Imaging modalities using magnetic nanoparticles – overview of the developments in recent years

Schwarz, Marc; Dörfler, Arnd; Engelhorn, Tobias; Struffert, Tobias; Tietze, Rainer; Janko, Christina; Tripal, Philipp; Cicha, Iwona; Dürr, Stephan; Lyer, Stefan

doi:10.1515/ntrev-2013-0010

Cited by 6 publications

(3 citation statements)

References 83 publications

(129 reference statements)

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“…Nanotechnology is of high potential for various medical applications, including cancer therapy [36], imaging [37], or tissue engineering [38]. However, just as any new technology, the use of nanoparticles in humans has concomitant risks.…”

Section: Discussionmentioning

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on Vocal Fold Fibroblasts: Cell Behavior and Cellular Iron Kinetics

et al. 2017

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PurposeThe voice is the most important instrument of communication. Tissue defects in the vocal fold (VF) area lead to serious reduction in quality of life, but thus far, no satisfactory VF implant exists. Therefore, we aim to establish a functional VF implant in a rabbit model by magnetic tissue engineering (MTE) using superparamagnetic iron oxide nanoparticles (SPION). Hence, iron quantification over time as well as cell behavior studies upon SPION treatment are of great importance.MethodsRabbit VF fibroblasts (VFF) were treated with different concentrations of SPIONs (20, 40, and 80 μg/cm2), and iron content was examined for up to 40 days using microwave plasma-atom emission spectroscopy. The effects of SPION treatment on VFF (adhesion, spreading, and migration), which are important for the formation of 3D structures, were tested.ResultsCellular SPION quantification revealed that there was no residual iron remaining in VFFs after 40 days. SPIONs had a dose-dependent effect on cell adhesion, with good tolerability observed up to 20 μg/cm2. Migration and spreading were not significantly influenced by SPION treatment up to 80 μg/cm2.Discussion and ConclusionTo develop 3D structures, cell behavior should not be affected by SPION uptake. After 40 days, cells were free of iron as a result of metabolism or rarefication during cell division. Cell functions including adhesion, spreading, and migration were proven to be intact in a dose-dependent manner after SPION treatment, suggesting a safe usage of MTE for voice rehabilitation. Our results thus constitute a solid basis for a successful transfer of this technique into 3D constructs, in order to provide an individual and personalized human VF implant in the future.

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Section: Discussionmentioning

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on Vocal Fold Fibroblasts: Cell Behavior and Cellular Iron Kinetics

et al. 2017

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“…[42][43][44][45] Interestingly, when combined with stimuli-responsive polymers, additional features-such as thermo-responsiveness, valuable for sensing and drug delivery applications for instance-are conferred to the hybrid systems. Thanks to their unique properties, stimuli-RMPHs are employed in a wide range of research domains, including energy storage, 33 solid-phase extraction, 7 catalysis, 39,46 sensing/imaging, 5,47 biomedical devices 48 and cancer theranostics. 12,40,49 The following sections of this review illustrate the various strategies reported in the literature to develop stimuli-RMPHs of different structures and shapes.…”

Section: Stimuli-responsive Magnetic Polymer Hybridsmentioning

confidence: 99%

Rational design of stimuli‐responsive magnetic polymer hybrid (nano)materials

Nguyen

Ménager

Rieger

et al. 2023

Polymer International

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Combining organic and inorganic materials is a fascinating strategy to produce hybrid materials that combine the advantages of both polymeric and inorganic materials. Among the various types of organic–inorganic hybrids, stimuli‐responsive magnetic polymer hybrids (RMPHs) are particularly promising materials for a wide variety of applications. While the magnetic properties are generally provided by the presence of magnetic nanoparticles, such as iron oxide nanoparticles, the polymeric compound brings the stimuli‐responsiveness, e.g. responsiveness to pH, temperature, redox reaction or irradiation. Furthermore, as the chemical structure and architecture of the polymeric materials are diverse and easily tunable, stimuli‐RMPHs have found applications in various domains, including catalysis, biotechnology, (bio)imaging and cancer therapy. Given the importance of the hybrids' shape and morphology for the targeted application, this review presents the possible synthetic strategies to rationally design stimuli‐RMPHs of various morphologies ranging from nanometric core–shell structures to nanogels, microgels and membranes. © 2023 The Authors. Polymer International published by John Wiley & Sons Ltd on behalf of Society of Industrial Chemistry.

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“…In vivo imaging technology may be used to dynamically observe the short-term distribution, homing and long-term survival of transplanted MSCs (4,5). The intravital tracer technique means that in stem cell transplantation, cells are able to be dynamically monitored in vivo to assess their migration and survival non-invasively (6–8). To obtain high definition images with high sensitivity, tracer technology for stem cells should be combined with multi-mode imaging (9).…”

Section: Introductionmentioning

confidence: 99%

In�vitro study on human umbilical cord mesenchymal stem cells transfected with lentivirus‑mediated hNIS‑EGFP dual reporter gene and co‑labeled with superparamagnetic iron oxide

et al. 2018

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The aim of the present study was to establish a stem cell line for multi-mode imaging (in vivo fluorescence imaging, magnetic resonance imaging and 99mTc single-photon emission computed tomography) and to study the biological activity, stemness, proliferative activity and differentiation ability of superparamagnetic iron oxide (SPIO), human sodium/iodide symporter (hNIS) and enhanced green fluorescent protein (EGFP) co-labeled human umbilical cord mesenchymal stem cells (hUCMSCs). The EGFP reporter gene was selected to indirectly reflect the expression of target gene hNIS, and hUCMSCs were re-transfected with the successfully constructed recombinant plasmid pCMV-NIS-EF1-GFP-PGK-puro. When a stem cell line stably expressing hNIS and EGFP was obtained, the cells were incubated with 30 µg/ml SPIO to obtain hNIS, EGFP and SPIO co-labeled stem cells. The protein expressions of hNIS and EGFP were identified using western blot analysis, and the protein function of hNIS was identified by 125I influx and 125I efflux experiments. hNIS-EGFP-hUCMSCs were labeled with SPIO under the mediation of poly-L-lysine, and SPIO, hNIS and EGFP co-labeled hUCMSCs were established successfully. Staining with Prussian blue confirmed that 98% of cells were successfully labeled with SPIO. Western blotting results demonstrated positive hNIS and EGFP protein expression levels, and 125I influx and 125I efflux experiments confirmed that the protein function of hUCMSCs after expressing hNIS was normal. The uptake of 125I was higher in cell lines hNIS-EGFP-hUCMSCs than in control hUCMSCs (fold change: 16.43±2.30 times; P<0.05). The stemness of hNIS-EGFP-hUCMSCs was found to be slightly decreased but not statistically significant; the overall characteristics of stem cells remained unchanged. The assessments of adipogenic and osteogenic differentiation suggest that hNIS-EGFP-hUCMSCs have no significantly different characteristics compared with primary hUCMSCs.

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Imaging modalities using magnetic nanoparticles – overview of the developments in recent years

Cited by 6 publications

References 83 publications

Impact of Superparamagnetic Iron Oxide Nanoparticles on Vocal Fold Fibroblasts: Cell Behavior and Cellular Iron Kinetics

Impact of Superparamagnetic Iron Oxide Nanoparticles on Vocal Fold Fibroblasts: Cell Behavior and Cellular Iron Kinetics

Rational design of stimuli‐responsive magnetic polymer hybrid (nano)materials

In�vitro study on human umbilical cord mesenchymal stem cells transfected with lentivirus‑mediated hNIS‑EGFP dual reporter gene and co‑labeled with superparamagnetic iron oxide

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