%

“…To estimate the extent of the damage and effectiveness of the treatment, often biopsies are carried out and stained and imaged by trained pathologists. Traditional histological techniques for liver biopsy imaging and analysis involve tissue staining with dyes specific for specific constituents and then optical imaging [5,6]. A second imaging technique often employed is immunofluorescence microscopy [7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy

Ranjit

Dvornikov

Dobrinskikh

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

The phasor approach to auto-fluorescence lifetime imaging was used to identify and characterize a long lifetime species (LLS) (~7.8 ns) in livers of mice fed with a Western diet. The size of the areas containing this LLS species depends on the type of diet and the size distribution shows Western diet has much larger LLS sizes. Combination of third harmonic generation images with FLIM identified the LLS species with fat droplets and the droplet size distribution was estimated. Second harmonic generation microscopy combined with phasor FLIM shows that there is an increase in fibrosis with a Western diet. A new decomposition in three components of the phasor plot shows that a Western diet is correlated with a higher fraction of free NADH, signifying more reducing condition and more glycolytic condition. Multiparametric analysis of phasor distribution shows that from the distribution of phasor points, a Western diet fed versus a low fat diet fed samples of mice livers can be separated. The phasor approach for the analysis of FLIM images of autofluorescence in liver specimens can result in discovery of new fluorescent species and then these new fluorescent species can help assess tissue architecture. Finally integrating FLIM and second and third harmonic analysis provides a measure of the advancement of fibrosis as an effect of diet.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy

Ranjit

Dvornikov

Dobrinskikh

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…While liver biopsy is the gold standard for liver diagnostics, it is invasive and impractical for screening large populations [8,9]. Magnetic resonance imaging (MRI)-based methods are expensive and not widely available; computed tomography and ultrasound have intrinsic subjective and technical limitations [9,10]; and the fatty liver index (FLI) [11], hepatic steatosis index (HSI) [12], and NAFLD liver fat score (NAFLD-LFS) [13] lack sufficient validation or cannot quantify steatosis accurately [10,13,14]. Waist circumference (WC) measurement has been found to be as reliable as FLI [15,16], and waist circumference/height ratio (WCHR) is reportedly more reliable than WC, although the latter conclusion is questionable [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Waist circumference (WC) measurement has been found to be as reliable as FLI [15,16], and waist circumference/height ratio (WCHR) is reportedly more reliable than WC, although the latter conclusion is questionable [17,18]. So far, ultrasound remains the most accessible and frequently used screening diagnostic test [9], but insurance limitations in Russia reduce its availability [3]. The limitations of the available screening tests, combined with the often silent nature of NAFLD, may explain the substantial gap in the diagnosis of NAFLD.…”

Section: Introductionmentioning

confidence: 99%

A New, Non-Invasive Scale for Steatosis Developed Using Real-World Data From Russian Outpatients to Aid in the Diagnosis of Non-Alcoholic Fatty Liver Disease

Маев¹,

Самсонов²,

Лазебник³

et al. 2020

Adv Ther

View full text Add to dashboard Cite

Introduction: The current non-invasive tools for the diagnosis of non-alcoholic fatty liver disease (NAFLD) have methodological limitations. We aimed to develop a non-invasive scale to assist in the diagnosis of NAFLD. To achieve our aim, we conducted a secondary analysis of data from a large observational study conducted in Russia. Methods: This retrospective analysis assessed the frequency of NAFLD in the population of patients in the DIREG_L_06725 study, an epidemiological, observational, cross-sectional, multicenter study performed in 50,145 outpatients from 16 Russian cities. Among the cohort of patients diagnosed with NAFLD, we identified factors associated with the risk of NAFLD. To develop a non-invasive tool for diagnosing NAFLD, we also determined the frequency of steatohepatitis. Results: Our analysis included 48,297 patients; NAFLD was present in 20,281 patients (42.0%). The majority (64.1%) were women (80.3% postmenopause), and 87% had a body mass index (BMI) [ 27.0 kg/m 2. We developed a fully noninvasive scale (St-index) that showed a specificity of 91.4% for ruling in steatosis, and a sensitivity of 93.8% for ruling out steatosis. Multivariate regression analyses conducted in the subgroups of patients aged C 12 and \ 18 years and those with BMI \ 25.0 kg/m 2 produced area under the receiver operating characteristic (AUROC) curve values of 0.8243 and 0.7054, respectively. The factors most strongly associated with the development of NAFLD were age [ 35 years, presence of type 2 diabetes mellitus, and a waist circumference/ height ratio [ 0.54. Conclusion: Our non-invasive steatosis scale, St-index, can help physicians diagnose NAFLD in high-risk patients in the absence of ultrasound data.

show abstract

Imaging methods for screening of hepatic steatosis

Cited by 2 publications

References 77 publications

Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy

Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy

A New, Non-Invasive Scale for Steatosis Developed Using Real-World Data From Russian Outpatients to Aid in the Diagnosis of Non-Alcoholic Fatty Liver Disease

Contact Info

Product

Resources

About