Imaging of Ca^2+ intracellular dynamics with a third-harmonic generation microscope

Canioni, Lionel; Rivet, Sylvain; Sarger, L.; Barille, Régis; Vacher, Pierre; Voisin, Pierre

doi:10.1364/ol.26.000515

Cited by 46 publications

(31 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, inhomogeneities, in either refractive index or in χ (3) , within the focal volume can lead to a measurable third-harmonic signal. This is the contrast generating mechanism in THG microscopy, which has been demonstrated to be a useful imaging tool in biology and the material sciences [7][8][9][10][11][12][13][14][15][16]. Spherical aberration that results from focusing through a dielectric slab, can significantly distort the focal field of a high NA microscope objective [17].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation

Pillai¹,

Brakenhoff²,

Müller³

2006

Opt. Express

View full text Add to dashboard Cite

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using thirdharmonic generation. Pillai, R.S.; Brakenhoff, G.J.; Müller, M. Published in: Optics Express DOI:10.1364/OPEX.14.000260 Link to publication Citation for published version (APA):Pillai, R. S., Brakenhoff, G. J., & Müller, M. (2006). Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation. Optics Express, 14(1), 260-269. DOI: 10.1364/OPEX.14.000260 General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. uva.nl, sreedhar@science.uva.nl, muller@science.uva.nl Abstract: The third-order nonlinear susceptibility (χ (3) ) can be measured quantitatively using third-harmonic generation (THG) from two different interfaces. For the first time it is demonstrated both in experiments and theory that the magnitude of the THG signals from the two interfaces is not only determined by material properties (refractive index and χ (3) ), but also by optical aberrations. It is found that this method of χ (3) determination can be applied without additional correction factors only for focusing conditions with a numerical aperture (NA) ≤ 0.35. The implications for general application of THG in three-dimensional microscopy are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation

Pillai¹,

Brakenhoff²,

Müller³

2006

Opt. Express

View full text Add to dashboard Cite

show abstract

“…The combination of nonlinear optical spectroscopy with scanning microscopy generates innovative tools such as second-harmonic generation microscopy, 1-3 coherent antiStokes Raman scattering (CARS) microscopy, [4][5][6] and third-harmonic generation (THG) microscopy [7][8][9][10][11][12] for biology and materials science. THG microscopy was demonstrated by Barad et al in 1997.…”

Section: Introductionmentioning

confidence: 99%

“…7 This technique has been applied for imaging transparent objects, 7,8 laser-induced breakdown, 9 and biological samples. [10][11][12] The advantages of this technique are that it is capable of threedimensional sectioning and that there is no need to stain the sample.…”

Section: Introductionmentioning

confidence: 99%

Green's function formulation for third-harmonic generation microscopy

2002

View full text Add to dashboard Cite

We report a theoretical study of third-harmonic generation (THG) microscopy by use of a Green's function formulation. The third-harmonic signal under a tight-focusing condition is calculated for samples with various shapes and sizes. Our results show that THG signals can be efficiently generated at a sizable interface perpendicular or parallel to the optical axis or from a small object with a size comparable to the width of the axial excitation intensity profile. The signal-generation mechanism of THG microscopy is explained by a modified phase-matching condition, ͉k 3 Ϫ 3(k 1 ϩ ⌬k g )͉l Ӷ , where ⌬k g is the wave vector mismatch induced by the Gouy phase shift of the focused excitation field. The relation of the THG power and radiation pattern to the orientation of an interface is investigated. A comparison between signal generation in THG microscopy and that in coherent anti-Stokes Raman scattering microscopy is given.

show abstract

“…The relative ease of generating laser pulses with durations of 100 femtoseconds (fs) to a few picoseconds (ps) made it possible to explore nonlinear optical effects as means of contrast generation in label-free microscopy. The most straightforward of these approaches rely on the detection of the second-harmonic (SHG) [6][7][8] or the third-harmonic generation (THG) of the excitation beam. [9][10][11] Whereas SHG is surface-specific and requires a local break of the inversion symmetry, THG is sensitive to interfaces in either third-order nonlinear susceptibility and/or refractive index.…”

Section: Introductionmentioning

confidence: 99%

Coherent anti‐Stokes Raman Scattering Microscopy

2007

View full text Add to dashboard Cite

Coherent anti‐Stokes Raman scattering (CARS) microscopy is presented as a new nonlinear optical technique. The combination of vibrational spectroscopy and microscopy allows highly sensitive investigations of unlabelled samples. CARS is an ideal tool for studying a broad variety of samples. The main drawback of the technique is its non‐zero‐background nature, which implies that the signal has to be detected against a nonresonant background. The need to solve this problem is reflected in the rapid technological developments that have been observed during the last decade. Recent results show that CARS microscopy has the potential to become an important complementary technique that can be used with other well‐established microscopic methods. Although it has some limitations, it offers unique access to many problems that cannot be tackled with conventional techniques. For this reason, it can be expected that the impressive growth of the field will continue.

show abstract

Imaging of Ca^2+ intracellular dynamics with a third-harmonic generation microscope

Cited by 46 publications

References 11 publications

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation

Green's function formulation for third-harmonic generation microscopy

Coherent anti‐Stokes Raman Scattering Microscopy

Contact Info

Product

Resources

About