Imaging of angiogenesis using Cadence™ contrast pulse sequencing and targeted contrast agents

Stieger, Susanne M.; Dayton, Paul A.; Borden, Mark A.; Caskey, Charles F.; Griffey, Stephen M.; Wisner, Erik R.; Ferrara, Katherine W.

doi:10.1002/cmmi.224

Cited by 86 publications

(81 citation statements)

References 39 publications

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“…15,29 CPS method uses non-linear fundamental frequencies, which results in better spatial resolution and high sensitivity to contrast media, and provides improved tissue subtraction than previous ultrasound perfusion imaging techniques. 30,31 Transversal images of semimembranosus muscles of both limbs were captured after a single bolus (0.5 ml) of SonoVue contrast microbubbles (Bracco, Milan, Italy) with the following parameters at 8.0 MHz: power À16 dB, mechanical index 0.31, CPS gain À10 and depth 20 mm. Measurements were done once a week for the first month and thereafter twice a month, and the imaged area was the same in each time.…”

Section: Ultrasound Imagingmentioning

confidence: 99%

Long-term VEGF-A expression promotes aberrant angiogenesis and fibrosis in skeletal muscle

et al. 2011

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Section: Ultrasound Imagingmentioning

confidence: 99%

Long-term VEGF-A expression promotes aberrant angiogenesis and fibrosis in skeletal muscle

et al. 2011

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“…Then low power non-destructive pulses are applied to image the tMBs, followed by a high power disruptive pulse (flash) to eliminate the MBs within the imaging plane which is again followed by low power pulses to image the residual circulating MBs (Figure 2A). The intensity difference before and after the flash corresponds to the amount of bound tMBs and is a measure for the biomarker concentration [144][145][146][147], as shown in Figure 2A. In such methods the quantification of bound tMBs strongly depends on the injected dose, imaging system gain, and local perfusion [137].…”

Section: Contrast-specific Imaging Techniquesmentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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“…After intravenous injection, direct attachment of α V -targeted microbubbles to the vascular endothelium was evident and was significantly greater than adherence of nontargeted microbubbles. Two studies using a Matrigel plug model of angiogenesis demonstrated that the mAb strategy and the RGD peptide strategy yielded similar results, demonstrating similar efficacy of antibody-and peptide-based targeting strategies [12,16]. Next, in a clinically relevant rat model of chronic ischemia, angiogenesis was assessed by CEU molecular imaging after unilateral iliac artery ligation and after FGF-2 supplementation [17].…”

Section: Contrast-enhanced Ultrasound Molecular Imaging Of Angiogenesismentioning

confidence: 89%

Contrast Ultrasound and Targeted Microbubbles: Diagnostic and Therapeutic Applications for Angiogenesis

Smith

Fujii

Kuliszewski

et al. 2011

J. of Cardiovasc. Trans. Res.

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Angiogenesis represents the formation of new capillaries from existing vasculature, and as such plays a critical role in the response to ischemia in the setting of chronic coronary artery and peripheral vascular disease. Recent technological advances in non-invasive imaging modalities now allow the molecular imaging of angiogenesis. One such technique is contrast-enhanced ultrasound using microbubbles targeted against molecular markers of the angiogenic process. The ability to non-invasively image the angiogenic process would be useful in risk stratifying patients with arterial occlusive disease and would aid in the evaluation of new therapies to promote angiogenesis in ischemic cardiac and skeletal muscle. Furthermore, ultrasound technologies have also been developed that allow targeted angiogenic gene therapy using high-power ultrasound and DNA-bearing microbubbles. This review will focus specifically on recent advances in (1) contrast-enhanced ultrasound molecular imaging techniques for the evaluation of angiogenesis and (2) ultrasound-mediated gene delivery for therapeutic angiogenesis, techniques that have potential for translation to clinical practice.

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Imaging of angiogenesis using Cadence™ contrast pulse sequencing and targeted contrast agents

Cited by 86 publications

References 39 publications

Long-term VEGF-A expression promotes aberrant angiogenesis and fibrosis in skeletal muscle

Long-term VEGF-A expression promotes aberrant angiogenesis and fibrosis in skeletal muscle

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Contrast Ultrasound and Targeted Microbubbles: Diagnostic and Therapeutic Applications for Angiogenesis

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